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INTRODUCTION: non-typhoidal Salmonella is the causative agent of gastroenteritis, a food-borne and zoonotic infection which is a major cause of high morbidity and death among children under 5 years of age especially from resource poor settings like the ...
Ajoke Olutola Adagbada+3 more
doaj +1 more source
Determination of plasmid copy number in yeast transformants by means of agarose plugs [PDF]
The determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection.
AM Guerrini+3 more
core +1 more source
Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes blinding trachoma and sexually transmitted disease. C. trachomatis isolates are classified into 2 biovars—lymphogranuloma venereum (LGV) and trachoma—which are ...
Michael John Patton+12 more
doaj +1 more source
Guidelines for the estimation and reporting of plasmid conjugation rates [PDF]
Conjugation is a central characteristic of plasmid biology and an important mechanism of horizontal gene transfer in bacteria. However, there is little consensus on how to accurately estimate and report plasmid conjugation rates, in part due to the wide range of available methods.
arxiv
Plasmid evolution in carbapenemase‐producing Enterobacteriaceae: a review
Carbapenem‐resistant Enterobacteriaceae (CRE) have been listed by the WHO as high‐priority pathogens owing to their high association with mortalities and morbidities.
K. Kopotsa, John Osei Sekyere, N. Mbelle
semanticscholar +1 more source
The clinical spectrum of SMA‐PME and in vitro normalization of its cellular ceramide profile
Abstract Objective The objectives of this study were to define the clinical and biochemical spectrum of spinal muscular atrophy with progressive myoclonic epilepsy (SMA‐PME) and to determine if aberrant cellular ceramide accumulation could be normalized by enzyme replacement.
Michelle M. Lee+16 more
wiley +1 more source
Role of AcrAB-TolC multidrug efflux pump in drug-resistance acquisition by plasmid transfer
A race against time Clinically relevant antimicrobial resistance is largely spread via plasmids that disperse among bacteria during conjugation. How quickly can a resistance gene be expressed after transfer?
S. Nolivos+5 more
semanticscholar +1 more source
Extended-spectrum cephalosporin-resistant (ESCR) Enterobacteriaceae pose a serious infection control challenge for public health. The emergence of the ESCR phenotype is mostly facilitated by plasmid-mediated horizontal extended-spectrum β-lactamases ...
Apostolos Liakopoulos+10 more
doaj +1 more source
Intracellular Delivery of DNA and Protein by a Novel Cell-Permeable Peptide Derived from DOT1L
Cellular uptake and intracellular release efficiency of biomacromolecules is low because of hurdles in the cell membrane that result in limited access to intra-cellular targets with few functional effects.
Jingping Geng+6 more
doaj +1 more source
First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus
We describe the identification of a methicillin-resistant, high-level mupirocin-resistant Staphylococcus argenteus. The isolate (1801221) was characterized as t6675-ST2250-SCCmecIVc, and whole-genome sequencing revealed that the isolate possessed two ...
Adebayo Osagie Shittu+7 more
doaj +1 more source