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Analysis of Methanotroph Community Structure Using a pmoA-Based Microarray

2014
The analysis of methanotroph community composition is relevant to studies of methane oxidation in a number of environments where methane is a significant carbon source. The development and application of a microarray targeting the particulate methane monooxygenase gene (pmoA) have allowed a high-throughput, semiquantitative analysis of the major ...
Guy C J, Abell   +3 more
openaire   +2 more sources

A novel pmoA lineage represented by the acidophilic methanotrophic bacterium Methylocapsa acidophila B2

Archives of Microbiology, 2001
A fragment of the functional gene pmoA, which encodes the active-site polypeptide of particulate methane monooxygenase (pMMO), was PCR-amplified from DNA of the recently described acidophilic methanotrophic bacterium Methylocapsa acidophila B2. This methanotroph was isolated from an acidic Sphagnum peat bog and possesses a novel type III arrangement of
Dedysh, S.   +3 more
openaire   +2 more sources

ARChip Epoxy and ARChip UV for covalent on-chip immobilization of pmoA gene-specific oligonucleotides

Analytical Biochemistry, 2004
ARChip Epoxy and ARChip UV are presented as novel chip platforms for oligonucleotide immobilization. ARChip Epoxy is made of reactive epoxy resin available commercially. ARChip UV consists of photoactivatable poly(styrene-co-4-vinylbenzylthiocyanate).
Claudia, Preininger   +4 more
openaire   +2 more sources

Ultrasonic-assisted organic–inorganic multilayer thin film synthesis and enhanced visible-light phototropy based on PVP /PMoA

Journal of Materials Science, 2021
Novel organic–inorganic layer-by-layer (LbL) multilayers for visible-light phototropy, comprised of polyvinylpyrrolidone (PVP) and phosphomolybdic acid (PMoA), were fabricated via layer-by-layer (LbL) assembly with ultrasonic pretreatment. The characterizations revealed that the Keggin spatial geometry of the PMoA was still maintained in the final ...
Yu-Ting Huang   +3 more
openaire   +1 more source

Instantaneous Visible-light Photochromic Performance of Composite Powders Based on PMoA and ZnO Nanotubes

Chemistry Letters, 2019
Novel visible-light photochromic composites were synthesized by introducing PMoA into ZnO-nanotubes fabricated through a hydrothermal synthesis method.
Tong-tong Yue   +4 more
openaire   +1 more source

The evolution of the AMOA, PMOA and BMOA genes measured by ka/ks ratios.

Proceedings of the West Virginia Academy of Science, 2016
The copper-containing membrane-bound monooxygenase enzyme family consists of enzymes facilitating the oxidation of methane (catalyzed by particulate methane monooxygenase, pMMO) and ammonia (catalyzed by ammonia monooxygenase, AMO), in both aerobic and anaerobic methanotrophs and nitrifiers, respectively, and the oxidation of C2-10 alkanes (catalyzed ...
Evan Lau, Daniel Lukich, Tuan Le
openaire   +1 more source

Spatial heterogeneity of methanotrophs: a geostatistical analysis of pmoA ‐based T‐RFLP patterns in a paddy soil

Environmental Microbiology Reports, 2009
Summary Despite numerous studies on methanotrophs, virtually nothing is known about their spatial heterogeneity in nature. These patterns, however, have strong influences on the interpretations made from analysing microbial processes and community structure. Here we report the first use of geostatistics to analyse
Krause, S., Lüke, C., Frenzel, P.
openaire   +3 more sources

Quantitative PCR of pmoA using a novel reverse primer correlates with potential methane oxidation in Finnish fen

Research in Microbiology, 2009
We report a new reverse primer (A621r) for use with A189f in PCR amplification of pmoA alleles in type II methanotrophs. The new primer combination was used to successfully amplify pmoA in peat monolith samples of various depths taken from fen-type peatlands in Finland.
Tuomivirta, T., Yrjälä, K., Fritze, H.
openaire   +2 more sources

A New Primer to Amplify pmoA Gene From NC10 Bacteria in the Sediments of Dongchang Lake and Dongping Lake

Current Microbiology, 2017
Nitrite-dependent anaerobic methane oxidation (n-damo) is catalyzed by the NC10 phylum bacterium "Candidatus Methylomirabilis oxyfera" (M. oxyfera). Generally, the pmoA gene is applied as a functional marker to test and identify NC10-like bacteria. However, it is difficult to detect the NC10 bacteria from sediments of freshwater lake (Dongchang Lake ...
Shenghui, Wang   +4 more
openaire   +2 more sources

Selective stimulation in a natural community of methane oxidizing bacteria: Effects of copper on pmoA transcription and activity

Soil Biology and Biochemistry, 2013
Abstract Copper is an essential element, but often not readily available to microorganisms potentially causing copper limitation in situ . In most methane oxidizing bacteria (methanotrophs), the copper-containing membrane bound methane monooxygenase (pMMO) catalyzes the first step in methane oxidation.
Ho, A., Luke, C., Reim, A., Frenzel, P.
openaire   +2 more sources

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