Results 1 to 10 of about 1,273,483 (249)
Frontiers in Bioengineering and Biotechnology, 2021 A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter.
Wayne M. Barnes +3 more
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A new contribution to Megasporoporia sensu lato: Six new species and three new combinations
Frontiers in Microbiology, 2022 Megasporoporia sensu lato has recently been intensively studied in China and South America, and four independent clades representing four genera have been recognized phylogenetically. In this study, more samples, mostly from subtropical and tropical Asia,
Ya-Rong Wang +7 more
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Viruses, 2021 Viruses have evolved numerous strategies to maximize the use of their limited genetic material, including proteolytic cleavage of polyproteins to yield products with different functions.
Dennis S. Winston, David D. Boehr
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Beyond the Lesion: Back to High Fidelity DNA Synthesis
Frontiers in Molecular Biosciences, 2022 High fidelity (HiFi) DNA polymerases (Pols) perform the bulk of DNA synthesis required to duplicate genomes in all forms of life. Their structural features, enzymatic mechanisms, and inherent properties are well-described over several decades of research.
Joseph D. Kaszubowski +1 more
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LigD: A Structural Guide to the Multi-Tool of Bacterial Non-Homologous End Joining
Frontiers in Molecular Biosciences, 2021 DNA double-strand breaks are the most lethal form of damage for living organisms. The non-homologous end joining (NHEJ) pathway can repair these breaks without the use of a DNA template, making it a critical repair mechanism when DNA is not replicating ...
Benhur Amare +10 more
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Modified Nucleic Acids: Expanding the Capabilities of Functional Oligonucleotides
Molecules, 2020 In the last three decades, oligonucleotides have been extensively investigated as probes, molecular ligands and even catalysts within therapeutic and diagnostic applications.
Steven Ochoa, Valeria T. Milam
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Viruses, 2020 Current influenza vaccines have modest efficacy. This is especially true for current live attenuated influenza vaccines (LAIV), which have been inferior to the inactivated versions in recent years.
Andrew Cox +7 more
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Versatile SARS-CoV-2 Reverse-Genetics Systems for the Study of Antiviral Resistance and Replication
Viruses, 2022 The COVID-19 pandemic continues to threaten healthcare systems worldwide due to the limited access to vaccines, suboptimal treatment options, and the continuous emergence of new and more transmissible SARS-CoV-2 variants.
Ulrik Fahnøe +11 more
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Virulence, 2021 HBV pol plays a critical role in the replication of hepatitis B virus (HBV). Previous studies conducted on HBV pol have produced limited evidence on HBV pol expression due to the lack of effective detection methods.
Song Hu +6 more
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Open Biology, 2023 The enterobacterial common antigen (ECA) is a carbohydrate polymer that is associated with the cell envelope in the Enterobacteriaceae. ECA contains a repeating trisaccharide which is polymerized by WzyE, a member of the Wzy membrane protein polymerase ...
Miriam Weckener +8 more
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