Results 51 to 60 of about 145,569 (114)

Subinhibitory Concentration of Colistin Promotes the Conjugation Frequencies of Mcr-1- and blaNDM-5-Positive Plasmids

Microbiology Spectrum, 2022
Horizontal gene transfer (HGT) plays a significant role in the spread of antibiotic resistance genes (ARGs). Most reported compounds promote HGT by increasing the cell membrane permeability.
Xia Xiao, Fuxin Zeng, Ruichao Li, Yuan Liu, Zhiqiang Wang   +4 more
doaj   +1 more source

The evolution of plasmid-carried antibiotic resistance

BMC Evolutionary Biology, 2011
Background Antibiotic resistance represents a significant public health problem. When resistance genes are mobile, being carried on plasmids or phages, their spread can be greatly accelerated.
Rankin Daniel J, Svara Fabian
doaj   +1 more source

A High-Throughput Method for Screening for Genes Controlling Bacterial Conjugation of Antibiotic Resistance

mSystems, 2020
The rapid horizontal transmission of antibiotic resistance genes on conjugative plasmids between bacterial host cells is a major cause of the accelerating antibiotic resistance crisis.
Hanna Alalam, Fabrice E. Graf, Martin Palm, Marie Abadikhah, Martin Zackrisson, Jonas Boström, Alfred Fransson, Chris Hadjineophytou, Linnéa Persson, Simon Stenberg, Matilda Mattsson, Payam Ghiaci, Per Sunnerhagen, Jonas Warringer, Anne Farewell   +14 more
doaj   +1 more source

Structure of a translocation signal domain mediating conjugative transfer by Type IV secretion systems [PDF]

, 2013
Relaxases are proteins responsible for the transfer of plasmid and chromosomal DNA from one bacterium to another during conjugation. They covalently react with a specific phosphodiester bond within DNA origin of transfer sequences, forming a nucleo ...
Redzej, Adam, Topf, Maya, Zechner, E.L., Lang, S., Waksman, Gabriel, Zangger, K., Gruber, C.J., Ilangovan, Aravindan   +7 more
core   +1 more source

Sequence analysis of a cryptic plasmid pCJ419 from Campylobacter jejuni and construction of an Escherichia coli-Campylobacter shuttle vector.

, 2003
A small cryptic plasmid, pCJ419, was identified in a human clinical isolate of Campylobacter jejuni, cloned and sequenced. pCJ419 is a circular molecule of 4013 bp with a G + C content of 27.1%.
Alfredson, DA, Korolik, V
core   +1 more source

Biology of the staphylococcal conjugative multiresistance plasmid pSK41

, 2013
Plasmid pSK41 is a large, low-copy-number, conjugative plasmid from Staphylococcus aureus that is representative of a family of staphylococcal plasmids that confer multiple resistances to a wide range of antimicrobial agents.
Jensen, Slade O. (R16034), Liu, Michael A., Firth, Neville, Kwong, Stephen M. (R20881), Brzoska, Anthony J.   +4 more
core   +1 more source

A convenient method to generate and maintain poly(A)-encoding DNA sequences required for in vitro transcription of mRNA

BioTechniques, 2019
Generating mRNA in vitro to encode therapeutic or cell-modifying proteins is rapidly gaining favor. An important factor that determines efficiency of translation from in vitro transcribed mRNA is the length of the 3′ poly(A) sequence.
Patrick Arbuthnot, Abdullah Ely, Kristie Bloom   +2 more
doaj   +1 more source

Monomeric PcrA helicase processively unwinds plasmid lengths of DNA in the presence of the initiator protein RepD [PDF]

, 2013
The helicase PcrA unwinds DNA during asymmetric replication of plasmids, acting with an initiator protein, in our case RepD. Detailed kinetics of PcrA activity were measured using bulk solution and a single-molecule imaging technique to investigate the ...
Fili, Natalia, Webb, Martin R, Mashanov, Gregory I, Chisty, Liisa T, Toseland, Christopher P., Dillingham, Mark S, Toseland, Christopher P, Molloy, Justin E   +7 more
core   +1 more source

Adaptative Potential of the Lactococcus Lactis IL594 Strain Encoded in Its 7 Plasmids [PDF]

, 2011
The extrachromosomal gene pool plays a significant role both in evolution and in the environmental adaptation of bacteria. The L. lactis subsp. lactis IL594 strain contains seven plasmids, named pIL1 to pIL7, and is the parental strain of the plasmid ...
Koryszewska-Bagińska, Anna, Bardowski, Jacek K., Grynberg, Marcin, Gołębiewski, Marcin, Żylińska, Joanna, Górecki, Roman Krzysztof   +5 more
core  

Mapping of the nick site on conjugative plasmid pVT745 by interrupted mating [PDF]

, 2010
Conjugal transfer of plasmid DNA initiates and terminates at a specific non-coding site called the origin of transfer (oriT). Previous analysis of conjugative plasmid pVT745 from Aggregatibacter actinomycetemcomitans suggested that oriT was located ...
Pappas, Donald L., Galli, Dominique M., Chen, Jinbiao   +2 more
core   +1 more source