Influence of the In Vitro Culture System on the Preferential Accumulation of Bioactive Compounds in <i>Stevia rebaudiana</i> Shoots. [PDF]
Martínez-Oró D +2 more
europepmc +1 more source
In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Hairy Roots Cultures from the Slow-Growing and Protected <i>Panax ginseng</i>, <i>Leontopodium alpinum</i> and <i>Gynostemma pentaphyllum</i> as an Alternative Source of Antimicrobials. [PDF]
Braissant O, Flury V, Jobin M, Hu S.
europepmc +1 more source
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Mitigation of serotonin-caused suppression of osteoblast function by extracts of the psychoactive indigenous plant Sceletium tortuosum. [PDF]
Schröder HC +10 more
europepmc +1 more source
The brown marmorated stink bug, Halyomorpha halys, mainly secretes 4‐oxo‐(E)‐2‐hexenal, (E)‐2‐decenal, dodecane and tridecane, a mixture representative of that released by many heteropterans. We demonstrated that H. halys can synthesise these secretory components de novo using dietary glucose.
Haruna Fujimori, Koji Noge
wiley +1 more source
Phosphorus and Potassium Mobilization from Rock-Derived Mineral Sources Using Bacteria and Microalgae: A Review. [PDF]
Simon V +6 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Establishment of Tissue Culture and Genetic Transformation Systems Using Mature Embryos of Bread Wheat. [PDF]
He Y +9 more
europepmc +1 more source
Tailoring LED spectra and cultivation systems enhances lettuce productivity and nutritional quality in vertical indoor farming. [PDF]
Aldiyab A +6 more
europepmc +1 more source

