CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Multiclass semantic segmentation for prime disease detection with severity level identification in Citrus plant leaves. [PDF]
Dinesh P, Lakshmanan R.
europepmc +1 more source
Green synthesis of multi-functional carbon dots from medicinal plant leaves for antimicrobial, antioxidant, and bioimaging applications. [PDF]
Gedda G +5 more
europepmc +1 more source
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
Thin, Flexible Humidity Sensors with Self-Doped Organic Conducting Polymers Waterproofed by Cross-Linked Polyvinyl alcohol for Monitoring Transpiration on Plant Leaves. [PDF]
Okonogi A +7 more
europepmc +1 more source
Bibliography: leaves 113-134.A brief review of the literature on the effects of nitrate and ammonium nitrogen sources on plant growth, and the assimilation of those nitrogen sources, has been presented.
Murphy, Andrew Telfer
core
Nuclear pore links Fob1‐dependent rDNA damage relocation to lifespan control
Damaged rDNA accumulates at a specific perinuclear interface that couples nucleolar escape with nuclear envelope association. Nuclear pores at this site help inhibit Fob1‐induced rDNA instability. This spatial organization of damage handling supports a functional link between nuclear architecture, rDNA stability, and replicative lifespan in yeast.
Yamato Okada +5 more
wiley +1 more source
Effects of Pregelatinization on the Physicochemical Properties of Corn Grits and the Quality of Cooked Waxy Corn Wrapped in Plant Leaves. [PDF]
Wang Y +7 more
europepmc +1 more source
Monitoring of the copper persistence on plant leaves using pulsed thermography. [PDF]
Rippa M +5 more
europepmc +1 more source
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source

