Results 81 to 90 of about 28,405 (219)

Reconstructing enzyme evolution by protein engineering

FEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler, Markus R. Busch, Reinhard Sterner   +2 more
wiley   +1 more source

Decoding the dynamic extracellular matrix in cancer—3D models and bioscaffolds rewire the rules of tumor progression

FEBS Letters, EarlyView.
Cancer progression is regulated by the dynamic matrix code of the tumor microenvironment, which influences cellular behavior and disease development. Importantly, matrix remodeling in three‐dimensional cancer models more accurately reflects in vivo conditions compared to conventional two‐dimensional systems.
Sylvia Mangani, Konstantinos Spanopoulos, Zoi Piperigkou, Nikos K. Karamanos   +3 more
wiley   +1 more source

Toxicity evaluation of long-term crumb rubber leachate in ryegrass roots: Morphology, stress physiology and cell structure

Ecological Indicators
While significant attention has focused on the recycling of waste rubber, the toxicological risks associated with waste rubber have not been adequately addressed.
Li’an Duo, Mengjia Ding, Yuan Li, Qi Wang, Shulan Zhao   +4 more
doaj   +1 more source

Plant resistance to plant diseases [PDF]

, 2000
Plants are continuously exposed to the attack of several thousand of different strains of fungi, bacteria, viruses and other microorganisms. A plant can be attacked by thousands, hundreds and in the case of leaf spot in woody plants, even by hundreds of thousands of a single pathogen individuals.
Jakovljević, Dragana, Babović, Milorad, Delibašić, Goran   +2 more
openaire  

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

PsAF5 functions as an essential adapter for PsPHB2-mediated mitophagy under ROS stress in Phytophthora sojae

Nature Communications
Host-derived reactive oxygen species (ROS) are an important defense means to protect against pathogens. Although mitochondria are the main intracellular targets of ROS, how pathogens regulate mitochondrial physiology in response to oxidative stress ...
Wenhao Li, Hongwei Zhu, Jinzhu Chen, Binglu Ru, Qin Peng, Jianqiang Miao, Xili Liu   +6 more
doaj   +1 more source

Conserved binding mode but diverse interfaces of MreC‐PBP2 interactions

FEBS Letters, EarlyView.
The crystal structure of abMreC reveals a conserved two β‐barrel architecture and provides structural insights into its role within the bacterial elongasome. The abMreC–abPBP2 complex model identifies the molecular basis of MreC‐mediated PBP2 recognition, contributing to the regulation of peptidoglycan synthesis.
Hyunseok Jang, Hyo Been Jin, Chang Min Kim, So Yeon Lee, Hyun Ho Park   +4 more
wiley   +1 more source

Mechanism of tea infusion as a novel control agent against Fusarium graminearum

Phytopathology Research
Fusarium head blight (FHB), caused by Fusarium graminearum, severely reduces wheat yield and grain quality due to deoxynivalenol (DON) contamination. To develop sustainable alternatives to synthetic fungicides, we evaluated the antifungal activity of tea
Ying Li, Xintong Wang, Hongfei Li, Ziyi Cui, Jiahui Kan, Ming Qin, Qiang Li, Baotong Wang   +7 more
doaj   +1 more source

Microbiome‐blood–brain barrier interactions in aging — mechanisms and therapeutic potential

FEBS Letters, EarlyView.
Aging reshapes the gut microbiome (↓SCFA‐producing commensals; ↑pro‐inflammatory outputs), shifting circulating metabolites (↓SCFAs; ↑LPS, ↑TMAO, ↑PAA) that act at the BBB to increase nonspecific transcytosis, alter transport, and promote astrocyte reactivity, heightening brain vulnerability.
Daniel Cuervo‐Zanatta, Hari Baskar Balasubramanian, Amir Pasokh, Marietta Zille   +3 more
wiley   +1 more source

Virulence and genetic characterization of Puccinia striiformis f.sp. tritici populations on different wheat cultivars

Phytopathology Research
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a major global threat to wheat production, severely affecting yield stability.
Jierong Wang, Yongjin Jin, Li Geng, Hongyan Wang, Jing Zhao, Lili Huang, Zhensheng Kang, Gangming Zhan   +7 more
doaj   +1 more source