Results 11 to 20 of about 446,459 (290)
Denaturing Polyacrylamide Gel Electrophoresis [PDF]
Current Protocols in Nucleic Acid Chemistry, 1994 AbstractThin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single‐stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. Such gels are uniquely suited for nucleic acid sequence analysis, which is required, for instance, for all ...
Albright, Lisa M., Slatko, Barton E.
openaire +4 more sources
Efficacy of gel electrophoresis for proteins and biotechnological products –an overview.
Al-Mustansiriyah Journal of Pharmaceutical Sciences, 2022 Nucleic acids or proteins electrophoresed within a matrix or gel that immersed in a buffer provides ions needed to carry a current and for pH maintenance at a relatively constant value. Sodium dodecyl sulphate–polyacrylamide gel electrophoresis (
Ammar Q. Muhammed +2 more
doaj +1 more source
Proteomic analysis of mucopolysaccharidosis I mouse brain with two-dimensional polyacrylamide gel electrophoresis. [PDF]
Mol Genet Metab, 2017 Ou L, Przybilla MJ, Whitley CB.
europepmc +3 more sources
Electroblotting from Polyacrylamide Gels [PDF]
Current Protocols in Protein Science, 2015 AbstractTransferring proteins from polyacrylamide gels onto retentive membranes is now primarily used for immunoblotting. A second application that was quite common up to about a decade ago was electroblotting of proteins for N‐terminal and internal sequencing using Edman chemistry.
Aaron, Goldman +3 more
openaire +2 more sources
Separation of apolipoprotein B species by agarose-acrylamide gel electrophoresis.
Journal of Lipid Research, 1988 Human apolipoprotein (apo) B has been recognized to exist in two different forms designated apoB-100 and apoB-48. The two apoB forms are usually separated by NaDodSO4 gel electrophoresis with a low percentage polyacrylamide gel in a tube gel apparatus ...
C Gabelli +3 more
doaj +1 more source
A simple drying solution that minimizes cracking during air-drying of polyacrylamide gels
BioTechniques, 2023 Sodium dodecyl sulfate–polyacrylamide gel electrophoresis is a routine technique used in biochemistry. Air-drying is an economical method of gel preservation that does not require expensive equipment.
Kristan M Gomez +5 more
doaj +1 more source
SDS Agarose Gels for Analysis of Proteins
BioTechniques, 1998 A new agarose-based protein electrophoresis gel system is described. The system consists of a highly resolving agarose, MetaPhor® XR (FMC BioProducts, Rockland, ME, USA) dissolved in urea and TBE buffer and a stacking gel composed of a high gel strength ...
Minjie Wu, Noriko Kusukawa
doaj +1 more source
Carbohydrate Affinity PAGE for the Study of Carbohydrate-Binding Proteins
BioTechniques, 1998 Immobilized neoglycoconjugates covalently cross-linked into a polyacrylamide gel can be used to detect and characterize carbohydrate-binding proteins.
Phil D. Rye, Nicolai V. Bovin
doaj +1 more source
Facile synthesis of high-entropy zirconate nanopowders and their sintering behaviors
Journal of Advanced Ceramics, 2023 The challenge in synthesizing high-entropy ceramic (HEC) nanopowders is to suppress severe grain coarsening and particle agglomeration, which occur at elevated temperatures. This challenge could be addressed by the polyacrylamide gel method. In this work,
Lin Tan +6 more
doaj +1 more source
Journal of Pharmacopuncture, 2006 Objectives : This study was conducted to carry out Purification of Melittin and other peptide components from Bee Venom using gel filtration chromatography and propionic acid/urea polyacrylamide gel electrophoresis Methods : Melittin and other peptide ...
Young Chon Choi +2 more
doaj +1 more source