Results 141 to 150 of about 5,639,155 (335)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

open access: yesFEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen   +9 more
wiley   +1 more source

Development and optimization of a high-performance liquid chromatography method for the determination of theaflavin purity

open access: yesZhongguo shipin weisheng zazhi
ObjectivesA high-performance liquid chromatography method was developed to determine the purity of theaflavins, a food additive.MethodsThe samples were dissolved in ethanol-water (2∶8,V/V) solution and separated by C18 reversed-phase chromatography with ...
TIAN Hongyun   +7 more
doaj   +1 more source

Enforcement of platform private regulation and EU consumer law

open access: yes
It is widely acknowledged that platforms hold significant regulatory authori-ty, influencing not only digital governance but also reinterpreting the very foundations of contractual relations.
CANTERO GAMITO, Marta
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Quantitative proteomic analysis reveals different characteristics of bladder cancer cells after exposure to bisphenol A

open access: yesFEBS Open Bio, EarlyView.
Bisphenol A (BPA), a common chemical in plastics, exerts dual effects on bladder cancer cells: low doses promote growth and migration, while high doses suppress growth and migration. Multi‐omics and bioinformatics reveal BPA acts via MAPK and inflammatory pathways.
Shaomin Niu   +10 more
wiley   +1 more source

Establishment and optimization of a HPLC method for determination of L-threonic acid in magnesium L-threonate

open access: yesZhongguo shipin weisheng zazhi
ObjectiveTo develop a high-performance liquid chromatography-based method for the determination of L-threonate in magnesium L-threonate products.MethodsThe samples were dissolved in water and separated by C18 reversed-phase chromatography with 0.1 ...
TIAN Hongyun   +7 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

News media lobbying and the EU’s Digital Services Act: Strategies and experiences from the small non-EU state of Norway

open access: yesNordicom Review
The formation of the Digital Services Act (DSA) in the European Union (EU), aiming to regulate platform power, provided a unique policy window to secure media-specific interests vis-à-vis platforms. This study investigates how Norwegian news media actors
Olsen Gunhild Ring   +3 more
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

open access: yesFEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee   +6 more
wiley   +1 more source

Why human connection is the true metric of research success

open access: yesFEBS Open Bio, EarlyView.
Human‐centred mentorship can be shaped by mentor attributes, actions, intrinsic drive and career ambition. Drawing on reflections across Singapore and France, as well as workshop insights from FEBS‐IUBMB ENABLE 2024, this article shows that human‐centred mentorship creates the conditions for sustainable growth, well‐being and retention in research ...
Timothy Lin Yun Tan   +3 more
wiley   +1 more source

Shaping the future of European rail : regulation, digitalisation, and fair competition

open access: yes
Published online: January 2026The European rail sector is experiencing a rapid transformation thanks to digitalisation. At the same time, policymakers and industry stakeholders navigate the challenges of regulation, digitalisation, and fair competition ...

core  

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