Results 301 to 310 of about 523,208 (360)
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Polyacrylamide Gel Electrophoresis
Science, 1971Polyacrylamide gel electrophoresis (PAGE) provides a versatile, gentle, high resolution method for fractionation and physical-chemical characterization of molecules on the basis of size, conformation, and net charge. The polymerization reaction can be rigorously controlled to provide uniform gels of reproducible, measurable pore size over ...
A, Chrambach, D, Rodbard
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ELECTROPHORESIS OF HISTONES ON POLYACRYLAMIDE GELS
Canadian Journal of Biochemistry and Physiology, 1963Histories extracted from various organs of the rat have been fractionated by electrophoresis on Polyacrylamide gels. The most convenient reagents to form the gel were selected and the effects of varying concentrations of these reagents on the resolution of histones were investigated.
A, DRIEDGER, L D, JOHNSON, A M, MARKO
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C3 Polymorphism in Polyacrylamide‐Gel Electrophoresis
Vox Sanguinis, 1976Abstract. C3 polymorphism in polyacrylamide‐gel electrophoresis was identified by crossed immunoelectrophoresis using anti‐C3/C3c‐serum. Through ageing, treatment of sera with cobra venom factor, endotoxin or with neuraminidase, polymorphic bands were seen also in a conversion product and antigenically attributed to C3c.
J, Schwamborn, H, Freis, G, Mauff
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Polyacrylamide Gel Electrophoresis of Lysates of Mycoplasmas
Research in Veterinary Science, 1970The technique of Razin & Rottem (1967) for electrophoretic comparison of mycoplasmas has been modified, principally by carrying out the electrophoresis on flat gel slabs, and has been used to examine lysates of several mycoplasma preparations. The 6 different species compared could readily be distinguished, while different batches and isolates of the ...
H, Zola, W, Baxendale, L J, Sayer
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Polyacrylamide gel electrophoresis on micro slabs
Analytical Biochemistry, 1972Abstract An electrophoretic technique using micro polyacrylamide flat gels is described and its usefulness demonstrated. The gels are vertically cast and electrophoresed in slab form (75 × 18 × 0.75 mm) in closed thin glass cells (cuvets) made from detachable microscope slides.
H R, Maurer, F A, Dati
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Micro-Electrophoresis on Polyacrylamide Gels
1973Polyacrylamide gels were introduced in 1959 by Raymond and Weintraub, as supports for electrophoretic separations. The polyacrylamide gel is produced by polymerising acrylamide, with N,N-methylenebisacrylamide or ethylene diacrylate as the cross-linking component. Catalytic redox systems, which yield free radicals, are used to initate copolymerisation (
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High concentration polyacrylamide gel electrophoresis
Analytical Biochemistry, 1986A simple technique is described for easy removal of high concentration polyacrylamide gels after electrophoresis from glass tubes without breaking them.
V S, Dhamankar +2 more
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‘Laterally aggregated’ polyacrylamide gels for electrophoresis
ELECTROPHORESIS, 1992AbstractA new method is described for producing highly porous polyacrylamide matrices: polymerization in presence of a preformed hydrophilic polymer. If a standard mixture of monomers (e.g., 5%T, 4%C) is polymerized in presence of, e.g., polyethylene glycol (PEG) 10 kDa, lateral chain aggregation occurs, with formation of large pore sizes.
Righetti P. G. +3 more
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SDS – polyacrylamide gel electrophoresis of lipopolysaccharides
Canadian Journal of Microbiology, 1975Lipopolysaccharides (LPS) prepared from four different species of Neisseria have been separated by SDS – polyacrylamide gel electrophoresis. Each LPS possessed a characteristic mobility on gels. Examination of the effect of acrylamide concentration on migration illustrated that the basis of the separation was molecular size, and not intrinsic charge.
R R, Russell, K G, Johnson
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A miniaturized system for electrophoresis on polyacrylamide gels
Analytical Biochemistry, 1979The design, construction, and use of a simple economical system for gel electrophoresis are described, along with effective procedures and the required materials. Examples of successful use of the system are presented.
Z I, Ogita, C L, Markert
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