Results 231 to 240 of about 1,232,660 (311)

The development of a methodology to measure primary ciliary length in neural progenitor cells with a target molecule knocked down. [PDF]

Biochem Biophys Rep
Saito M, Otsu W, Ishibashi K, Atsumi GI.
europepmc   +1 more source

Attenuation of Influenza a Virus into Live Vaccines Through C‐End Degrons

Advanced Science, EarlyView.
Naturally occurring C‐end degrons are exploited to design proteolysis‐targeting live attenuated influenza vaccines. C‐end degron tagging of viral M1 protein promotes proteasome‐dependent degradation, resulting in robust attenuation in host cells while permitting scalable production in engineered cells.
Ping Wang, Le Li, Yunfang Chen, Le Tong, Zhen Li, Rong Yu, Quan Shen, Qikai Wang, Jihuan Hou, Qisi Zhang, Xu Si, Ning Wang, Demin Zhou, Wen‐xia Tian, Longlong Si   +14 more
wiley   +1 more source

UniClo technology exploits methylation for universal scarless DNA assembly. [PDF]

Biol Methods Protoc
Flores-Fernández CN, Lin D, Robins K, O'Callaghan CA.   +3 more
europepmc   +1 more source

A Novel Function of Nonadecanoic Acid in Regulating Glucose Homeostasis

Advanced Science, EarlyView.
This study identifies the odd‐chain fatty acid C19:0 as an endogenous ligand of GPR120 that promotes glucose homeostasis via Gαq signaling. In obesity, elevated palmitic acid suppresses endogenous C19:0 production through a miRNA–PPARα–HACL1 pathway, offering a promising direction for understanding the role of OCFAs in human health.
Yanting Hou, Yinghua Ma, Qin Liu, Dingling Ma, Yuxin Tong, Lili Xu, Xiaolong Chu, Jingzhou Wang, Maodi Liang, Mengyuan Zhao, Huizi Zhang, Yurui Su, Jianxin Xie, Cuizhe Wang, Jun Zhang   +14 more
wiley   +1 more source

CtGAP 2.0: enhanced genome assembly, strain resolution, and phylogenomics for <i>Chlamydia trachomatis</i>. [PDF]

Microbiol Resour Announc
Olagoke O, Read TD, Dean D.
europepmc   +1 more source

Deoxyribonucleic ccid synthesis in isolated chloroplasts and chloroplast extracts of maize [PDF]

, 1982
Zimmermann, Wolfgang
core  

Testicular mRNA‐LNP Delivery: A Novel Therapy for Genetic Spermatogenic Disorders

Advanced Science, EarlyView.
In the current study, we developed a novel therapy for genetic spermatogenic disorders. It was revealed that in vivo delivery of mRNA LNP3 could restore spermatogenesis in Msh5D486Y/D486Y and Maps KO mouse models with meiotic arrest. Notably, the offspring without genomic integration was born using ICSI derived from the rescue of Msh5D486Y/D486Y mouse ...
Chenwang Zhang, Nan Liang, Wenbo Li, Shuai Xu, Peng Li, Wanze Ni, Na Li, Sha Han, Ningjing Ou, Haowei Bai, Yuxiang Zhang, Furong Bai, Yifan Sun, Dewei Qian, Xinjie Bu, Erlei Zhi, Ruhui Tian, Yuhua Huang, Jingpeng Zhao, Fujun Zhao, Hao Chen, Zheng Li, Chencheng Yao   +22 more
wiley   +1 more source

Plasmid mutation rates scale with copy number. [PDF]

Proc Natl Acad Sci U S A
Ramiro-Martínez P, Quinto I, Jaraba-Soto L, Lanza VF, Herencias-Rodríguez C, González Casanova A, Peña-Miller R, Rodríguez-Beltrán J.   +7 more
europepmc   +1 more source

Comprehensive Profiling of N6‐methyladnosine (m6A) Readouts Reveals Novel m6A Readers That Regulate Human Embryonic Stem Cell Differentiation

Advanced Science, EarlyView.
This research deciphers the m6A transcriptome by profiling its sites and functional readout effects: from mRNA stability, translation to alternative splicing, across five different cell types. Machine learning model identifies novel m6A‐binding proteins DDX6 and FXR2 and novel m6A reader proteins FUBP3 and L1TD1.
Zhou Huang, Rucong Liu, Zibaguli Wubulikasimu, Wanqing Zhao, Jiaqi Huang, Jiaxuan Wang, Tianyuan Zhang, Rui Fan, Wei Kong, Qinghua Cui, Yang Li, Yuan Zhou   +11 more
wiley   +1 more source

Erratum to "<i>CX3CR1</i> ⁺ Monocytes/Macrophages Promote Regional Immune Injury in Mesangial Proliferative Glomerulonephritis through Crosstalk with Activated Mesangial Cells". [PDF]

Research (Wash D C)
Zhang J, Fang Q, Huang Y, Qu Y, Liu Q, Li R, Zhou Y, Cui S, Liu R, Wang X, Bai Y, Duan S, Wu L, Chen P, Wang Y, Wu J, Sun X, Cai G, Zheng Y, Hong Q, Chen X.   +20 more
europepmc   +1 more source