Results 21 to 30 of about 1,194,207 (363)

DNA Minicircle Technology Improves Purity of Adeno-associated Viral Vector Preparations

Molecular Therapy: Nucleic Acids, 2016
Adeno-associated viral (AAV) vectors are considered as one of the most promising delivery systems in human gene therapy. In addition, AAV vectors are frequently applied tools in preclinical and basic research. Despite this success, manufacturing pure AAV
Maria Schnödt, Marco Schmeer, Barbara Kracher, Christa Krüsemann, Laura Escalona Espinosa, Anja Grünert, Thomas Fuchsluger, Anja Rischmüller, Martin Schleef, Hildegard Büning   +9 more
doaj   +1 more source

Prevalence of bla_KPC-2, bla_KPC-3 and bla_KPC-30—Carrying Plasmids in Klebsiella pneumoniae Isolated in a Brazilian Hospital

Pathogens, 2021
Klebsiella pneumoniae carbapenemase (KPC) actively hydrolyzes carbapenems, antibiotics often used a last-line treatment for multidrug-resistant bacteria. KPC clinical relevance resides in its widespread dissemination.
Letícia B. Migliorini, Romário O. de Sales, Paula C. M. Koga, Andre M. Doi, Anja Poehlein, Alexandra R. Toniolo, Fernando G. Menezes, Marines D. V. Martino, Ana C. Gales, Holger Brüggemann, Patricia Severino   +10 more
doaj   +1 more source

Phenotypic and Genotypic Properties of Fluoroquinolone-Resistant, qnr-Carrying Escherichia coli Isolated from the German Food Chain in 2017

Microorganisms, 2021
Fluoroquinolones are the highest priority, critically important antimicrobial agents. Resistance development can occur via different mechanisms, with plasmid-mediated quinolone resistance (PMQR) being prevalent in the livestock and food area. Especially,
Katharina Juraschek, Carlus Deneke, Silvia Schmoger, Mirjam Grobbel, Burkhard Malorny, Annemarie Käsbohrer, Stefan Schwarz, Diana Meemken, Jens Andre Hammerl   +8 more
doaj   +1 more source

Calcium-responsive plasmid copy number regulation is dependent on discrete YopD domains in Yersinia pseudotuberculosis

, 2023
Yersinia pathogenicity depends mainly on a Type III Secretion System (T3SS) responsible for translocating effector proteins into the eukaryotic target cell cytosol. The T3SS is encoded on a 70 kb, low copy number virulence plasmid, pYV.
Wang, Helen, Engling, Pit,, Tifaine Héchard, Edgren, Tomas,, Engling, Pit, Tomas Edgren, Francis, Matthew,, Francis, Matthew, Pit Engling, Héchard, Tifaine, Edgren, Tomas, Dersch, Petra,, Wang, Helen,, Héchard, Tifaine,, Helen Wang, Dersch, Petra, Matthew Francis, Petra Dersch   +17 more
core   +1 more source

High-Yield Expression and Purification of Recombinant Influenza Virus Proteins from Stably-Transfected Mammalian Cell Lines

Vaccines, 2020
Influenza viruses infect millions of people each year, resulting in significant morbidity and mortality in the human population. Therefore, generation of a universal influenza virus vaccine is an urgent need and would greatly benefit public health ...
Jeffrey W. Ecker, Greg A. Kirchenbaum, Spencer R. Pierce, Amanda L. Skarlupka, Rodrigo B. Abreu, R. Ethan Cooper, Dawn Taylor-Mulneix, Ted M. Ross, Giuseppe A. Sautto   +8 more
doaj   +1 more source

Plasmid-Mediated Antibiotic Resistant Escherichia coli in Sarawak Rivers and Aquaculture Farms, Northwest of Borneo

Antibiotics, 2021
Background: The emergence of plasmid-mediated antibiotic resistance in Escherichia coli in water resources could pose a serious threat to public health. The study aims to investigate the dispersion of plasmid-mediated antibiotic-resistant E.
Samuel Lihan, Sai Y. Lee, Seng C. Toh, Sui S. Leong   +3 more
doaj   +1 more source

Iteron Plasmids

Microbiology Spectrum, 2014
ABSTRACT Iteron-containing plasmids are model systems for studying the metabolism of extrachromosomal genetic elements in bacterial cells. Here we describe the current knowledge and understanding of the structure of iteron-containing replicons, the structure of the iteron plasmid encoded replication initiation proteins, and the molecular ...
Igor, Konieczny, Katarzyna, Bury, Aleksandra, Wawrzycka, Katarzyna, Wegrzyn   +3 more
openaire   +2 more sources

Multiple pathways of plasmid DNA transfer in Helicobacter pylori [PDF]

, 2012
Many Helicobacter pylori (Hp) strains carry cryptic plasmids of different size and gene content, the function of which is not well understood. A subgroup of these plasmids (e.g.
Haas, Rainer, Rainer Haas, Lea Holsten (133951), Wolfgang Fischer (133965), Holsten, Lea, Stefanie Rohrer (133947), Rohrer, Stefanie, Rainer Haas (81562), Weiss, Evelyn, Lea Holsten, Mohammed Benghezal, Evelyn Weiss (133955), Mohammed Benghezal (133961), Stefanie Rohrer, Wolfgang Fischer, Benghezal, Mohammed, Evelyn Weiss, Fischer, Wolfgang   +17 more
core   +1 more source

Plasmid classifications

Plasmid, 2023
Plasmids are universally present in bacteria and play key roles in the dissemination of genes such as antibiotic resistance determinants. Major concepts in Plasmid Biology derive from the efforts to classify plasmids. Here, we review the main plasmid classification systems, starting by phenotype-based methods, such as fertility inhibition and ...
Garcillán Barcia, María del Pilar, Redondo Salvo, Santiago, Cruz, Fernando de la   +2 more
openaire   +4 more sources

Universal whole-sequence-based plasmid typing and its utility to prediction of host range and epidemiological surveillance

Microbial Genomics, 2020
Bacterial plasmids play a large role in allowing bacteria to adapt to changing environments and can pose a significant risk to human health if they confer virulence and antimicrobial resistance (AMR).
James Robertson, K. Bessonov, J. Schonfeld, John H. E. Nash   +3 more
semanticscholar   +1 more source