Comparison of communities of both methane-producing and metabolizing archaea and bacteria in sediments between the northern South China Sea and coastal Mai Po Nature Reserve revealed by PCR amplification of mcrA and pmoA genes [PDF]
Communities of methanogens, anaerobic methanotrophic (ANME) archaea and aerobic methantrophic bacteria were compared by profiling mcrA and pmoA genes encoded by methyl-coenzyme M reductase alpha subunit and particulate methane monooxygenase alpha subunit,
Zhichao eZhou +3 more
doaj +9 more sources
Diversity and habitat preferences of cultivated and uncultivated aerobic methanotrophic bacteria evaluated based on pmoA as molecular marker [PDF]
Methane-oxidizing bacteria are characterized by their capability to grow on methane as sole source of carbon and energy. Cultivation-dependent and –independent methods have revealed that this functional guild of bacteria comprises a substantial diversity
Claudia eKnief
doaj +2 more sources
In the present work, both 16S rRNA and pmoA gene-based PCR primers were employed successfully to study the diversity and distribution of n-damo bacteria in the surface and lower layer sediments at the coastal Mai Po wetland.
Zhou, Zhichao, Gu, Ji-Dong, Chen, Jing
core +5 more sources
The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs [PDF]
The particulate methane monooxygenase gene pmoA, encoding the 27 kDa polypeptide of the membrane-bound particulate methane monooxygenase, was amplified by PCR from DNA isolated from a blanket peat bog and from enrichment cultures established, from the same environment, using methane as sole carbon and energy source.
I R, McDonald, J C, Murrell
openaire +2 more sources
Detection of methanotrophs with highly divergent pmoA genes from Arctic soils [PDF]
Tundra soil samples from the Canadian Arctic community, Kuujjuaq, were analyzed for the presence of the soluble (sMMO) and particulate (pMMO) methane monooxygenase genes. Total genomic DNA extracted from these soils was used as template for PCR using sMMO- and pMMO-specific primers, mmoX1-mmoX2 and A189-A682, respectively.
Pacheco-Oliver, Maria +4 more
openaire +2 more sources
Enrichment of denitrifying methane-oxidizing microorganisms using up-flow continuous reactors and batch cultures. [PDF]
Denitrifying anaerobic methane oxidizing (DAMO) microorganisms were enriched from paddy field soils using continuous-flow and batch cultures fed with nitrate or nitrite as a sole electron acceptor. After several months of cultivation, the continuous-flow
Masashi Hatamoto +7 more
doaj +1 more source
Root-associated aerobic methanotrophs play an important role in regulating methane emissions from the wetlands. However, the influences of the plant genotype on root-associated methanotrophic structures, especially on active flora, remain poorly ...
Jing Cui +6 more
doaj +1 more source
Maintaining rice yield, soil function, and fertility are essential components of long-term compost fertilization. However, paddy fields are major sources of anthropogenic methane emissions. The aim of the study is to evaluate the changes in soil chemical
Chungwoo Kim +8 more
doaj +1 more source
Methane-cycling communities in a permafrost-affected soil on Herschel Island, Western Canadian Arctic: active layer profiling ofmcrAandpmoAgenes [PDF]
In Arctic wet tundra, microbial controls on organic matter decomposition are likely to be altered as a result of climatic disruption. Here, we present a study on the activity, diversity and vertical distribution of methane-cycling microbial communities in the active layer of wet polygonal tundra on Herschel Island.
Barbier, B. +6 more
openaire +4 more sources
Vertical distribution of methane oxidation and methanotrophic response to elevated methane concentrations in stratified waters of the Arctic fjord Storfjorden (Svalbard, Norway) [PDF]
The bacterially mediated aerobic methane oxidation (MOx) is a key mechanism in controlling methane (CH₄) emissions from the world’s oceans to the atmosphere.
E. Helmke +13 more
core +2 more sources

