Results 271 to 280 of about 431,758 (295)

Detection of oxidases on polyacrylamide gels

Analytical Biochemistry, 1973
Abstract Direct transfer of electrons, from the reduced flavin moiety of several oxidases to phenazine methosulfate and then to nitroblue tetrazolium, serves to detect activity of the oxidases of l -amino acids, urate, diamines, hydroxy acids (both long- and short-chain), aldehydes, and sarcosine.
Ronald Lindahl, Robert N. Feinstein
openaire   +3 more sources

Improvements in polyacrylamide gel slicing

Analytical Biochemistry, 1977
Abstract An improved polyacrylamide gel slicer has been devised that provides rapid uniform slicing with a precision of 4–6%. The advantages of this type of slicer are: The gel is sliced directly from the electrophoresis tube; gel diameter and length can vary with no modification of the system; and gels with a range of acrylamide concentrations can ...
openaire   +3 more sources

Electroblotting from Polyacrylamide Gels

Current Protocols in Protein Science, 1995
AbstractThis unit contains procedures for electrophoretically transferring proteins onto a variety of membranes including polyvinylidene difluoride (PVDF) and nitrocellulose, and derivatized membranes. The choice of membrane type for electrotransfer is dependent on the ultimate application for the blot membrane.
J A, Ursitti, J, Mozdzanowski, D W, Speicher   +2 more
openaire   +2 more sources

Degradation on polyacrylamides. Part II. Polyacrylamide gels

Polymer, 2003
Abstract The stability of polyacrylamide (PAAm) gels, synthesized by free radical polymerization of acrylamide (AAm) and N , N ′-methylenebisacrylamide (BIS), was investigated when subjected to thermal and irradiation conditions. The PAAm gels were stable and did not release AAm under fluorescent light.
Xiaojuan Hao, David H. Solomon, Greg G. Qiao, Marcus J. Caulfield   +3 more
openaire   +2 more sources

Isolation of Proteins from Polyacrylamide Gels

2012
Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.
Irene Michalk, Stefanie Koristka, Claudia Arndt, Michael Bachmann   +3 more
openaire   +4 more sources

Silver Staining Techniques of Polyacrylamide Gels

2012
After SDS-polyacrylamide gel electrophoresis the separated proteins have to be visualized by staining the gel. The same is true after transfer of separated proteins to a blotting membrane in order to verify an efficient transfer and to visualize the amount of protein(s) remaining in the gel.
Michael Bachmann, Holger Bartsch, Marc Cartellieri, Claudia Arndt, Stefanie Koristka   +4 more
openaire   +4 more sources

Micro-Electrophoresis on Polyacrylamide Gels

1973
Polyacrylamide gels were introduced in 1959 by Raymond and Weintraub, as supports for electrophoretic separations. The polyacrylamide gel is produced by polymerising acrylamide, with N,N-methylenebisacrylamide or ethylene diacrylate as the cross-linking component. Catalytic redox systems, which yield free radicals, are used to initate copolymerisation (
openaire   +3 more sources

SDS Polyacrylamide Gel Electrophoresis of Proteins

2003
Probably the most widely used of techniques for analyzing mixtures of proteins is SDS polyacrylamide gel electrophoresis. In this technique, proteins are reacted with the anionic detergent, sodium dodecylsulfate (SDS, or sodium lauryl sulfate) to form negatively charged complexes.
openaire   +4 more sources

ISOELECTRIC FOCUSING IN POLYACRYLAMIDE GELS

The Lancet, 1968
G. Dale, A.L. Latner
openaire   +3 more sources

Microelectrophoresis on polyacrylamide gel

Biochimica et Biophysica Acta (BBA) - Protein Structure, 1967
openaire   +2 more sources