Results 51 to 60 of about 1,449,118 (412)
Evidence that the 36kb plasmid of Brachyspira hyodysenteriae contributes to virulence [PDF]
Swine dysentery (SD) results from infection of the porcine large intestine with the anaerobic intestinal spirochaete Brachyspira hyodysenteriae. Recently the genome of virulent Australian B. hyodysenteriae strain WA1 was sequenced, and a 36. kilobase (kb)
Bellgard, M.I.+5 more
core +2 more sources
Antiparallel plasmid-plasmid pairing may control P1 plasmid replication. [PDF]
The copy number of the P1 plasmid replicon is stringently controlled, giving only one or two copies per newborn cell. Control is achieved by the action of the copy-control locus incA, which contains nine repeats of the 19-basepair binding site for the plasmid-encoded initiator protein RepA.
Ann L. Abeles, Stuart Austin
openaire +3 more sources
Noise enhanced persistence in a biochemical regulatory network with feedback control [PDF]
We find that discrete noise of inhibiting (signal) molecules can greatly delay the extinction of plasmids in a plasmid replication system: a prototypical biochemical regulatory network. We calculate the probability distribution of the metastable state of the plasmids and show on this example that the reaction rate equations may fail in predicting the ...
arxiv +1 more source
Determination of plasmid copy number in yeast transformants by means of agarose plugs [PDF]
The determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection.
AM Guerrini+3 more
core +1 more source
Plasmids in Rhizobia: The Role of Nonsymbiotic Plasmids
Peer ...
Mercado-Blanco, Jesús, Toro, Nicolás
openaire +3 more sources
Predictive Modeling of Non-Viral Gene Transfer [PDF]
In non-viral gene delivery, the variance of transgenic expression stems from the low number of plasmids successfully transferred. Here, we experimentally determine Lipofectamine- and PEI-mediated exogenous gene expression distributions from single cell time-lapse analysis.
arxiv +1 more source
Exploiting CRISPR-Cas to manipulate Enterococcus faecalis populations
CRISPR-Cas provides a barrier to horizontal gene transfer in prokaryotes. It was previously observed that functional CRISPR-Cas systems are absent from multidrug-resistant (MDR) Enterococcus faecalis, which only possess an orphan CRISPR locus, termed ...
Karthik Hullahalli+2 more
doaj +1 more source
Klebsiella pneumoniae exhibits extensive phenotypic and genetic diversity. Higher plasmid loads in the cell were supposed to play an key role in its genome diversity.
Mufeng Cai+23 more
doaj +1 more source
Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review
Carbapenem‐resistant Enterobacteriaceae (CRE) have been listed by the WHO as high‐priority pathogens owing to their high association with mortalities and morbidities.
K. Kopotsa, John Osei Sekyere, N. Mbelle
semanticscholar +1 more source
Guidelines for the estimation and reporting of plasmid conjugation rates [PDF]
Conjugation is a central characteristic of plasmid biology and an important mechanism of horizontal gene transfer in bacteria. However, there is little consensus on how to accurately estimate and report plasmid conjugation rates, in part due to the wide range of available methods.
arxiv