Results 111 to 120 of about 85,251 (307)
FEBS Open Bio, EarlyView.CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Farm Heterogeneity and Efficiency in Polish Agriculture: A Stochastic Frontier Analysis
This paper deals with the estimation of a random coefficient model. The virtue of this approach is that it considers firm heterogeneity, which conventional SFA models do not.
Hockmann, Heinrich, Pieniadz, Agata
core +2 more sources
FEBS Open Bio, EarlyView.Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley +1 more source
FEBS Open Bio, EarlyView.Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn +4 more
wiley +1 more source
The Bulgarian-Polish-Russian parallel corpus
, 2015 The Bulgarian-Polish-Russian parallel corpus The Semantics Laboratory Team of Institute of Slavic Studies of Polish Academy of Sciences is planning to begin work on the creation of a Bulgarian-Polish-Russian parallel corpus. The three selected languages
Maksim Duškin +3 more
core +1 more source
FEBS Open Bio, EarlyView.Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei +3 more
wiley +1 more source
FEBS Open Bio, EarlyView.Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Australia in Polish Literature
, 2011 Australia has never became a popular topic in Polish literature. However, it was present in Polish writings since the Age of Enlightenment. The aim of the text is to analyse different ways in which Australian motifs were used in different literary ...
Bąk, Magdalena
core
FEBS Open Bio, EarlyView.This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
Perspektywy Kultury, 2019 Albania is a small country in Europe, which was under Turkish occupation for nearly five centuries. It did not regain its independence until 28 November 1912. During the occupation there was almost no foreign literature translated into Albanian, as more than 85% of the population were illiterate and in general there were no scientific institutions or ...
openaire +3 more sources