Microbial communities in glacial ecosystems are diverse, active, and subjected to strong viral pressures and infection rates. In this study we analyse putative virus genomes assembled from three dsDNA viromes from cryoconite hole ecosystems of Svalbard ...
Christopher Mark Bellas +2 more
doaj +1 more source
Identification of novel protein-protein interactions of Yersinia pestis type III secretion system by yeast two hybrid system. [PDF]
Type III secretion system (T3SS) of the plague bacterium Y. pestis encodes a syringe-like structure consisting of more than 20 proteins, which can inject virulence effectors into host cells to modulate the cellular functions.
Huiying Yang +9 more
doaj +1 more source
Multiple pathways of plasmid DNA transfer in Helicobacter pylori [PDF]
Many Helicobacter pylori (Hp) strains carry cryptic plasmids of different size and gene content, the function of which is not well understood. A subgroup of these plasmids (e.g.
Haas, Rainer +17 more
core +2 more sources
Carbapenemase-producing enterobacteriaceae recovered from a Spanish river ecosystem.
The increasing resistance to carbapenems is an alarming threat in the fight against multiresistant bacteria. The dissemination properties of antimicrobial resistance genes are supported by their detection in a diverse population of bacteria, including ...
Núria Piedra-Carrasco +7 more
doaj +1 more source
Partition of the Linear Plasmid N15: Interactions of N15 Partition Functions with the sop Locus of the F Plasmid [PDF]
ABSTRACT A locus close to one end of the linear N15 prophage closely resembles the sop operon which governs partition of the F plasmid; the promoter region contains similar operator sites, and the two putative gene products have extensive amino acid identity with the SopA and -B proteins of F.
N, Ravin, D, Lane
openaire +2 more sources
ParB Partition Proteins: Complex Formation and Spreading at Bacterial and Plasmid Centromeres
In bacteria, active partition systems contribute to the faithful segregation of both chromosomes and low-copy-number plasmids. Each system depends on a site-specific DNA binding protein to recognize and assemble a partition complex at a centromere-like ...
Barbara Funnell
doaj +1 more source
The ParB-parS partition complexes that bacterial replicons use to ensure their faithful inheritance also find employment in visualization of DNA loci, as less intrusive alternatives to fluorescent repressor-operator systems. The ability of ParB molecules
Sylvain Audibert +6 more
doaj +1 more source
A mutant defective in partitioning of composite plasmid Rms201 [PDF]
Escherichia coli harboring mutant plasmids defective in maintenance stability (from the conjugative plasmid Rms201) showed a wide distribution of ampicillin resistance levels, as well as increased frequency of plasmid loss from the cell. The amounts of covalently closed circular deoxyribonucleic acid of mutant plasmid Rms268 and parental plasmid Rms201
Y, Ike, H, Hashimoto, S, Mitsuhashi
openaire +2 more sources
Examination of plasmid stability in a recombinant organism used in continuous fermentation [PDF]
The gene for the enzyme D-GPA dehalogenase has been cloned into a multi-copy plasmid to produce the vector pTB316. It was transformed into two strains of Pseu- domonas putida , AJl-23 and NCIB11767 in the hope of increasing production of this enzyme. The
O'Neill, Mary P.
core
Golgi enzymes are retrieved from the plasma membrane to the trans‐Golgi network
Golgi enzymes are traditionally considered resident proteins retained within the Golgi apparatus. Here, we demonstrate that a subset transiently reaches the cell surface and is subsequently retrieved to the trans‐Golgi network via retrograde transport. Using a nanobody‐based toolkit, we uncover a dynamic trafficking cycle of several Golgi enzymes.
Dominik P. Buser, Tina Junne
wiley +1 more source

