Results 11 to 20 of about 431,758 (295)

Denaturing Polyacrylamide Gel Electrophoresis [PDF]

open access: yesCurrent Protocols in Nucleic Acid Chemistry, 1994
AbstractThin polyacrylamide gels that contain a high concentration of urea as a denaturant are capable of resolving short (<500 nucleotides) single‐stranded fragments of DNA or RNA that differ in length by as little as one nucleotide. Such gels are uniquely suited for nucleic acid sequence analysis, which is required, for instance, for all ...
Albright, Lisa M., Slatko, Barton E.
openaire   +4 more sources

Swelling of polyacrylamide gels in polyacrylamide solutions

open access: yesJournal of Polymer Science Part B: Polymer Physics, 1998
Swelling behavior of polyacrylamide (PAAm) and polyacrylamide-co-poly- acrylic acid (PAAm-co-PAAc) gels was investigated in aqueous solutions of monodis- perse PAAms with molecular weights (M V w) ranging from 1.5 1 10 3 to 5 1 10 6 g/mol. The volume of the gels decreases as the PAAm concentration in the external solution increases.
Oguz Okay   +4 more
openaire   +3 more sources

Acute-phase protein concentration in bronchoalveolar lavage fluid from healthy horses [PDF]

open access: yesPesquisa Veterinária Brasileira, 2021
: Bronchoalveolar lavage fluid (BALF) was analyzed to obtain information on leakage of acute-phase proteins from the blood into the respiratory lumen and about local synthesis.
Paula Alessandra Di Filippo   +4 more
doaj   +1 more source

Formation and structure of polyacrylamide gels

open access: yesJournal of Applied Polymer Science, 1996
SYNOPSIS Acrylamide and N,N-methylenebis(acry1amide) (AAm-Bis) copolymerization has been investigated in water at a monomer concentration of 1.8 w/v %. Conversion of monomer and pendant vinyl groups was measured as a function of the reaction time up to the onset of macrogelation.
Oguz Okay   +2 more
openaire   +4 more sources

Purification and characterization of acid phosphatase from a germinating black gram (Vigna mungo L.) seedling [PDF]

open access: yesArchives of Biological Sciences, 2011
An acid phosphatase has been isolated and purified from an extract of a germinating black gram seedling. The method was accomplished by gel filtration of a germinating black gram seedling crude extract on sephadex G-75 followed by ion exchange ...
Asaduzzaman A.K.M.   +2 more
doaj   +1 more source

The guideline for western blotting assay

open access: yesFood Frontiers, 2022
Western blotting is a technique for detecting protein developed based on protein electrophoresis separation and antigen‐antibody detection. It combines the high resolution of SDS polyacrylamide gel electrophoresis with the specificity of antigen‐antibody
Fang Wang   +5 more
doaj   +1 more source

Experimental Study to Investigate the Effect of Polyacrylamide Gel to Reduce the Lost Circulation

open access: yesIraqi Journal of Chemical and Petroleum Engineering, 2023
One of the challenging issues encountered during drilling operations is the lost circulation. Numerous issues might arise because of losses, such as wasting of time and higher drilling cost.
Ali K. Al-Delfi   +3 more
doaj   +1 more source

Polyacrylamide Polymer Gel Systems for Conformance Control Technology: A Review

open access: yesIraqi Journal of Chemical and Petroleum Engineering, 2022
Low oil extraction and early high water production are caused in part by reservoir heterogeneity. Huge quantities of water production are prevalent issues that happen in older reservoirs.
Ali Al-delfi, Faleh H. M. Al-Mahdawi
doaj   +1 more source

B cell mechanobiology in health and disease: emerging techniques and insights into therapeutic responses

open access: yesFEBS Letters, EarlyView.
B cells sense external mechanical forces and convert them into biochemical signals through mechanotransduction. Understanding how malignant B cells respond to physical stimuli represents a groundbreaking area of research. This review examines the key mechano‐related molecules and pathways in B lymphocytes, highlights the most relevant techniques to ...
Marta Sampietro   +2 more
wiley   +1 more source

Polyacrylamide gel electrophoresis of soybean seed proteins

open access: yesActa Societatis Botanicorum Poloniae, 2015
Four major and 14 minor protein bands were detected when total salt soluble proteins of soybean (Glycine max cultivar Warszawska) seed were subjected to polyacrylamide gel electrophoresis under nondissociating conditions, and 16 protein bands were ...
W. Lassocińsk, J. S. Knypl
doaj   +1 more source

Home - About - Disclaimer - Privacy