Results 151 to 160 of about 920,072 (304)

Therapeutic Gene Editing of APOE4 in Sporadic Alzheimer's Disease via Prime Editor 7

Advanced Science, EarlyView.
Prime Editor 7‐mediated conversion of APOE4 to APOE3 alleviates Alzheimer's disease‐associated pathology in AD mouse models and patient‐derived neurons and improves cognitive performance in vivo, supporting therapeutic genome editing as a promising strategy for APOE4‐associated neurodegeneration.
Yunkyung Kim, Gaeun Lee, Saemin An, Hanseul Park, Hongwon Kim, Jisung Kim, Soi Kang, Sumin Kim, Daeyeol Kwon, Jeonghyun Park, Yerim Hwang, Seonghun Kim, Xiuwen Yuan, Jaehoon Jeong, Hamin Lee, Hui Kwon Kim, Jongpil Kim   +16 more
wiley   +1 more source

Targeted Degradation of Picornaviral 3C Protease via PROTACs Confers High Barrier to Viral Resistance and Broad‐Spectrum Antiviral Activity

Advanced Science, EarlyView.
This study reports D34 as the first PROTAC degrader that targets the 3C protease of picornaviruses. D34 effectively degrades EV71 3C protease via the ubiquitin‐proteasome pathway. More importantly, D34 exhibits a high resistance barrier and shows broad‐spectrum antiviral activity against multiple picornaviruses, highlighting its potential as a novel ...
Weilong Deng, Junyu Chen, Yingyue Pang, Yuanyuan Zhang, Siqian Chen, Guoliang You, Xiaoman Tian, Zhongxin Xu, Jing Wang, Luqing Shang   +9 more
wiley   +1 more source

Improved sodium dodecyl sulfate-polyacrylamide gel electrophoresis method for quantifying the 11S/7S ratio in soybean storage proteins. [PDF]

J Sci Food Agric
Zheng Y, Denbow C, do Granja MO, Pilot G, Wang Z, Rosso ML, Zhang B.   +6 more
europepmc   +1 more source

DNA ladders can be used to size polyphosphate resolved by polyacrylamide gel electrophoresis. [PDF]

Electrophoresis, 2018
Smith SA, Wang Y, Morrissey JH.
europepmc   +1 more source

A Universal Protein Ladder for Standardization of Diverse FRET Assays

Advanced Science, EarlyView.
Translating FRET measurements from in vitro to intracellular environments requires universal benchmarks. We present a modular protein ladder using TPR motifs to harmonize data across diverse platforms. This predictable calibration curve bridges single molecule FRET measurements, with cell based measurements including FLIM‐FRET.
Evelyn R. Smith, Katie L. Gelder, Lewis Hunter‐Craig, Daniel A. Bose, Timothy D. Craggs, Alison E. Twelvetrees   +5 more
wiley   +1 more source

POLYACRYLAMIDE-GEL ELECTROPHORESIS OF WHOLE PROTEINS FROM ORAL STREPTOCOCCUS STRAINS

, 2015
As a contribution to the identification and characterization of oral Streptococcus, culture samples of the mutans group, biochemically identified as S. mutans, S.
SPOLIDORIO, DMP, VERLENGIA, R, HOFLING, JF   +2 more
core  

Analysis of proteins by a radical-free and highly reducing method of two-dimensional polyacrylamide gel electrophoresis. [PDF]

Front Mol Biosci
Wada A, Ueta M, Wada C, Maki Y, Tanzawa T, Yoshida H.   +5 more
europepmc   +1 more source

Controlling Dispersion during Single-Cell Polyacrylamide-Gel Electrophoresis in Open Microfluidic Devices. [PDF]

Anal Chem, 2018
Pan Q, Yamauchi KA, Herr AE.
europepmc   +1 more source

Identifying a Csmd3+ Microglial Subpopulation that Drives Cold‐to‐Hot Transition and Immune‐Cure in Glioblastoma

Advanced Science, EarlyView.
The study establishes an immune‐cure (ICu) mouse model based on a TMEMed G422TN‐GBM system that faithfully recapitulates human TMEMed GBM. scRNA‐seq analysis reveals a Csmd3+ microglial subset with innate immune memory (IIM) potential that potently suppresses GBM growth, drives a TME cold‐to‐hot transition, and induces 100% ICu in long‐term survival ...
Hai‐Feng Jiang, Pan‐Pan Gao, Yu‐Wen Du, Li‐Qin Wu, En‐Zhi Yin, Qi An, Ze‐Hua Ding, Jin‐Wen Shi, Ya Shu, Ruoqiao Chen, Feng Liu, Mingfeng Li, Xiao Qian Chen   +12 more
wiley   +1 more source

Analysis of Protein Binding to Liver Mitochondria by Horizontal Polyacrylamide Gel Electrophoresis Using a Converted Agarose Electrophoresis Unit. [PDF]

J Clin Transl Hepatol
Thakkar B, Wu GY.
europepmc   +1 more source