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Analysis of methanotroph community composition using a pmoA-based microbial diagnostic microarray

Nature Protocols, 2011
Microbial diagnostic microarrays (MDMs) are highly parallel hybridization platforms containing multiple sets of immobilized oligonucleotide probes used for parallel detection and identification of many different microorganisms in environmental and clinical samples. Each probe is approximately specific to a given group of organisms. Here we describe the
Nancy, Stralis-Pavese   +3 more
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Analysis of Methanotroph Community Structure Using a pmoA-Based Microarray

2014
The analysis of methanotroph community composition is relevant to studies of methane oxidation in a number of environments where methane is a significant carbon source. The development and application of a microarray targeting the particulate methane monooxygenase gene (pmoA) have allowed a high-throughput, semiquantitative analysis of the major ...
Guy C J, Abell   +3 more
openaire   +2 more sources

A novel pmoA lineage represented by the acidophilic methanotrophic bacterium Methylocapsa acidophila B2

Archives of Microbiology, 2001
A fragment of the functional gene pmoA, which encodes the active-site polypeptide of particulate methane monooxygenase (pMMO), was PCR-amplified from DNA of the recently described acidophilic methanotrophic bacterium Methylocapsa acidophila B2. This methanotroph was isolated from an acidic Sphagnum peat bog and possesses a novel type III arrangement of
Dedysh, S.   +3 more
openaire   +2 more sources

ARChip Epoxy and ARChip UV for covalent on-chip immobilization of pmoA gene-specific oligonucleotides

Analytical Biochemistry, 2004
ARChip Epoxy and ARChip UV are presented as novel chip platforms for oligonucleotide immobilization. ARChip Epoxy is made of reactive epoxy resin available commercially. ARChip UV consists of photoactivatable poly(styrene-co-4-vinylbenzylthiocyanate).
Claudia, Preininger   +4 more
openaire   +2 more sources

Ultrasonic-assisted organic–inorganic multilayer thin film synthesis and enhanced visible-light phototropy based on PVP /PMoA

Journal of Materials Science, 2021
Novel organic–inorganic layer-by-layer (LbL) multilayers for visible-light phototropy, comprised of polyvinylpyrrolidone (PVP) and phosphomolybdic acid (PMoA), were fabricated via layer-by-layer (LbL) assembly with ultrasonic pretreatment. The characterizations revealed that the Keggin spatial geometry of the PMoA was still maintained in the final ...
Yu-Ting Huang   +3 more
openaire   +1 more source

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