Anaerobic methane-oxidizing bacterial communities in sediments of a drinking reservoir, Beijing, China [PDF]
Annals of Microbiology, 2020 Purpose Nitrate/nitrite-dependent anaerobic methane oxidation (N-DAMO) connects the global cycle of carbon and nitrogen in aquatic ecosystems. The aims of this study were to investigate the spatiotemporal variation of N-DAMO bacteria and its driving ...
Yang Liu +5 more
doaj +21 more sources
Classification of pmoA amplicon pyrosequences using BLAST and the lowest common ancestor method in MEGAN [PDF]
Frontiers in Microbiology, 2014 The classification of high-throughput sequencing data of protein-encoding genes is not as well established as for 16S rRNA. The objective of this work was to develop a simple and accurate method of classifying large datasets of pmoA sequences, a common ...
Marc Gregory Dumont +5 more
doaj +6 more sources
Macroecology of methane-oxidizing bacteria: the β-diversity of pmoA genotypes in tropical and subtropical rice paddies [PDF]
Environmental Microbiology, 2013 Studies addressing microbial biogeography have increased during the past decade, but research on microbial distribution patterns is still in its infancies, and many aspects are only poorly understood. Here, we compared the methanotroph community in paddy
Fiantis, Dian +7 more
core +8 more sources
SurfacesThe recyclable paper based on photochromic materials not only reduces the pollution in the paper manufacture process, but also reduces the pollution caused by the use of ink, which receives wide attention.
Wanqing Zhao +3 more
doaj +2 more sources
Diversity and habitat preferences of cultivated and uncultivated aerobic methanotrophic bacteria evaluated based on pmoA as molecular marker [PDF]
Frontiers in Microbiology, 2015 Methane-oxidizing bacteria are characterized by their capability to grow on methane as sole source of carbon and energy. Cultivation-dependent and –independent methods have revealed that this functional guild of bacteria comprises a substantial diversity
Claudia eKnief
doaj +3 more sources
Isolation of a Methylocystis strain containing a novel pmoA-like gene [PDF]
FEMS Microbiology Ecology, 2002 Abstract A type II methanotrophic bacterium (Methylocystis strain SC2) was isolated from a polluted aquifer and identified based on morphology and on 16S rRNA gene phylogeny. Primers targeting the particulate methane monooxygenase subunit A gene (pmoA) were used to obtain a PCR product from DNA extract of strain SC2.
Dunfield, P. F. +5 more
openaire +4 more sources
Evaluation and update of cutoff values for methanotrophic pmoA gene sequences [PDF]
Archives of Microbiology, 2016 The functional pmoA gene is frequently used to probe the diversity and phylogeny of methane-oxidizing bacteria (MOB) in various environments. Here, we compared the similarities between the pmoA gene and the corresponding 16S rRNA gene sequences of 77 described species covering gamma- and alphaproteobacterial methanotrophs (type I and type II MOB ...
Wen, X., Yang, S., Liebner, S.
openaire +4 more sources
Detection of methanotrophs with highly divergent pmoA genes from Arctic soils [PDF]
FEMS Microbiology Letters, 2002 Tundra soil samples from the Canadian Arctic community, Kuujjuaq, were analyzed for the presence of the soluble (sMMO) and particulate (pMMO) methane monooxygenase genes. Total genomic DNA extracted from these soils was used as template for PCR using sMMO- and pMMO-specific primers, mmoX1-mmoX2 and A189-A682, respectively.
Pacheco-Oliver, Maria +4 more
openaire +2 more sources
The particulate methane monooxygenase gene pmoA and its use as a functional gene probe for methanotrophs [PDF]
FEMS Microbiology Letters, 2006 The particulate methane monooxygenase gene pmoA, encoding the 27 kDa polypeptide of the membrane-bound particulate methane monooxygenase, was amplified by PCR from DNA isolated from a blanket peat bog and from enrichment cultures established, from the same environment, using methane as sole carbon and energy source.
I R, McDonald, J C, Murrell
openaire +3 more sources
Spatial Heterogeneity in Methane Biogeochemistry and Prokaryotic Community Structure in Sub-Arctic Waterbodies in Northern Canada. [PDF]
Environ MicrobiolProkaryotic communities and methane dynamics differed markedly across 16 sub‐Arctic waterbodies. Maximum depth and permafrost thaw emerged as key drivers, with non‐stratified thaw‐affected sites hosting distinct communities and elevated methane fluxes, highlighting their potential role as emerging hotspots of methane emissions.
Szylit A +8 more
europepmc +2 more sources