Results 31 to 40 of about 14,169,433 (166)

The Journal Article as a Means to Share Data: a Content Analysis of Supplementary Materials from Two Disciplines

Journal of Librarianship and Scholarly Communication, 2016
INTRODUCTION The practice of publishing supplementary materials with journal articles is becoming increasingly prevalent across the sciences. We sought to understand better the content of these materials by investigating the differences between the ...
Edward Flathers, Jeremy Kenyon, Nancy Sprague   +2 more
doaj   +2 more sources

Elucidating the interactions between the rust Hemileia vastatrix and a Calonectria mycoparasite and the coffee plant

iScience, 2021
Summary: Calonectria hemileiae, a fungus associated with pustules of the coffee leaf rust (CLR, Hemileia vastatrix) in Brazil, was tested in vitro and in planta to assess its biocontrol potential.
Sara Salcedo-Sarmiento, Carlos E. Aucique-Pérez, Patrícia R. Silveira, Adans A. Colmán, André L. Silva, Paloma S. Corrêa Mansur, Fabrício Á. Rodrigues, Harry C. Evans, Robert W. Barreto   +8 more
doaj   +1 more source

Protocol to fluorescently stain vacuoles in Arabidopsis root cells

STAR Protocols
Summary: Plant vacuoles are essential organelles that respond to developmental and environmental signals. Here, we present a protocol for staining the vacuole lumen in Arabidopsis root cells, enabling precise visualization of vacuolar dynamics.
Wylie Kristopher Tiernan, Graciela Veronica Castro, Kurtis Shipman, Cecilia Rodriguez-Furlan   +3 more
doaj   +1 more source

Protocol for generating and selecting transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation

STAR Protocols
Summary: Here, we present a protocol for generating and selecting stable transgenic tomato lines through Agrobacterium tumefaciens-mediated transformation of cotyledon and hypocotyl explants.
Fanourios Mountourakis, Sotirios Fragkostefanakis, Panagiotis Nikolaou Moschou   +2 more
doaj   +1 more source

Protocol for transformation-free genome editing in plants using RNA virus vectors for CRISPR-Cas delivery

STAR Protocols
Summary: Plant virus vectors have emerged as promising tools for CRISPR-Cas reagent delivery. Here, we present a protocol for DNA-free plant genome editing using an engineered RNA virus vector for the transient delivery of CRISPR-Cas components.
Huanhuan Lou, Haiying Xiang, Wanli Zeng, Jiarui Jiang, Jianduo Zhang, Li Xu, Chenglu Zhao, Qian Gao, Zhenghe Li   +8 more
doaj   +1 more source

Protocol to monitor activation of a two-component system in Dickeya dadantii during chicory leaf infection using Phos-tag gel

STAR Protocols
Summary: Quantifying the phosphorylation levels of proteins involved in bacterial signaling pathways is essential for understanding their role in stress adaptation and infection processes.
Edwige Madec, Jean-Marie Lacroix, Sébastien Bontemps-Gallo   +2 more
doaj   +1 more source

A protocol to establish low-cost floating treatment wetlands for large-scale wastewater reclamation

STAR Protocols, 2023
Summary: Floating treatment wetlands (FTWs) consist of buoyant rafts that support the growth of macrophytes on waterbodies. The long-term performance of these rafts depends on their buoyancy and resistance to weathering.
Muhammad Arslan, Samina Iqbal, Ejazul Islam, Mohamed Gamal El-Din, Muhammad Afzal   +4 more
doaj   +1 more source

How to extract and analyze pollen from internal organs and exoskeletons of fossil insects?

STAR Protocols, 2021
Summary: This protocol explains how to extract pollen from fossil insects with subsequent descriptions of pollen treatment. We also describe how to document morphological and ultrastructural features with light-microscopy and electron microscopy.
Friðgeir Grímsson, Silvia Ulrich, Reinhard Zetter, Thomas Hörnschemeyer, Michael S. Engel, Sonja Wedmann   +5 more
doaj   +1 more source

Histone Demethylases as Counterbalance to H3K27me3 Silencing in Plants

iScience, 2020
Summary: The universal importance of epigenetic regulation has become explicit over the last decade. There is now a detailed understanding of the molecular signatures and chromatin-modifying enzymes determining epigenetic regulation.
Pedro Crevillén
doaj   +1 more source

Recent transcriptomic studies to elucidate the plant adaptive response to spaceflight and to simulated space environments

iScience, 2022
Summary: Discovering the adaptation mechanisms of plants to the space environment is essential for supporting human space exploration. Transcriptomic analyses allow the identification of adaptation response pathways by detecting changes in gene ...
Aránzazu Manzano, Eugénie Carnero-Diaz, Raúl Herranz, F. Javier Medina   +3 more
doaj   +1 more source