Results 101 to 110 of about 1,486,948 (310)

Analyzing plant stress granules in response to plant viruses [PDF]

, 2018
Plant viruses have the ability to redirect host machineries and processes to establish a productive infection. Virus-host interactions lead to the reprogramming of the plant cell cycle and transcriptional controls, inhibition of cell death pathways ...
Krenz, Björn
core  

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Manipulating plant RNA-silencing pathways to improve the gene editing efficiency of CRISPR/Cas9 systems

Genome Biology, 2018
Background The CRISPR/Cas9 system, composed of a single-guide RNA for target recognition and a Cas9 protein for DNA cleavage, has the potential to revolutionize agriculture as well as medicine. Even though extensive work has been done to improve the gene
Yanfei Mao, Xiaoxuan Yang, Yiting Zhou, Zhengjing Zhang, Jose Ramon Botella, Jian-Kang Zhu   +5 more
doaj   +1 more source

The Interpretation of Experimental Observation Data for the Development of Mechanisms based Creep Damage Constitutive Equations for High Chromium Steel [PDF]

, 2015
It is very important to design a safe factor or estimating the remain lifetime for electric power plant components of steam pipes which mostly manufacture by high chromium steels and work at high temperature and low stress level.
Lu, Zhongyu, Xu, Qiang, Yang, Xin
core   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Mechanism of floral scent production in Osmanthus fragrans and the production and regulation of its key floral constituents, β-ionone and linalool

Horticulture Research, 2019
Sweet osmanthus (Osmanthus fragrans Lour.) is among the top ten most well-known flowers in China and is recognized as both an aromatic plant and ornamental flower.
Yuanji Han, Hongyun Wang, Xiaodan Wang, Ke Li, Meifang Dong, Yong Li, Qian Zhu, Fude Shang   +7 more
doaj   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source

Application of multiple sgRNAs boosts efficiency of CRISPR/Cas9-mediated gene targeting in Arabidopsis

BMC Biology
Background Precise gene targeting (GT) is a powerful tool for heritable precision genome engineering, enabling knock-in or replacement of the endogenous sequence via homologous recombination.
Jing Li, Dali Kong, Yongping Ke, Wenjie Zeng, Daisuke Miki   +4 more
doaj   +1 more source

Investigations of the emissions of monoterpenes from Scots Pine [PDF]

, 2001
Plants produce and emit a large number of volatile organic compounds (VOC) such as isoprene and monoterpenes (C10H16). Monoterpene emission rates from Scots pine (Pinus sylvestris), a typical central European conifer, were measured under ambient ...
Komenda, Michael
core  

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source