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Plant Regeneration: Morphogenesis
1994The ability to regenerate whole plants from selected or genetically altered cells or tissues is the key to the broad biotechnological potential of plant cell cultures. This is not a fundamentally novel process, it has long been used commercially to propagate and produce certain cultures, e.g.
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Plant regeneration from callus cultures of Lithospermum erythrorhizon
Plant Cell Reports, 1997We previously studied the production of shikonin derivatives by cell lines ofLithospermum erythrorhizon. As a result, we have obtained a cell line LE 87, which exhibited high cell growth and high shikonin production. In the present study, the effects of auxins (2,4-D, IAA, picloram, and NAA) and cytokinins (BAP and kinetin) on organogenesis and somatic
Hee-Ju, Yu +5 more
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Plant regeneration from mesophyll protoplasts ofDianthus superbus
Plant Cell Reports, 1996Leaf mesophyll protoplasts ofDianthus superbus were cultured at a density of 5 × 10(4) protoplasts/ml and divided at about 18% plating efficiency in MS liquid medium supplemented with 0.5 mg/L BAP, 2.0 mg/L NAA and 9% mannitol after 2 weeks. Protocolonies formed after 3 to 4 weeks of culture in the dark at 27°C.
J C, Kim, E A, Lee
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Transformation and Regeneration of Dicotyledonous Plants
2003The formation of crown galls on many dicotyledonous plants, as a result of Agrobacterium tumefaciens infection, has been well documented (1). The transfer of a small T-DNA region of the Ti plasmid to the nuclear genome of the plant host (2) and expression of the T-DNA growth hormone genes are responsible for the unorganized habit characteristic of ...
K M, Gartland, N W, Scott
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Cell wall remodeling during plant regeneration
Journal of Integrative Plant BiologyABSTRACTPlant regeneration is the process during which differentiated tissues or cells can reverse or alter their developmental trajectory to repair damaged tissues or form new organs. In the plant regeneration process, the cell wall not only functions as a foundational barrier and scaffold supporting plant cells but also influences cell fates and ...
Guifang Zhang +6 more
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Plant regeneration from somatic embryos of Taxus brevifolia
Plant Cell Reports, 1996Taxusbrevifolia is the source of paclitaxel (Taxol®), an anticancer drug. A method for regeneration ofTaxus brevifolia from immature zygotic embryos via somatic embryogenesis is described. Embryogenic callus tissues were obtained by culturing immature zygotic embryos on Lloyd and McCown medium (MCM) supplemented with 160 μM 2,4-dichlorophenoxyacetic ...
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Plant regeneration: REF1 calls the fouls
Current BiologyRegenerative organisms such as plants must have specific signals that respond to damage and instruct remnant tissue to undergo repair. A recent paper identifies a long-sought candidate for the signal that links injury to regenerative programs.
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