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Neutralization Assay for SARS-CoV-2 Infection: Plaque Reduction Neutralization Test
2022Neutralization assays are often used as part of research and diagnostics to detect neutralizing antibodies and to determine a possible protective antibody titer after infection or vaccination. Here we describe a conventional plaque reduction neutralization test (PRNT) to check the presence of antibodies against SARS-CoV-2 in patient samples (serum or ...
Sandra, Westhaus, Holger F, Rabenau
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A Plaque Reduction Test for Dengue Virus Neutralizing Antibodies
The Journal of Immunology, 1967Summary A technique for performing a plaque reduction test for dengue virus neutralizing antibody is described. Experiments designed to test the accuracy of the antibody measurement are presented and analysed by the method for a parallel line graded response assay.
P K, Russell +3 more
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Dengue Virus Identification by the Plaque Reduction Neutralization Test
The Journal of Immunology, 1967Summary A method for identification and serologic classification of dengue viruses using a plaque reduction neutralization test and monkey immune serum is described. Results of typing 40 strains confirm the usefulness of this method for classification of low passage strains.
P K, Russell, A, Nisalak
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Review of diagnostic plaque reduction neutralization tests for flavivirus infection
The Veterinary Journal, 2013Flavivirus infections (including Japanese encephalitis, West Nile encephalitis and dengue fever/severe dengue) present a worldwide public health problem. Recent climate change may affect the geographical distribution of the arthropod vectors for these viruses and so the risk of flavivirus epidemics may increase. Many methods have been developed for the
Akihiko, Maeda, Junko, Maeda
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Neutralization test for bk virus: plaque reduction detected by immunoperoxidase staining
Journal of Medical Virology, 1986AbstractWe developed an immunoperoxidase staining test to detect structural antigens of BK virus (BKV) in Vero cell cultures. This test was used to examine the neutralizing activity of human and immunized animal sera. It was shown that sera positive for BKV antibodies measured by hemagglutination inhibition test and enzymelinked immunosorbent assay ...
T, Flaegstad +3 more
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Neutralization Assay for Chikungunya Virus Infection: Plaque Reduction Neutralization Test
2016Neutralization assay is a technique that detects and quantifies neutralizing antibody in serum samples by calculating the percentage of reduction of virus activity, as the concentration of virus used is usually constant. Neutralizing antibody titer is conventionally determined by calculating the percentage reduction in total virus infectivity by ...
Nor Azila Muhammad, Azami +2 more
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Guidelines for Plaque-Reduction Neutralization Testing of Human Antibodies to Dengue Viruses
Viral Immunology, 2008Through the Advisory Committee on Dengue and other Flavivirus Vaccines, the World Health Organization(WHO) has had a long-standing commitment to facilitate and to guide research and development of vaccines for medically important flaviviruses. Recently, the Paediatric Dengue Vaccine Initiative (PDVI) was formed to accelerate the development, testing ...
John T, Roehrig +2 more
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A Plaque Reduction Neutralization Test for the Detection of ZIKV-Specific Antibodies
2020Neutralizing antibodies against Zika virus (ZIKV) are a robust indicator of exposure to ZIKV. Here we describe the plaque reduction neutralization test (PRNT), which quantifies ZIKV-neutralizing antibodies (nAbs) by incubating infectious ZIKV with different dilutions of sera and applying the mixture to a susceptible Vero cell monolayer. After 4 days of
Kristina, Dimitrova +3 more
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Accuracy and repeatability of a micro plaque reduction neutralization test for vaccinia antibodies
Biologicals, 2008The detection of neutralizing antibodies against vaccinia virus is a valuable tool for the investigation of previous smallpox vaccination. Compulsory smallpox vaccination ended in Brazil during the early 1970s, although the vaccine was available until the late 1970s.
Maria Beatriz J, Borges +6 more
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Antigenic Relationships of Group a Arboviruses by Plaque Reduction Neutralization Testing
The American Journal of Tropical Medicine and Hygiene, 1975Antigenic relationships of 20 group A arboviruses were assessed by the plaque reduction neutralization test, using highly specific hyperimmune mouse ascitic fluids and antisera. The existence of three complexes of group A viruses was verified. With rare exceptions, heterologous neutralization reactions were observed only among viruses in the same ...
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