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Data Science and Plant Metabolomics
The study of plant metabolism is one of the most complex tasks, mainly due to the huge amount and structural diversity of metabolites, as well as the fact that they react to changes in the environment and ultimately influence each other. Metabolic profiling is most often carried out using tools that include mass spectrometry (MS), which is one of the ...
Anna Kisiel +3 more
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Protocol for diagnosing Erwinia amylovora infection using a fluorescent probe
Summary: Current fire blight diagnosis techniques are DNA based and require specialized equipment and expertise, or they are less sensitive. Here, we present a protocol for diagnosing fire blight using the fluorescent probe, B-1.
Ji Hye Jin, Dokyoung Kim
doaj +1 more source
The botanical content in the South African curriculum: A barren desert or a thriving forest?
Botanists who are interested in education have often expressed their dismay at how plant sciences are neglected in Biology curricula, despite the important roles that plants play.
Amelia L. Abrie
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GhPIPLC2D promotes cotton fiber elongation by enhancing ethylene biosynthesis
Summary: Inositol-1,4,5-trisphosphate (IP3) is an important second messenger and one of the products of phosphoinositide-specific phospholipase C (PIPLC)-mediated phosphatidylinositol (4,5) bisphosphate (PIP2) hydrolysis.
Liping Zhu +5 more
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INTRODUCTION The practice of publishing supplementary materials with journal articles is becoming increasingly prevalent across the sciences. We sought to understand better the content of these materials by investigating the differences between the ...
Edward Flathers +2 more
doaj +2 more sources
Summary: Calonectria hemileiae, a fungus associated with pustules of the coffee leaf rust (CLR, Hemileia vastatrix) in Brazil, was tested in vitro and in planta to assess its biocontrol potential.
Sara Salcedo-Sarmiento +8 more
doaj +1 more source
Protocol to fluorescently stain vacuoles in Arabidopsis root cells
Summary: Plant vacuoles are essential organelles that respond to developmental and environmental signals. Here, we present a protocol for staining the vacuole lumen in Arabidopsis root cells, enabling precise visualization of vacuolar dynamics.
Wylie Kristopher Tiernan +3 more
doaj +1 more source
Summary: Here, we present a protocol for generating and selecting stable transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation of cotyledon and hypocotyl explants.
Fanourios Mountourakis +2 more
doaj +1 more source
Summary: Plant virus vectors have emerged as promising tools for CRISPR-Cas reagent delivery. Here, we present a protocol for DNA-free plant genome editing using an engineered RNA virus vector for the transient delivery of CRISPR-Cas components.
Huanhuan Lou +8 more
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ABSTRACT Background Children with sickle cell disease (SCD) face multiple acute and chronic medical complications that may impact their quality of life as reported by patients themselves. Health‐related social needs (HRSNs), such as food and housing insecurity, are common in people with SCD, but the association between HRSNs and patient‐reported ...
Sarah J. Marks +5 more
wiley +1 more source

