Results 31 to 40 of about 842,240 (269)

Enhancing student understanding of genetic cloning through a 3D-printed active learning activity and flipped classroom curriculum

Journal of Microbiology & Biology Education
This classroom curriculum is designed to teach the topic of cloning in both small- and large-enrollment undergraduate general microbiology courses. Students often struggle to grasp and visualize genetic engineering or cloning, in which a piece of DNA is ...
Katriana A. Popichak, Kathryn Krupinsky, Delaney Worthington, Samuel Fredricks, Leila Takahashi-Ruiz, Mary Howerton, Marcela Henao-Tamayo, Erica L. Suchman, Jennifer L. McLean   +8 more
doaj   +1 more source

Characterization of a novel Phietavirus genus bacteriophage and its potential for efficient transfer of modified shuttle plasmids to Staphylococcus aureus strains of different clonal complexes

Microbiology Spectrum
Staphylococcus aureus is a significant human pathogen responsible for various nosocomial and community-acquired infections, leading to considerable morbidity and mortality worldwide. Temperate bacteriophages contribute to its virulence and facilitate the
Łukasz Kałuski, Emil Stefańczyk, Aleksandra Głowacka-Rutkowska, Jan Gawor, Joanna Empel, Monika Orczykowska-Kotyna, Aleksandra Szczypkowska, Karolina Żuchniewicz, Robert Gromadka, Małgorzata Łobocka   +9 more
doaj   +1 more source

KPC-2-Producing Carbapenem-Resistant Klebsiella pneumoniae of the Uncommon ST29 Type Carrying OXA-926, a Novel Narrow-Spectrum OXA β-Lactamase

Frontiers in Microbiology, 2021
We isolated and characterized a carbapenem-resistant Klebsiella pneumoniae (CRKP) clinical strain from blood carrying a novel blaOXA gene, blaOXA–926, and belonging to ST29, an uncommon CRKP type. The strain, 130002, was genome sequenced using both short-
Lina Liu, Yu Feng, Li Wei, Yuling Xiao, Zhiyong Zong, Zhiyong Zong, Zhiyong Zong, Zhiyong Zong   +7 more
doaj   +1 more source

Genetic Insights on Meropenem Resistance Concerning Klebsiella pneumoniae Clinical Isolates

Life
The transferable genetic elements are associated with the dissemination of virulence determinants amongst Klebsiella pneumoniae. Thus, we assessed the correlated antimicrobial resistance in carbapenem-resistant Klebsiella pneumoniae clinical isolates ...
Fathy M. Elkady, Bahaa M. Badr, Abdel-Aty E. Alfeky, Mohammed S. Abdulrahman, Amr H. Hashem, Abdulaziz A. Al-Askar, Gehad AbdElgayed, Hany R. Hashem   +7 more
doaj   +1 more source

A Primary Physiological Role of Toxin/Antitoxin Systems Is Phage Inhibition

Frontiers in Microbiology, 2020
Toxin/antitoxin (TA) systems are present in most prokaryote genomes. Toxins are almost exclusively proteins that reduce metabolism (but do not cause cell death), and antitoxins are either RNA or proteins that counteract the toxin or the RNA that encodes ...
Sooyeon Song, Thomas K. Wood
doaj   +1 more source

First Report of a Methicillin-Resistant, High-Level Mupirocin-Resistant Staphylococcus argenteus

Frontiers in Cellular and Infection Microbiology, 2022
We describe the identification of a methicillin-resistant, high-level mupirocin-resistant Staphylococcus argenteus. The isolate (1801221) was characterized as t6675-ST2250-SCCmecIVc, and whole-genome sequencing revealed that the isolate possessed two ...
Adebayo Osagie Shittu, Adebayo Osagie Shittu, Franziska Layer-Nicolaou, Birgit Strommenger, Minh-Thu Nguyen, Stefan Bletz, Alexander Mellmann, Frieder Schaumburg   +7 more
doaj   +1 more source

Enteropathogenic E. coli shows delayed attachment and host response in human jejunum organoid‐derived monolayers compared to HeLa cells

FEBS Letters, EarlyView.
Enteropathogenic E. coli (EPEC) infects the human intestinal epithelium, resulting in severe illness and diarrhoea. In this study, we compared the infection of cancer‐derived cell lines with human organoid‐derived models of the small intestine. We observed a delayed in attachment, inflammation and cell death on primary cells, indicating that host ...
Mastura Neyazi, Pilar Samperio Ventayol, Natalie Burkard, Nicolas Schlegel, Carmen Aguilar, Sina Bartfeld   +5 more
wiley   +1 more source

Intramuscular DNA vaccine provides protection in non-human primate and mouse models of SARS-CoV-2

Frontiers in Immunology
Nucleic acid vaccine approaches have proven successful in the context of the SARS-CoV-2 pandemic, however challenges with delivery remain. Here we describe PlaCCine, a DNA-based vaccine platform that utilizes a device- and vector-free chemical delivery ...
Subeena Sood, Ebony N. Gary, Majed Matar, Jessica Kim, Casey E. Hojecki, Bryce Warner, Robert Vendramelli, Thang Truong, Alanna Smith, Jennifer Rice, Jeff Sparks, Michael DeSalvo, John Henderson, Joseph A. Rogers, Ankur Sharma, Laurent Pessaint, Carlo A. Iavarone, Darwyn Kobasa, Jean D. Boyer, Stacy Lindborg, Khursheed Anwer   +20 more
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source