Construction of biologically functional bacterial plasmids in vitro.
Proceedings of the National Academy of Sciences of the United States of America, 1973 The construction of new plasmid DNA species by in vitro joining of restriction endonuclease-generated fragments of separate plasmids is described. Newly constructed plasmids that are inserted into Escherichia coli by transformation are shown to be ...
S. Cohen +3 more
semanticscholar +1 more source
Within-host bacterial growth dynamics with both mutation and horizontal gene transfer [PDF]
arXiv, 2020 The evolution and emergence of antibiotic resistance is a major public health concern. The understanding of the within-host microbial dynamics combining mutational processes, horizontal gene transfer and resource consumption, is one of the keys to solve this problem.
arxiv
Detection Of DNA Damage By Use Of Escherichia Coli Carrying recA\u27::lux, uvrA\u27::lux, And alkA\u27::lux Reporter Plasmids [PDF]
, 1997 Plasmids were constructed in which DNA damage-inducible promoters recA, uvrA, and alkA from Escherichia coli were fused to the Vibrio fischeri luxCDABE operon. Introduction of these plasmids into E. coli allowed the detection of a dose-dependent response
Belkin, S. +4 more
core +1 more source
Designer diatom episomes delivered by bacterial conjugation. [PDF]
, 2015 Eukaryotic microalgae hold great promise for the bioproduction of fuels and higher value chemicals. However, compared with model genetic organisms such as Escherichia coli and Saccharomyces cerevisiae, characterization of the complex biology and ...
Allen, Andrew E +19 more
core +2 more sources
Electromediated formation of DNA complexes with cell membranes and its consequences for gene delivery [PDF]
arXiv, 2011 Electroporation is a physical method to induce the uptake of therapeutic drugs and DNA, by eukaryotic cells and tissues. The phenomena behind electro-mediated membrane permeabilization to plasmid DNA have been shown to be significantly more complex than those for small molecules.
arxiv
The Plasmid Mobilome of the Model Plant-Symbiont Sinorhizobium meliloti: Coming up with New Questions and Answers [PDF]
, 2014 Rhizobia are Gram-negative Alpha- andBetaproteobacteria living in the underground that have theability to associate with legumes for the establishment ofnitrogen-fixing symbioses.Sinorhizobium melilotiinparticular—the symbiont ofMedicago,Melilotus ...
Lagares, Antonio +2 more
core +2 more sources
The coevolution of toxin and antitoxin genes drives the dynamics of bacterial addiction complexes and intragenomic conflict [PDF]
, 2012 Bacterial genomes commonly contain ‘addiction’ gene complexes that code for both a toxin and a corresponding antitoxin. As long as both genes are expressed, cells carrying the complex can remain healthy.
Brown, Sam P. +3 more
core +1 more source
Positive selection and compensatory adaptation interact to stabilize non-transmissible plasmids
Nature Communications, 2014 Plasmids are important drivers of bacterial evolution, but it is challenging to understand how plasmids persist over the long term because plasmid carriage is costly. Classical models predict that horizontal transfer is necessary for plasmid persistence,
Á. S. Millán +8 more
semanticscholar +1 more source
Transfer of antibiotic resistance in Staphylococcus aureus [PDF]
, 2017 Staphylococcus aureus is a serious human pathogen with remarkable adaptive powers. Antibiotic-resistant clones rapidly emerge mainly by acquisition of antibiotic-resistance genes from other S. aureus strains or even from other genera.
Haaber, Jakob +2 more
core +1 more source
Dominance of the Inducible State in Strains of Staphylococcus aureus Containing Two Distinct Penicillinase Plasmids [PDF]
, 1965 M. H. Richmond
openalex +1 more source