Comparison of communities of both methane-producing and metabolizing archaea and bacteria in sediments between the northern South China Sea and coastal Mai Po Nature Reserve revealed by PCR amplification of mcrA and pmoA genes [PDF]
Communities of methanogens, anaerobic methanotrophic (ANME) archaea and aerobic methantrophic bacteria were compared by profiling mcrA and pmoA genes encoded by methyl-coenzyme M reductase alpha subunit and particulate methane monooxygenase alpha subunit,
Zhichao eZhou +3 more
doaj +20 more sources
The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs. [PDF]
The particulate methane monooxygenase gene pmoA, encoding the 27 kDa polypeptide of the membrane-bound particulate methane monooxygenase, was amplified by PCR from DNA isolated from a blanket peat bog and from enrichment cultures established, from the same environment, using methane as sole carbon and energy source.
I R McDonald, J. Murrell
semanticscholar +6 more sources
Evaluation and update of cutoff values for methanotrophic pmoA gene sequences [PDF]
The functional pmoA gene is frequently used to probe the diversity and phylogeny of methane-oxidizing bacteria (MOB) in various environments. Here, we compared the similarities between the pmoA gene and the corresponding 16S rRNA gene sequences of 77 described species covering gamma- and alphaproteobacterial methanotrophs (type I and type II MOB ...
Xi Wen, Sizhong Yang, S. Liebner
semanticscholar +7 more sources
Isolation of a Methylocystis strain containing a novel pmoA-like gene. [PDF]
Abstract A type II methanotrophic bacterium (Methylocystis strain SC2) was isolated from a polluted aquifer and identified based on morphology and on 16S rRNA gene phylogeny. Primers targeting the particulate methane monooxygenase subunit A gene (pmoA) were used to obtain a PCR product from DNA extract of strain SC2.
P. Dunfield +5 more
semanticscholar +7 more sources
Wide Distribution of a Novel pmoA-Like Gene Copy among Type II Methanotrophs, and Its Expression in Methylocystis Strain SC2 [PDF]
ABSTRACT Experiments were conducted to determine if a novel pmoA -like gene ( pmoA2 ) recently discovered in the methane-oxidizing bacterium Methylocystis strain SC2 (P. F. Dunfield, M. Tchawa Yimga, S. D. Dedysh, U. Berger, W. Liesack, and J.
Merlin Tchawa Yimga +4 more
semanticscholar +6 more sources
In natural ecosystems such as forests topographical gradients, species composition variability and seasonality, are the potential drivers of methane (CH 4 ) metabolism, and thus, of the population size and activities of methane oxidizing bacteria (MOB ...
Yashpal Bhardwaj, S. K. Dubey
semanticscholar +2 more sources
Macroecology of methane-oxidizing bacteria: the β-diversity of pmoA genotypes in tropical and subtropical rice paddies [PDF]
Studies addressing microbial biogeography have increased during the past decade, but research on microbial distribution patterns is still in its infancies, and many aspects are only poorly understood. Here, we compared the methanotroph community in paddy
Fiantis, Dian +7 more
core +3 more sources
Maintaining rice yield, soil function, and fertility are essential components of long-term compost fertilization. However, paddy fields are major sources of anthropogenic methane emissions. The aim of the study is to evaluate the changes in soil chemical
Chungwoo Kim +8 more
doaj +2 more sources
Activity and composition of methanotrophic bacterial communities in planted rice soil studied by flux measurements, analyses of pmoA gene and stable isotope probing of phospholipid fatty acids. [PDF]
Summary Methanotrophs in the rhizosphere of rice field ecosystems attenuate the emissions of CH 4 into the atmosphere and thus play an important role for the global cycle of this greenhouse gas.
Minita Shrestha +4 more
semanticscholar +5 more sources
Novel Methanotrophs of the Family Methylococcaceae from Different Geographical Regions and Habitats [PDF]
Terrestrial methane seeps and rice paddy fields are important ecosystems in the methane cycle. Methanotrophic bacteria in these ecosystems play a key role in reducing methane emission into the atmosphere.
Tajul Islam +7 more
doaj +5 more sources

