Results 161 to 170 of about 1,390,499 (412)
Structure of a translocation signal domain mediating conjugative transfer by Type IV secretion systems [PDF]
, 2013 Relaxases are proteins responsible for the transfer of plasmid and chromosomal DNA from one bacterium to another during conjugation. They covalently react with a specific phosphodiester bond within DNA origin of transfer sequences, forming a nucleo ...
Gruber, C.J. +7 more
core +1 more source
PLoS Genetics, 2014 Third-generation cephalosporins are a class of β-lactam antibiotics that are often used for the treatment of human infections caused by Gram-negative bacteria, especially Escherichia coli.
M. de Been +15 more
semanticscholar +1 more source
FEBS Open Bio, EarlyView.Heterologous expression of membrane transporters in cultured cells is essential for functional characterization, but is sometimes limited by low activity. Our study compares the HDAC inhibitors butyrate, VPA and SAHA to enhance transport activity. We propose to replace butyrate by SAHA: it is equally effective, devoid of repulsive odor, costs less, and
Svenja Flögel +4 more
wiley +1 more source
Science Translational Medicine, 2014 Single-molecule sequencing of bacteria at the NIH Clinical Center documents diverse plasmids encoding antibiotic resistance and their transfer between microbes.
S. Conlan +24 more
semanticscholar +1 more source
FEBS Open Bio, EarlyView.SLC7A11 frequently migrates faster in SDS‐PAGE. The present study found that the high hydrophobicity of SLC7A11 causes its anomalous migration in SDS‐PAGE with a low concentration of acrylamide gel. Replacing isoleucine with asparagine reduced hydrophobicity and restored its normal migration at 55 kDa, revealing the role of hydrophobicity and gel ...
Nsengiyumva Emmanuel +13 more
wiley +1 more source
Predictable, Tunable Protein Production in Salmonella for Studying Host-Pathogen Interactions
Frontiers in Cellular and Infection Microbiology, 2017 Here we describe the use of synthetic genetic elements to improve the predictability and tunability of episomal protein production in Salmonella. We used a multi-pronged approach, in which a series of variable-strength synthetic promoters were combined ...
Kendal G. Cooper +4 more
doaj +1 more source
Surface charges effects on the 2D conformation of supercoiled DNA [PDF]
arXiv, 2012 We have adsorbed plasmid PuC19 DNA on a supported bilayer. The mobility of the lipids within the bilayer ensured a 2D equilibrium of the DNA molecule. By varying the fraction of cationic lipids in the membrane, we have tuned the surface charge. Plasmids conformations were imaged by Atomic Force Microscopy (AFM).We performed two sets of experiments ...
arxiv
Scientific Reports, 2013 CRISPR/Cas mediated genome editing has been successfully demonstrated in mammalian cells and further applications for generating mutant mice were reported by injecting humanized Cas9 (hCas) mRNA and single guide RNA into fertilized eggs.
Daisuke Mashiko +5 more
semanticscholar +1 more source
FEBS Open Bio, EarlyView.Liver‐specific knockout of N6‐methyladenosine (m6A) methyltransferase METTL3 significantly accelerated hepatic tumor initiation under various oncogenic challenges, contrary to the previously reported oncogenic role of METTL3 in liver cancer cell lines or xenograft models. Mechanistically, METTL3 deficiency reduced m6A deposition on Manf transcripts and
Bo Cui +15 more
wiley +1 more source
How the other half lives: CRISPR-Cas's influence on bacteriophages [PDF]
Evolutionary Biology : Self, Non-Self Evolution, Species and
Complex Traits, Evolution, Methods and Concepts, ISBN 978-3-319-61569-1,
edited by Pierre Pontarotti, Springer Nature, September 2017, pp. 63-85, 2017 CRISPR-Cas is a genetic adaptive immune system unique to prokaryotic cells used to combat phage and plasmid threats. The host cell adapts by incorporating DNA sequences from invading phages or plasmids into its CRISPR locus as spacers. These spacers are expressed as mobile surveillance RNAs that direct CRISPR-associated (Cas) proteins to protect ...
arxiv