Results 1 to 10 of about 2,158,859 (241)

Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors [PDF]

Frontiers in Bioengineering and Biotechnology
We present a live culture PCR (LC-PCR) workflow that enables direct screening of randomly mutagenized Thermus aquaticus (Taq) and Klentaq1 DNA polymerase libraries without enzyme purification.
Milko B. Kermekchiev, Zhian Zhang, Wayne M. Barnes, Wayne M. Barnes   +3 more
doaj   +2 more sources

A Single Amino Acid Change to Taq DNA Polymerase Enables Faster PCR, Reverse Transcription and Strand-Displacement

Frontiers in Bioengineering and Biotechnology, 2021
A change of an aspartic acid to asparagine of Taq (Thermus aquaticus) DNA polymerase is a gain of function mutation that supports faster PCR: the extension times for PCR amplification can be 2–3 times shorter.
Wayne M. Barnes, Wayne M. Barnes, Zhian Zhang, Milko B. Kermekchiev   +3 more
doaj   +1 more source

The lipid linked oligosaccharide polymerase Wzy and its regulating co-polymerase, Wzz, from enterobacterial common antigen biosynthesis form a complex

Open Biology, 2023
The enterobacterial common antigen (ECA) is a carbohydrate polymer that is associated with the cell envelope in the Enterobacteriaceae. ECA contains a repeating trisaccharide which is polymerized by WzyE, a member of the Wzy membrane protein polymerase ...
Miriam Weckener, Laura S. Woodward, Bradley R. Clarke, Huanting Liu, Philip N. Ward, Audrey Le Bas, David Bhella, Chris Whitfield, James H. Naismith   +8 more
doaj   +1 more source

Chronic Hepatitis B Treatment Strategies Using Polymerase Inhibitor-Based Combination Therapy

Viruses, 2021
Viral polymerase is an essential enzyme for the amplification of the viral genome and is one of the major targets of antiviral therapies. However, a serious concern to be solved in hepatitis B virus (HBV) infection is the difficulty of eliminating ...
Eriko Ohsaki, Yadarat Suwanmanee, Keiji Ueda   +2 more
doaj   +1 more source

DNA Helicase–Polymerase Coupling in Bacteriophage DNA Replication

Viruses, 2021
Bacteriophages have long been model systems to study the molecular mechanisms of DNA replication. During DNA replication, a DNA helicase and a DNA polymerase cooperatively unwind the parental DNA.
Chen-Yu Lo, Yang Gao
doaj   +1 more source

Production of Bst polymerase for diagnosis of different infections using loop-mediated isothermal amplification

Журнал микробиологии, эпидемиологии и иммунобиологии, 2023
Introduction. The large fragment of DNA polymerase I from Geobacillus stearothermophilus GIM1.543 (Bst DNA polymerase) possesses 5'-3' DNA polymerase activity, 5'-3' displacement activity and high processivity.
Maria I. Pika, Olga O. Mikheeva, Elena D. Solovyova, Anna V. Valdokhina, Victoria P. Bulanenko, Evgeny A. Cherkashin, Vadim V. Petrov, Kirill V. Krasovitov, Anna S. Cherkashina, Vasily G. Akimkin   +9 more
doaj   +1 more source

Mobile genetic element-encoded putative DNA primases composed of A-family polymerase—SSB pairs

Frontiers in Molecular Biosciences, 2023
Mobile genetic elements can encode a wide variety of genes that support their own stability and mobility as well as genes that provide accessory functions to their hosts.
Phoebe A. Rice
doaj   +1 more source

Prophylaxis and treatment of influenza: options, antiviral susceptibility, and existing recommendations

GMS Infectious Diseases, 2021
Influenza viruses of types A and B attack 5–10% of adults and 20–30% of children, thereby causing millions of acute respiratory infections in Germany annually.
Duwe, Susanne C., Schmidt, Barbara, Gärtner, Barbara C., Timm, Jörg, Adams, Ortwin, Fickenscher, Helmut, Schmidtke, Michaela   +6 more
doaj   +1 more source

Aurintricarboxylic Acid Suppresses Hepatitis B Virus Replication by Inhibition of RNase H Activity

Frontiers in Virology, 2022
BackgroundChronic hepatitis B virus (HBV) infection is a global health threat for which there is an urgent need to develop novel therapeutics.MethodsAurintricarboxylic acid (ATA) has been demonstrated with broad-spectrum antimicrobial and antiviral ...
Meng Xu, Xiao Guan
doaj   +1 more source

Phenotypic Characterization of Sulfolobus islandicus Strains Lacking the B-Family DNA Polymerases PolB2 and PolB3 Individually and in Combination

Frontiers in Microbiology, 2021
Across the three domains of life, B-family DNA polymerases play a variety of roles in both DNA repair and DNA replication processes. We examine the phenotypic consequences of loss of the putative repair polymerases PolB2 and/or PolB3 in the crenarchaeon ...
Peter B. Bohall, Peter B. Bohall, Stephen D. Bell, Stephen D. Bell   +3 more
doaj   +1 more source