Results 151 to 160 of about 890,612 (362)

Localization of tyrosine kinase-coding region in v-abl oncogene by the expression of v-abl-encoded proteins in bacteria [PDF]

, 1985
A series of plasmids containing different segments of the v-abl oncogene have been constructed to express different portions of the v- abl protein in bacteria.
Baltimore, David, Wang, Jean Y. J.
core  

Homologous expression and purification of human HAX‐1 for structural studies

FEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

Auxotrophy-Independent Plasmid Shuttle Vectors for Applications in Diverse Yeasts

Applied Microbiology
Plasmid shuttle vectors are a common tool used to study yeast physiology. The majority of yeast plasmids have been optimized for Saccharomyces cerevisiae lab strain compatibility, relying on auxotrophic complementation as their selective property.
Jeremy R. Smith, Christine D. Sislak, Pedro Fernandez Mendoza, Laurin Carmichael, Alisha G. Lewis, Anqi Chen, Glycine Z. Jiang, Patrick A. Gibney   +7 more
doaj   +1 more source

Selection of a Multidrug Resistance Plasmid by Sublethal Levels of Antibiotics and Heavy Metals

, 2014
Erik Gullberg, Lisa Albrecht, Christoffer Karlsson, Linus Sandegren, Dan I. Andersson   +4 more
openalex   +1 more source

Sequence-independent, site-specific incorporation of chemical modifications to generate light-activated plasmids

, 2023
Khoa Chung, Michael J. Booth
openalex   +2 more sources

ATG4B is required for mTORC1‐mediated anabolic activity and is associated with clinical outcomes in non‐small cell lung cancer

FEBS Open Bio, EarlyView.
The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan, Bethany C. Guerra, Selina Uranga, Jessica M. Cardin, Steven E. Riechman, Mariana Janini Gomes, James D. Fluckey   +6 more
wiley   +1 more source

Transformation of Gardnerella vaginalis with a Bifidobacterium-Escherichia coli shuttle vector plasmid

Microbiology Spectrum
Gardnerella spp. significantly influence female reproductive health and are indicators of bacterial vaginosis, a common gynecological disorder. Lack of genetic tools for Gardnerella spp.
B. M. D. N. Kularatne, Janet E. Hill
doaj   +1 more source

Lagging-Strand Replication from the ssoA Origin of Plasmid pMV158 in Streptococcus pneumoniae : In Vivo and In Vitro Influences of Mutations in Two Conserved ssoA Regions [PDF]

, 1998
M. Gabriela Kramer, Saleem A. Khan, Manuel Espinosa   +2 more
openalex   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

FEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq, Nestor G. Casillas‐Vega, Alma Gomez‐Loredo, Xristo Zarate   +3 more
wiley   +1 more source

Mechanism underlying synergic activation of Tyrosinase promoter by MITF and IRF4 [PDF]

, 2017
Background: The transcription factor interferon regulatory factor 4 (IRF4) was identified to be involved in human pigmentation by genome-wide association studies (GWASs).
Cai, Xinzhang, Chen, Hongsheng, Feng, Yong, He, Chufeng, Li, Jiada, Liu, Huadie, Liu, Xueming, Liu, Yalan, Mei, Lingyun, Peng, Zhen, Song, Jian, Vleminckx, Kris   +11 more
core   +1 more source