The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis [PDF]
Biochemical Journal, 1967 1. Gels were prepared with recrystallized acrylamide and bisacrylamide. Electrophoresis was in tris-sodium acetate-EDTA buffer for 0.5 to 3hr. Gels were scanned at 280 or 265mmu. Techniques are described for slicing and radioactive counting. 2.
UE Loening
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PEPPI-MS: Polyacrylamide-Gel-Based Prefractionation for Analysis of Intact Proteoforms and Protein Complexes by Mass Spectrometry. [PDF]
J Proteome Res, 2020 Prefractionation of complex mixtures of proteins derived from biological samples is indispensable for proteome analysis via top-down mass spectrometry (MS). Polyacrylamide gel electrophoresis (PAGE), which enables high-resolution protein separation based
Takemori A +14 more
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Polyacrylamide Gel Electrophoresis.
Cold Spring Harbor Protocols, 2020 Cross-linked chains of polyacrylamide can be used as electrically neutral gels to separate double-stranded DNA fragments according to size and single-stranded DNAs according to size and conformation.
Michael R Green, J. Sambrook
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Encapsulation of pectinase within polyacrylamide gel: characterization of its catalytic properties for continuous industrial uses [PDF]
Heliyon, 2020 Pectinase as a biocatalyst play a significant role in food and textile industries. In this study, the pectinase was immobilized by encapsulation within polyacrylamide gel to enhance its catalytic properties and ensure the reusability for continuous ...
Haneef Ur Rehman +6 more
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Basics and recent advances of two dimensional- polyacrylamide gel electrophoresis. [PDF]
Clin Proteomics, 2014 Gel- based proteomics is one of the most versatile methods for fractionating protein complexes. Among these methods, two dimensional- polyacrylamide gel electrophoresis (2-DE) represents a mainstay orthogonal approach, which is popularly used to ...
Magdeldin S +8 more
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Nanoparticle Dynamics near Polyacrylamide Gel Interfaces [PDF]
ACS Polymers AuBrittany K. Roopnarine +4 more
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Analysis of Mitochondrial Respiratory Chain Supercomplexes Using Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE). [PDF]
Curr Protoc Mouse Biol, 2016 Jha P, Wang X, Auwerx J.
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A novel mechanism of "metal gel-shift" by histidine-rich Ni2+-binding Hpn protein from Helicobacter pylori strain SS1. [PDF]
PLoS ONE, 2017 Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is a universally used method for determining approximate molecular weight (MW) in protein research.
Rahul Mahadev Shelake +4 more
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Bioscience Journal, 2023 1,4-alpha-D-glucan glucanohydrolase is among the most widely used commercial hydrolytic enzymes acting randomly on the glycosidic linkages of starch resulting in its saccharification and liquefaction.
Aliya Riaz +5 more
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Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.
Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022 Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (
Ammar Q. Muhammed +2 more
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