Results 101 to 110 of about 507 (180)

Bioimage analysis in deep visual proteomics: Advancing transparency, reproducibility, and FAIR principles

Journal of Microscopy, EarlyView.
Abstract Deep Visual Proteomics (DVP) combines high‐resolution microscopy, computational instance segmentation, laser capture microdissection (LMD), and ultrasensitive mass spectrometry to enable spatially resolved molecular analysis of defined cellular populations.
Devon Siemes, Angelo Novak, Stephanie Thiebes, Lars Widera, Stephanie Tautges, Hannah Voss, Lars Borgards, Dominik Habermann, Jianxu Chen, Olga Shevchuk, Daniel R. Engel   +10 more
wiley   +1 more source

Knocking Out Two Polyphenol Oxidase Genes Significantly Improves Recombinant Protein Purification in Nicotiana benthamiana

Plant Biotechnology Journal, EarlyView.
ABSTRACT Efficient purification remains a key technical challenge affecting the recovery efficiency of recombinant proteins in plant‐based systems. Complex metabolites, particularly polyphenols, often cause recombinant protein aggregation during purification.
Hai‐Ping Diao, Hui‐Xin Meng, Xue‐Jiao Xu, Zeng‐Lin Zhang, Jian‐Feng Zhang, Yong‐Feng Guo, Inhwan Hwang, Shi‐Jian Song   +7 more
wiley   +1 more source

Heterologous production of oligopeptides with antihypertensive effects in Lactuca sativa and Medicago truncatula [PDF]

, 2018
“In the current scenario of a worldwide problematic situation related to cardiovascular diseases associated with hypertension, the necessity for alternative therapies has become a great challenge.
Oliveira, Filipe Silva Nunes de
core  

Rituximab Molecular Pharming: Designing a vector that yields high monoclonal antibody Rituximab levels through plant transformation [PDF]

Plant transformation for protein expression is a rapidly expanding method in biomedical sciences, providing a means of replicating therapeutic proteins that hold limits in their reproducibility.
McCready Martin, Zoe
core   +2 more sources

Multi‐Omics Analyses Reveal the Red and Far‐Red Light Combination Enhancing Heterologous Protein and Metabolite Production in Nicotiana benthamiana

Plant, Cell &Environment, EarlyView.
ABSTRACT Transient expression of exogenous protein in Nicotiana benthamiana leaves via agroinfiltration offers a rapid and efficient platform for functional gene discovery and heterologous production of valuable eukaryotic proteins and metabolites. Though light quality is an important factor for plant photomorphogenesis, its impact on the efficiency of
Yating Zhang, Fengjiao Wang, Ran Du, Tao Li, Shaoqun Zhou, Jianbin Yan, Wei Li   +6 more
wiley   +1 more source

Interaction Among Microbiota, Chemical Composition, and Quality Variations in Coffea arabica Across Altitudinal Gradients During Wet Processing

Food Science &Nutrition, Volume 14, Issue 8, August 2026.
Fermentation significantly altered the dominant bacterial genera from Sphingomonas and Pleomorphomonas (before fermentation) to Weissella and Lactobacillus (after fermentation), while Cladosporium and Fusarium remained the dominant fungal genera. Notably, non‐volatile compounds in de‐pulping coffee beans and volatile compounds of roasted coffee beans ...
Xiaojing Shen, Yamei Wu, Qi Wang, Wenhua Chen, Bintao Peng, Surui Lu, Ying Yang, Kunyi Liu, Jilai Zhang   +8 more
wiley   +1 more source

Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco [PDF]

, 2013
Includes abstract.Includes bibliographical references.Plants are emerging as cost friendly alternative production systems for a variety of pharmaceuticals. Numerous therapeutic proteins have been produced in plant systems (Giddings et al., 2000; Ma et al.
Stark, Hester Catharina (Therese)
core  

Effects of Bifidobacterium animalis Subsp. Lactis CP‐9 on Gut Microbiota and Immune Functions in Healthy Infants

Microbial Biotechnology, Volume 19, Issue 8, August 2026.
This study evaluated the 3‐month safety and tolerability of the probiotic CP‐9 in healthy infants aged 6 to 36 months. The research confirmed that, under the conditions of this study, CP‐9 can safely enrich specific beneficial bacterium in the gut without any obvious short‐term safety signal. ABSTRACT Infancy represents a critical period for growth and
Liujing Zhang, Xueke Song, Keming Zhang, Xiaoshuang Xu, Xinmei Guo, Wei Li, Xiaoling Zeng, Yingguang Xie, Liwei Nie, Li Zhao, Xuelu Dai, Jing Sun, Jiai Yan, Yiran Liu, Feng Zhang, Zhiping Sun   +15 more
wiley   +1 more source

Inflammatory Cytokines Impair Glucagon Expression and Secretion in Pancreatic α‐Cells

Diabetes, Obesity and Metabolism, Volume 28, Issue 8, Page 7149-7160, August 2026.
ABSTRACT Aims Insulin resistance and obesity‐associated inflammation are key drivers in the pathogenesis of Type 2 diabetes mellitus (T2DM). Whilst inflammatory cytokines are well known to impair β‐cell function, their impact on pancreatic α‐cells and glucagon (GCG) regulation remains poorly understood. In this study, we investigated the effects of the
Caroline Bickelmann, Cedric Wilden, Leticia Prates‐Roma, Matthias W. Laschke, Emmanuel Ampofo, Selina Wrublewsky   +5 more
wiley   +1 more source

Dietary N‐Acetylserotonin offers protection against Shewanella putrefaciens infection in Chinese mitten crab Eriocheir sinensis

Journal of the World Aquaculture Society, Volume 57, Issue 4, August 2026.
Abstract Shewanella putrefaciens is an important bacterial pathogen that poses a serious threat to Chinese mitten crab (Eriocheir sinensis) aquaculture. However, the protective effects of N‐Acetylserotonin (NAS) against S. putrefaciens infection in E. sinensis remain unknown.
Xiuxian Zhang, Yiyao Liu, Xurui Zheng, Meiling Liu, Li He, Sijia Chen, Haipeng Cao, Chunlei Gai, Weidong Ye   +8 more
wiley   +1 more source