Results 21 to 30 of about 431,758 (295)

Tetra-gel enables superior accuracy in combined super-resolution imaging and expansion microscopy

Scientific Reports, 2021
The accuracy of expansion microscopy (ExM) depends on the structural preservation of samples embedded in a hydrogel. However, it has been unknown to what extent gel embedding alters the molecular positions of individual labeled sites. Here, we quantified
Hsuan Lee, Chih-Chieh Yu, Edward S. Boyden, Xiaowei Zhuang, Pallav Kosuri   +4 more
doaj   +1 more source

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Physicochemical properties of free and immobilized tyrosinase from different species of yam (Dioscorea spp)

Biotechnology Reports, 2020
A shortened method of purification and immobilization of tyrosinase from different species of yam (Dioscorea spp) on insoluble supports is described.
Olutosin Samuel Ilesanmi, Isaac Olusanjo Adewale   +1 more
doaj  

Electroblotting from Polyacrylamide Gels [PDF]

Current Protocols in Protein Science, 2015
AbstractTransferring proteins from polyacrylamide gels onto retentive membranes is now primarily used for immunoblotting. A second application that was quite common up to about a decade ago was electroblotting of proteins for N‐terminal and internal sequencing using Edman chemistry.
Jacek Mozdzanowski, David W. Speicher, Jeanine A. Ursitti, Aaron R. Goldman   +3 more
openaire   +3 more sources

Disruption of SETD3‐mediated histidine‐73 methylation by the BWCFF‐associated β‐actin G74S mutation

FEBS Letters, EarlyView.
The β‐actin G74S mutation causes altered interaction of actin with SETD3, reducing histidine‐73 methylation efficiency and forming two distinct actin variants. The variable ratio of these variants across cell types and developmental stages contributes to tissue‐specific phenotypical changes. This imbalance may impair actin dynamics and mechanosensitive
Anja Marquardt, Marcus S. Münchhoff, Jacqueline Krohn, Philip M. Palarz, Manuel H. Taft, Johannes N. Greve, Nataliya Di Donato, Falk F. R. Buettner, Dietmar J. Manstein   +8 more
wiley   +1 more source

Intron‐oriented HTLV‐1 integration in an adult T‐cell leukemia/lymphoma cell line sustains expression of intact ift81 mRNA

FEBS Letters, EarlyView.
In the adult T‐cell leukemia/lymphoma (ATL) cell line ED, the human T‐cell leukemia virus type 1 (HTLV‐1) provirus was integrated into the intron of the ift81 gene in the antisense orientation. Despite this integration, both the intact ift81 and the viral oncogene hbz were simultaneously expressed, likely due to the functional insufficiency of viral ...
Mayuko Yagi, Yuka Hirosawa, Yusaku Sagisaka, Minami Hama, Satoshi Takeda, Jun A. Komano   +5 more
wiley   +1 more source

High-Performance Anti-freezing Flexible Zn-MnO2 Battery Based on Polyacrylamide/Graphene Oxide/Ethylene Glycol Gel Electrolyte

Frontiers in Chemistry, 2020
It remains a great challenge for aqueous zinc-ion batteries to work at subzero temperatures, since the water in aqueous electrolytes would freeze and inhibit the transportation of electrolyte ions, inevitably leading to performance deterioration. In this
Yuhui Quan, Minfeng Chen, Weijun Zhou, Qinghua Tian, Jizhang Chen, Jizhang Chen   +5 more
doaj   +1 more source

A simple method of drying polyacrylamide slab gels that eliminates cracking

BioTechniques, 2021
The polyacrylamide slab gel is the most common gel format for analyzing protein samples by electrophoresis. Drying these gels is useful in many biological applications; for example, autoradiography, in which radiolabeled proteins are separated to enable ...
Ghanshyam D Heda
doaj   +1 more source

ShcD adaptor protein drives invasion of triple negative breast cancer cells by aberrant activation of EGFR signaling

Molecular Oncology, EarlyView.
We identified adaptor protein ShcD as upregulated in triple‐negative breast cancer and found its expression to be correlated with reduced patient survival and increased invasion in cell models. Using a proteomic screen, we identified novel ShcD binding partners involved in EGFR signaling pathways.
Hayley R. Lau, Hayley S. Smith, Begüm Alural, Claire E. Martin, Laura A. New, Manali Tilak, Sara L. Banerjee, Hannah N. Robeson, Nicolas Bisson, Anne‐Claude Gingras, Jasmin Lalonde, Nina Jones   +11 more
wiley   +1 more source

Urea Delays High-Temperature Crosslinking of Polyacrylamide for In Situ Preparation of an Organic/Inorganic Composite Gel

Gels
To address the rapid crosslinking reaction and short stability duration of polyacrylamide gel under high salinity and temperature conditions, this paper proposes utilizing urea to delay the nucleophilic substitution crosslinking reaction among ...
Li Liang, Junlong Li, Dongxiang Li, Jie Xu, Bin Zheng, Jikuan Zhao   +5 more
doaj   +1 more source