Results 1 to 10 of about 331 (141)

Whole genome characterization and diagnostics of prunus necrotic ringspot virus (PNRSV) infecting apricot in India [PDF]

open access: yesScientific Reports, 2023
Prunus necrotic ringspot virus (PNRSV) is a pathogen that infects Prunus species worldwide, causing major economic losses. Using one and two-step RT-PCR and multiplex RT-PCR, the whole genome of the PNRSV-infecting apricot was obtained and described in ...
Md Salik Noorani   +2 more
exaly   +5 more sources

Analysis of intra-host genetic diversity of Prunus necrotic ringspot virus (PNRSV) using amplicon next generation sequencing

open access: yesPLoS ONE, 2017
PCR amplicon next generation sequencing (NGS) analysis offers a broadly applicable and targeted approach to detect populations of both high- or low-frequency virus variants in one or more plant samples. In this study, amplicon NGS was used to explore the
Wycliff M Kinoti   +2 more
exaly   +5 more sources

Selection and Validation of Reference Genes for Gene Expression Studies Using Quantitative Real-Time PCR in Prunus Necrotic Ringspot Virus-Infected Cucumis sativus [PDF]

open access: yesViruses, 2022
Several members of the genus Ilarvirus infect fruit trees and are distributed worldwide. Prunus necrotic ringspot virus (PNRSV) is one of the most prevalent viruses, causing significant losses.
Zhenfei Dong, Binhui Zhan, Shifang Li
doaj   +2 more sources

Characterization of Prunus Necrotic Ringspot Virus and Cherry Virus A Infecting Myrobalan Rootstock [PDF]

open access: yesViruses, 2023
Prunus necrotic ringspot virus (PNRSV) and cherry virus A (CVA) are two viruses that mainly infect plants of the genus Prunus. Full-length sequences of these two viruses, collected in the Czech Republic from Prunus cerasifera plants, were obtained via ...
Karima Ben Mansour   +3 more
doaj   +2 more sources

Fluctuations of Prunus Necrotic Ringspot Virus (PNRSV) at Various Phenological Stages in Peach Cultivars [PDF]

open access: yesPlant Disease, 1999
Fluctuations in Prunus necrotic ringspot virus (PNRSV) concentration were researched in single plants of six peach (Prunus persicae) cultivars—Kurakata, Red Haven, Nectar Red, Start Delicious, Meadowlark, and Loadel—by double antibody sandwich-enzyme-linked immunosorbent assay (DAS-ELISA) of dormant buds (May, June), flowers (September), new sprouts ...
A Dal, Zotto   +3 more
exaly   +3 more sources

Investigating the Occurrence of Viruses in Sweet Cherry in China and Developing Multiplex RT-PCR Assays for Their Detection [PDF]

open access: yesPlants
Sweet cherry (Prunus avium L.) cultivation in China covers an estimated area of 25,600 hectares, representing more than one-third of the global total. Viral diseases present a serious challenge to cherry production worldwide; however, the phytosanitary ...
Jinying Wang   +8 more
doaj   +2 more sources

Prunus Yapraklarında Prunus Necrotic Ring Spot PNRSV ve Apple Chlorotic Leaf Spot ACLSV Virüslerinin Dağılımı

open access: yesJournal of Agricultural Sciences, 2005
Prunus necrotic ringspot virus PNRSV ’ü ile infekteli Prunus mahaleb ve Apple chlorotic leafspot virus ACLSV ’ü ile infekteli şeftali P. persica L. yapraklarının farklı bölgelerinden alınan doku diskleri bu virüslerin yaprak dokusundaki dağılımlarını
Mustafa Usta   +4 more
doaj   +4 more sources

Studies on Parameters Influencing the Performance of Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) in Detecting Prunus Necrotic Ringpot Virus (PNRSV)

open access: yesPhytopathologia Mediterranea, 2005
In order to have a more detailed understanding of the various factors influencing a reverse transcriptase polymerase chain reaction (RT-PCR), a number of important parameters such as Mg+2, primer, enzyme concentration and others were optimized for the ...
M. Usta, H.M. Sipahioglu, B. Polat
doaj   +3 more sources

Plant virus diversity in bee and pollen samples from apple (Malus domestica) and sweet cherry (Prunus avium) agroecosystems [PDF]

open access: yesFrontiers in Plant Science
IntroductionHoney bee (Apis mellifera) pollination is widely used in tree fruit production systems to improve fruit set and yield. Many plant viruses can be associated with pollen or transmitted through pollination, and can be detected through bee ...
Malek Smadi   +11 more
doaj   +2 more sources

Efficiency of a chemo-thermotherapy technique for eliminating Arabis mosaic virus (ArMV) and Prunus necrotic ringspot virus (PNRSV) from in vitro rose plantlets

open access: yesJournal of Crop Protection, 2016
Mosaic is presumably the most commonly encountered viral disease in roses. We have developed chemo-thermotherapy for eliminating Arabis mosaic virus (ArMV) and Prunus necrotic ringspot virus (PNRSV) from rose plants.
Amir Modarresi Chahardehi   +3 more
doaj   +2 more sources

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