Results 151 to 160 of about 465,401 (218)

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Microbial Volatile Organic Compounds (mVOCs) from rhizobacterium RL-WG62 mediate salt tolerance in Brassica napus by reprogramming transcriptome. [PDF]

BMC Plant Biol
Wang Y, Tan L, Chen J, Wang H, Hu H, Zhang C, Ren L.   +6 more
europepmc   +1 more source

Combined 5‐aminolevulinic acid and ferric ammonium citrate treatment promotes hair follicle growth by activating dermal papilla cells

FEBS Open Bio, EarlyView.
5‐Aminolevulinic acid combined with ferric ammonium citrate (5‐ALA/FAC) stimulates dermal papilla cell activity and promotes hair follicle growth. The treatment enhances ERK and AKT signaling, increases hair‐inductive gene expression, and restores dermal papilla function suppressed by dihydrotestosterone and oxidative stress, resulting in enhanced hair
Han‐Wook Ryu, Eok‐Soo Oh, Sewoon Kim
wiley   +1 more source

Effect of medium composition on in vitro ovary culture of cucumber. [PDF]

Sci Rep
Nyirahabimana F, Erol MH, Kaçar YA, Solmaz İ.   +3 more
europepmc   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Genomic Insights and Antifungal Efficacy of <i>Xenorhabdus budapestensis</i> XH-4 in Combating Soybean Root Rot. [PDF]

J Fungi (Basel)
Qu Y, Li K, Wang Z, Dong H, Hategekimana A, Wang X, Yin J.   +6 more
europepmc   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Indole-3-Acetic Acid Biosynthesis by Endophytic Fungi and Its Association with Plant-Fungus Interactions. [PDF]

J Fungi (Basel)
Turbat A, Vigneshwari A, Huynh T, Oyunbat N, Tiwari A, Ramteke PW, Varga M, Papp T, Vágvölgyi C, Szekeres A.   +9 more
europepmc   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

A Two-Phase System for the In Vitro Culture of <i>Agave guiengola</i> Gentry. [PDF]

Plants (Basel)
Maldonado-Zavala I, Castañeda-Nava JJ, Delgado-Aceves L, Rodríguez-Domínguez JM, Rodríguez-Garay B, Gutiérrez-Mora A.   +5 more
europepmc   +1 more source