Results 251 to 260 of about 446,459 (290)
Some of the next articles are maybe not open access.
Polyacrylamide Gel Electrophoresis
Science, 1971Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over ...
A, Chrambach, D, Rodbard
openaire +2 more sources
Oriented macroporous polyacrylamide gels
ELECTROPHORESIS, 1997AbstractMacroporous gels with huge cavities and partition walls result from controlled microsyneresis during gelation. In this report we show that the microsyneresis process can be further controlled: it is possible to orient the partition walls of macropores by the use of an electric field throughout polymerization.
R, Charlionet +3 more
openaire +2 more sources
2012
Usually proteins are separated by polyacrylamide gel electrophoresis (PAGE) in the presence of a detergent and under (heat-) denaturing and (non- or) reducing conditions. The most commonly used detergent is sodium dodecyl sulfate (SDS). The major function of SDS is to shield the respective charge of the proteins present in the mixture to be analyzed ...
Claudia, Arndt +3 more
openaire +2 more sources
Usually proteins are separated by polyacrylamide gel electrophoresis (PAGE) in the presence of a detergent and under (heat-) denaturing and (non- or) reducing conditions. The most commonly used detergent is sodium dodecyl sulfate (SDS). The major function of SDS is to shield the respective charge of the proteins present in the mixture to be analyzed ...
Claudia, Arndt +3 more
openaire +2 more sources
Electroblotting from Polyacrylamide Gels
Current Protocols in Protein Science, 1995AbstractThis unit contains procedures for electrophoretically transferring proteins onto a variety of membranes including polyvinylidene difluoride (PVDF) and nitrocellulose, and derivatized membranes. The choice of membrane type for electrotransfer is dependent on the ultimate application for the blot membrane.
J A, Ursitti +2 more
openaire +2 more sources
2018
Proteins can easily be separated by polyacrylamide gel electrophoresis (PAGE) in the presence of a detergent and under (heat-) denaturing and (non- or) reducing conditions. The most commonly used detergent is sodium dodecyl sulfate (SDS). The major function of SDS is to shield the respective charge of the proteins present in the mixture to be analyzed ...
Arndt, C. +3 more
openaire +3 more sources
Proteins can easily be separated by polyacrylamide gel electrophoresis (PAGE) in the presence of a detergent and under (heat-) denaturing and (non- or) reducing conditions. The most commonly used detergent is sodium dodecyl sulfate (SDS). The major function of SDS is to shield the respective charge of the proteins present in the mixture to be analyzed ...
Arndt, C. +3 more
openaire +3 more sources
Drying SDS-Polyacrylamide Gels
Cold Spring Harbor Protocols, 2006INTRODUCTIONThis protocol describes a method for drying SDS-polyacrylamide gels. Gels containing proteins radiolabeled with 35S-labeled amino acids must be dried before autoradiographic images can be obtained. Nonradioactive gels can also be preserved by drying.
Joseph, Sambrook, David W, Russell
openaire +2 more sources
Fluorometric scanning of polyacrylamide gels
Analytical Biochemistry, 1969Abstract A gel scanning technique that measures the quench of N-allyl-DANSA fluorescence by amido schwarz stained protein bands had the potential capability of about fifty times the sensitivity of absorption densitometric techniques. The preparation of the fluorescent polyacrylamide gels was straightforward and their electrophoretic resolving and ...
D P, Borris, J N, Aronson
openaire +2 more sources
High concentration polyacrylamide gel electrophoresis
Analytical Biochemistry, 1986A simple technique is described for easy removal of high concentration polyacrylamide gels after electrophoresis from glass tubes without breaking them.
V S, Dhamankar +2 more
openaire +2 more sources
Rapid Destaining of Polyacrylamide Gel
Nature, 1966WITH increasing use of polyacrylamide gel in zone-electrophoresis, the need for rapid removal of excess stain from the gel increases. The conventional destaining of the gel by washing in 5–7 per cent acetic acid may take from one to several days. This is not only inconvenient but also renders this technique unsuitable for the purification of biological
L K, Nagy, B, Rogerson, N, Tomkuss
openaire +2 more sources
Improvements in polyacrylamide gel slicing
Analytical Biochemistry, 1977Abstract An improved polyacrylamide gel slicer has been devised that provides rapid uniform slicing with a precision of 4–6%. The advantages of this type of slicer are: The gel is sliced directly from the electrophoresis tube; gel diameter and length can vary with no modification of the system; and gels with a range of acrylamide concentrations can ...
openaire +2 more sources