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Plant Protein Extraction

2007
A method is presented for the extraction of total protein from Arabidopsis thaliana tissue. The protocol was designed for the solubilization of a range of proteins and their efficient and quantitative recovery. It is especially compatible with the small quantities of available tissue often associated with this species and was originally intended for ...
Helen E, Conlon, Michael G, Salter
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Extraction of Plant RNA

2007
This protocol details an RNA preparation for medium-scale, high-purity RNA production from higher plants. It uses hot acid phenol with standard sodium acetate ethanol precipitation and is suitable for producing RNA for both Northern blotting and enzyme-based downstream applications such as RT-PCR and microarray studies.
Michael G, Salter, Helen E, Conlon
openaire   +4 more sources

Extractive Plant Cell Culture

Current Opinion in Biotechnology, 1992
In recent years suspension cultures of plant cells have been used to produce many biochemicals, including perfumes, dyes, medicinals, and opiates. This technology is progressing rapidly because of its advantages in terms of simplicity and potential economics compared to extraction from natural plants.
Ho Nam Chang, Sang Jun Sim
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Tumour Production in Plants by Plant Extracts

Nature, 1959
THE importance of purified forms of alkaloids in inducing plant tumours is well known. Very little work has however been done on the use of plant extracts, containing alkaloids, in tumour production. Takenaka1 has summarized the meagre data available and noted the effects of two plant extracts on living plant tissue.
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Protoheme extraction from plant tissue

Analytical Biochemistry, 1978
A method for reproducibly estimating the protoheme content of plant tissues has been developed. The tissue sample is homogenized in 80% acetone to remove pigments and lipids; protoheme is then extracted from the tissue residue with 2% HCl in acetone and quantitatively transferred into diethyl ether.
L C, Stillman, M L, Gassman
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Metabolomic fingerprinting of plant extracts

Journal of Mass Spectrometry, 2006
AbstractThe standardization and quality control of plant extracts is an important topic, in particular, when such extracts are used for medicinal purposes. Consequently, the development of fast and effective analytical methods for metabolomic fingerprinting of plant extracts is of high interest.
MATTOLI L   +8 more
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Membrane Extracts from Plant Tissues

2020
The comparison of isolated plant cell membranous enclosures can be hampered if their extraction method differs, e.g., in regard to the utilized buffers, the tissue, or the developmental stage of the plant. Thus, for comparable results, different cellular compartments should be isolated synchronously in one procedure.
Uwe, Bodensohn   +3 more
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Plant extracts in BPH.

Minerva urologica e nefrologica = The Italian journal of urology and nephrology, 1993
In Italy plant extracts represent 8.6% of all pharmacological prescriptions for Benign Prostatic Hyperplasia (data from 1991). This review evaluates all the suggested mechanisms of action for plant extracts. Recently we demonstrated an antiestrogenic effect of Serenoa Repens in BPH patients.
DI SILVERIO, Franco   +7 more
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Plant Tissue Extraction for Metabolomics

2013
Plants are not only important producers of foods and energy storages (e.g., sugars, carbohydrates, proteins, and fats) in the form of grains, fruits, and vegetables, they also provide many valuable products to human existence including wood, fibers, oils, resins, pigments, antioxidants, and sources of medicine.
Ute, Roessner, Daniel Anthony, Dias
openaire   +2 more sources

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