Results 21 to 30 of about 1,239,164 (310)

Determination of plasmid copy number in yeast transformants by means of agarose plugs [PDF]

open access: yes, 1993
The determination of plasmid copy number in Saccharomyces cerevisiaetransformants containing circular or linear plasmids is currently performed with total yeast DNA extracts obtained from cultures grown under selection.
AM Guerrini   +3 more
core   +2 more sources

On the isolation of TI-plasmid from Agrobacterium tumefaciens [PDF]

open access: yes, 1976
An efficient lysis method for Agrobacterium cells was developed, which allows a reproducible isolation of the tumor inducing (TI)-plasmid. The lysis method is based on the sensitivity of this bacterium to incubation with lysozyme, n-dodecylamine,EDTA ...
Dons, Johannes JM   +7 more
core   +2 more sources

Comparison of the effectiveness of chlorine, ozone, and photocatalytic disinfection in reducing the risk of antibiotic resistance pollution [PDF]

open access: yes, 2011
Effectiveness of conventional chlorine and ozone disinfection on reduction of antibiotic resistance was compared with less commonly applied heterogeneous photocatalytic process.
Balcioglu, Isil Akmehmet   +5 more
core   +1 more source

Plasmid Negative Regulation of CPAF Expression Is Pgp4 Independent and Restricted to Invasive Chlamydia trachomatis Biovars

open access: yesmBio, 2018
Chlamydia trachomatis is an obligate intracellular bacterial pathogen that causes blinding trachoma and sexually transmitted disease. C. trachomatis isolates are classified into 2 biovars—lymphogranuloma venereum (LGV) and trachoma—which are ...
Michael John Patton   +12 more
doaj   +1 more source

Co-occurrence of blaNDM–1 and mcr-9 in a Conjugative IncHI2/HI2A Plasmid From a Bloodstream Infection-Causing Carbapenem-Resistant Klebsiella pneumoniae

open access: yesFrontiers in Microbiology, 2021
Spread of the carbapenemase-encoding and mobilized colistin resistance (mcr) genes among Enterobacteriales poses a great threat to global public health, especially when the both genes are transferred by a single plasmid.
Zhou Liu   +13 more
doaj   +1 more source

Expression of Green Fluorescence Protein (GFP) in Zebrafish Muscle through Injection: A Gene Therapy Model [PDF]

open access: yes, 2008
Expression of the target gene is important for gene therapy. Presently, localized transgenesis is used for gene therapy which can be achieved by a target gene expression. Here, we have reported the plasmid mediated gene therapy to zebrafish model.
C. H. Hsu   +4 more
core   +2 more sources

Evidence that the 36kb plasmid of Brachyspira hyodysenteriae contributes to virulence [PDF]

open access: yes, 2011
Swine dysentery (SD) results from infection of the porcine large intestine with the anaerobic intestinal spirochaete Brachyspira hyodysenteriae. Recently the genome of virulent Australian B. hyodysenteriae strain WA1 was sequenced, and a 36. kilobase (kb)
Bellgard, M.I.   +5 more
core   +2 more sources

Multiple pathways of plasmid DNA transfer in Helicobacter pylori [PDF]

open access: yes, 2012
Many Helicobacter pylori (Hp) strains carry cryptic plasmids of different size and gene content, the function of which is not well understood. A subgroup of these plasmids (e.g.
Benghezal, Mohammed   +5 more
core   +3 more sources

Genome characteristics of the optrA-positive Clostridium perfringens strain QHY-2 carrying a novel plasmid type

open access: yesmSystems, 2023
Clostridium perfringens is a bacterial species of importance to both public and animal health. The gene optrA is the first gene that confers resistance to the tedizolid, a last-resort antimicrobial agent in human medicine.
Ke Wu   +15 more
doaj   +1 more source

Antiparallel plasmid-plasmid pairing may control P1 plasmid replication. [PDF]

open access: yesProceedings of the National Academy of Sciences, 1991
The copy number of the P1 plasmid replicon is stringently controlled, giving only one or two copies per newborn cell. Control is achieved by the action of the copy-control locus incA, which contains nine repeats of the 19-basepair binding site for the plasmid-encoded initiator protein RepA.
A L, Abeles, S J, Austin
openaire   +2 more sources

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