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Plant Cell Culture Initiation: Practical Tips

Molecular Biotechnology, 2000
The use of cultured plant cells in either organized or unorganized form has increased vey considerably in the last 10-15 yr. Many new technologies have been developed and applications in both fundamental and applied research have led to the development of some powerful tools for improving our knowledge of botanical systems and for gaining external ...
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Protein kinase in cultured plant cells

Biochimica et Biophysica Acta (BBA) - Enzymology, 1975
A protein kinase (EC 2.7.1.37) which phosphorylates histones was purified partially from the soluble fractions of cultured plant cells. The optimum pH was 7.5 to 9.0. The activity wasnot stimulated by exogeneous cyclic AMP. It was thermolabile and completely dependent on the presence of Mg2+ or Mn2+ for activity.
N, Nakaya   +3 more
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Verbascoside production by plant cell cultures

Plant Cell Reports, 1991
Verbascoside was found to be produced in all calli derived from eleven species that contained the compound in their leaves. Cell suspension cultures were also established in three species, i.e., Leucosceptrum japonicum f. barbinerve, Syringa josikaea, and Sy. vulgaris, all of which were found to produce verbascoside at more than 1 g/l.
N, Inagaki   +3 more
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Tocopherol production in plant cell cultures

Molecular Nutrition & Food Research, 2010
AbstractTocopherols, collectively known as vitamin E, are lipophilic antioxidants, essential dietary components for mammals and exclusively synthesized by photosynthetic organisms. Of the four forms (α, β, γ and δ), α‐tocopherol is the major vitamin E form present in green plant tissues, and has the highest vitamin E activity.
Caretto S, Nisi R, Paradiso A, De Gara L
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Plant cell culture technologies

Botanical Journal of Scotland, 1991
Summary The commercial exploitation of plant cell, tissue and organ cultures is now a reality and the new technologies are already in place and developing rapidly. Their emergence has provided new perspectives and sharpened the focus of the ways in which plant cell and tissue culture can aid man.
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Dual Fungal and Plant Cell Culture

2003
Plant tissue cultures are now well-recognized as valuable experimental systems for use in the study of host-pathogen interactions. These techniques have obvious major advantages for the examination of obligately biotrophic fungi and also those with a necrotrophic life style, and it is in these areas that much research effort has been concentrated (1 ...
A, Donovan, S, Isaac, H A, Collin
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Large-Scale Culture of Plant Cells

2003
The large-scale or mass cultivation of plant cells is the growth of plant cell suspensions at volumes above those normally produced in shake flasks, that is, above IL. Attempts to grow plant cells in fermenters or bioreactors started in the early 1960s with converted carboys.
A H, Scragg, M W, Fowler
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Culture establishment, plant cell

2010
Alternatively to whole plants, plant cell cultures are used to produce bioactive substances for food industry, cosmetics and pharmacy. This mainly concerns secondary metabolites and recombinant proteins (so-called plant made proteins, PMPs). Among the employed culture types (which comprise suspension cultures, root cultures and shoot cultures), plant ...
Eibl, Regine   +4 more
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Integrated Bioprocessing for Plant Cell Cultures

2001
Plant cell suspension culture has become the focus of much attention as a tool for the production of secondary metabolites including paclitaxel, a well-known anticancer agent. Recently, it has also been regarded as one of the host systems for the production of recombinant proteins. In order to produce phytochemicals using plant cell cultures, efficient
J W, Choi, G H, Cho, S Y, Byun, D I, Kim
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Plant Cell Culture Protocols

2012
Part I. Introduction An Introduction to Plant Cell Culture: Back to the Future Victor M. Loyola-Vargas and Felipe Vazquez-Flota History of Plant Tissue Culture Trevor A. Thorpe Part II. Cell Culture and Plant Regeneration: The Fundamentals Pathogen and Biological Contamination Management: The Road Ahead Alan C. Cassells and Barbara M.
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