Yeast Gcn2 retains activity following humanization of its auto‐phosphorylation region
Using Saccharomyces cerevisiae as a model to study Gcn2 activation and regulation is limited by the lack of antibodies detecting phosphorylated Gcn2. To overcome this, we engineered Gcn2‐HsC, a yeast Gcn2 variant recognizable by commercial anti‐human phospho‐GCN2 antibodies.
Reuben A. Anderson +2 more
wiley +1 more source
Metabolic Profiling Using Gas Chromatography-Mass Spectrometry and Optimization of Light-Emitting Diodes for Callus Induction in Patchouli "Tapak Tuan" (Pogostemon cablin Benth.). [PDF]
Harnelly E +4 more
europepmc +1 more source
Effect of terahertz irradiation on DNA damage repair in living cells
We investigated the effect of terahertz (THz) wave irradiation on DNA double‐strand break (DSB) repair in living cells. We found that THz irradiation enhanced DSB repair at specific frequencies, whereas heat treatment inhibited it, indicating that this effect is nonthermal and frequency‐specific.
Yuya Ueno +3 more
wiley +1 more source
<sup>1</sup>H-NMR-Based Metabolomic Study of Tomato Cultivars, Heinz and Roma, Grown on Selected Growth Medium and Deep-Water Hydroponic Culture System. [PDF]
Nsele SN +3 more
europepmc +1 more source
Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana +5 more
wiley +1 more source
Culture-based inventory and functional guild analysis of root-invading fungi associated with grassland plants across multiple regions in China. [PDF]
Wang TT +5 more
europepmc +1 more source
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas +6 more
wiley +1 more source
Organ-on-a-Chip and Microfluidic Plant Cell Culture Systems: The Next Frontier for Controlled Secondary Metabolite Production and Real-Time Metabolomic Monitoring. [PDF]
Dadhich A, Sharma V, Sivanesan I.
europepmc +1 more source
Malformin A1–mediated cytotoxicity in ovarian cancer cells occurs through pyroptosis and autophagy
This study investigated the effects of the natural compound Malformin A1 (MA1) on the cytoskeleton that regulates cell proliferation and migration. Disruption of the cytoskeleton can impair these processes and promote cancer cell death. MA1 disrupted cytoskeletal organization, induced DNA damage, inflammation, activated autophagy, and pyroptosis ...
Nada Abdullah Hassan +11 more
wiley +1 more source
<i>In vitro</i> micrografting success and histological union characterization of <i>Argania spinosa</i> (L.) skeels scion lines grafted onto rootstock G54. [PDF]
Fathellah H +7 more
europepmc +1 more source

