Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
An Efficient Operator for the Change Point Estimation in Partial Spline Model. [PDF]
Commun Stat Simul Comput, 2015 Han SW, Zhong H, Putt M.
europepmc +1 more source
Study on improvement of frost point estimation of residual water in high pressure hydrogen.
, 2017 Kenji Ishida +5 more
openalex +1 more source
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
FEBS Open Bio, EarlyView.Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
An auxiliary gaze point estimation method based on facial normal. [PDF]
Pattern Anal Appl, 2016 Sun W, Sun N, Guo B, Jia W, Sun M.
europepmc +1 more source
Erratum: ‘Commutation point estimation for sensorless brushless DC motor using back‐electromagnetic force change rate by least‐square method’ [PDF]
, 2015 Joonhong Kim, Sun-Kyu Kim, Jung-Hwan Lim
openalex +1 more source
FEBS Open Bio, EarlyView.Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane +11 more
wiley +1 more source
Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis
FEBS Open Bio, EarlyView.Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho +4 more
wiley +1 more source
Basketball Fixed-point Shot Hit Prediction Based on Human Pose Estimation Algorithm
Xi Li, Jiao Hua
openalex +2 more sources