Results 91 to 100 of about 1,024,385 (225)

Electromediated formation of DNA complexes with cell membranes and its consequences for gene delivery [PDF]

arXiv, 2011
Electroporation is a physical method to induce the uptake of therapeutic drugs and DNA, by eukaryotic cells and tissues. The phenomena behind electro-mediated membrane permeabilization to plasmid DNA have been shown to be significantly more complex than those for small molecules.
arxiv  

Plasmid-Mediated Quinolone Resistance: a Multifaceted Threat

Clinical Microbiology Reviews, 2009
SUMMARY Although plasmid-mediated quinolone resistance (PMQR) was thought not to exist before its discovery in 1998, the past decade has seen an explosion of research characterizing this phenomenon.
J. Strahilevitz, G. Jacoby, D. Hooper, A. Robicsek   +3 more
semanticscholar   +1 more source

Dissemination of Cephalosporin Resistance Genes between Escherichia coli Strains from Farm Animals and Humans by Specific Plasmid Lineages

PLoS Genetics, 2014
Third-generation cephalosporins are a class of β-lactam antibiotics that are often used for the treatment of human infections caused by Gram-negative bacteria, especially Escherichia coli.
M. de Been, V. Lanza, María de Toro, J. Scharringa, W. Dohmen, Yu Du, Juan Hu, Ying Lei, Ning Li, A. Tooming-Klunderud, D. Heederik, A. Fluit, M. Bonten, R. Willems, F. de la Cruz, W. van Schaik   +15 more
semanticscholar   +1 more source

Single-molecule sequencing to track plasmid diversity of hospital-associated carbapenemase-producing Enterobacteriaceae

Science Translational Medicine, 2014
Single-molecule sequencing of bacteria at the NIH Clinical Center documents diverse plasmids encoding antibiotic resistance and their transfer between microbes.
S. Conlan, Pamela J. Thomas, C. Deming, Morgan Park, A. Lau, J. Dekker, E. Snitkin, Tyson A. Clark, K. Luong, Yi Song, Y. Tsai, Matthew Boitano, J. Dayal, Shelise Y. Brooks, B. Schmidt, Alice C. Young, James W. Thomas, G. Bouffard, R. Blakesley, J. Mullikin, J. Korlach, D. Henderson, K. Frank, T. Palmore, J. Segre   +24 more
semanticscholar   +1 more source

Predictable, Tunable Protein Production in Salmonella for Studying Host-Pathogen Interactions

Frontiers in Cellular and Infection Microbiology, 2017
Here we describe the use of synthetic genetic elements to improve the predictability and tunability of episomal protein production in Salmonella. We used a multi-pronged approach, in which a series of variable-strength synthetic promoters were combined ...
Kendal G. Cooper, Audrey Chong, Tregei Starr, Ciaran E. Finn, Olivia Steele-Mortimer   +4 more
doaj   +1 more source

Dominance of the Inducible State in Strains of Staphylococcus aureus Containing Two Distinct Penicillinase Plasmids [PDF]

, 1965
M. H. Richmond
openalex   +1 more source

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA

Scientific Reports, 2013
CRISPR/Cas mediated genome editing has been successfully demonstrated in mammalian cells and further applications for generating mutant mice were reported by injecting humanized Cas9 (hCas) mRNA and single guide RNA into fertilized eggs.
Daisuke Mashiko, Yoshitaka Fujihara, Yuhkoh Satouh, H. Miyata, A. Isotani, M. Ikawa   +5 more
semanticscholar   +1 more source

Surface charges effects on the 2D conformation of supercoiled DNA [PDF]

arXiv, 2012
We have adsorbed plasmid PuC19 DNA on a supported bilayer. The mobility of the lipids within the bilayer ensured a 2D equilibrium of the DNA molecule. By varying the fraction of cationic lipids in the membrane, we have tuned the surface charge. Plasmids conformations were imaged by Atomic Force Microscopy (AFM).We performed two sets of experiments ...
arxiv  

Corrigendum: First Report of Coexistence of blaSFO-1 and blaNDM-1 β-Lactamase Genes as Well as Colistin Resistance Gene mcr-9 in a Transferrable Plasmid of a Clinical Isolate of Enterobacter hormaechei

Frontiers in Microbiology, 2021
Wenxiu Ai, Ying Zhou, Ying Zhou, Bingjie Wang, Bingjie Wang, Qing Zhan, Qing Zhan, Longhua Hu, Yanlei Xu, Yanlei Xu, Yinjuan Guo, Yinjuan Guo, Liangxing Wang, Fangyou Yu, Fangyou Yu, Xiaolong Li   +15 more
doaj   +1 more source

Antimicrobial Resistance Patterns and Plasmid Profiles of Methicillin Resistant Staphylococcus aureus Isolated from Clinical Samples

Nepal Journal of Biotechnology, 2019
Methicillin-resistant Staphylococcus aureus (MRSA), showing resistance to several antibiotics is a global health problem associated with considerable mortality and morbidity.
Gaurav Agrahari, Amrit Koirala, Roshan Thapa, Mahesh Kumar Chaudhary, Reshma Tuladhar   +4 more
doaj   +1 more source