Results 101 to 110 of about 2,105,688 (341)
DNA Polymerase Profiling [PDF]
, 2008 We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template strand. A resulting distance alteration is reported by fluorescence resonance energy transfer between two dyes ...
openaire +3 more sources
Replicative DNA Polymerases [PDF]
Cold Spring Harbor Perspectives in Biology, 2013 In 1959, Arthur Kornberg was awarded the Nobel Prize for his work on the principles by which DNA is duplicated by DNA polymerases. Since then, it has been confirmed in all branches of life that replicative DNA polymerases require a single-stranded template to build a complementary strand, but they cannot start a new DNA strand de novo.
Johansson, Erik, Dixon, Nicholas
openaire +4 more sources
Polyprotein Processing as a Determinant for in Vitro Activity of Semliki Forest Virus Replicase
Viruses, 2017 Semliki Forest virus (SFV) is an arthropod-borne alphavirus that induces membrane invaginations (spherules) in host cells. These harbor the viral replication complexes (RC) that synthesize viral RNA.
Maija K. Pietilä +3 more
doaj +1 more source
Transcriptome‐wide analysis of circRNA and RBP profiles and their molecular relevance for GBM
Molecular Oncology, EarlyView.CircRNAs are differentially expressed in glioblastoma primary tumors and might serve as therapeutic targets and diagnostic markers. The investigation of circRNA and RNA‐binding proteins (RBPs) interactions shows that distinct RBPs play a role in circRNA biogenesis and function.
Julia Latowska‐Łysiak +14 more
wiley +1 more source
Spt5 Cooperates with Human Immunodeficiency Virus Type 1 Tat by Preventing Premature RNA Release at Terminator Sequences [PDF]
, 2002 The human immunodeficiency virus type 1 (HIV-1) Tat protein activates transcription elongation by stimulating the Tat-activated kinase (TAK/p-TEFb), a protein kinase composed of CDK9 and its cyclin partner, cyclin T1.
Bourgeois, Cyril F +4 more
core +3 more sources
Molecular Oncology, EarlyView.This study investigates an alternative approach to reactivating the oncosuppressor p53 in cancer. A short peptide targeting the association of the two p53 inhibitors, MDM2 and MDM4, induces an otherwise therapeutically active p53 with unique features that promote cell death and potentially reduce toxicity towards proliferating nontumor cells.
Sonia Valentini +10 more
wiley +1 more source
Directed polymerase evolution [PDF]
FEBS Letters, 2013 Polymerases evolved in nature to synthesize DNA and RNA, and they underlie the storage and flow of genetic information in all cells. The availability of these enzymes for use at the bench has driven a revolution in biotechnology and medicinal research; however, polymerases did not evolve to function efficiently under the conditions required for some ...
Floyd E. Romesberg, Tingjian Chen
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Rescue of Synthetic Genomic RNA Analogs of Rabies Virus by Plasmid-Encoded Proteins [PDF]
, 1994 Proteins eolirely expressed from cDNA wen used to rescue synthetic RNA genome analogs into infectious defective particles or rabies virus (RV). Synthetic negative-stranded RNAs coßtalning 3' · and S'-terminal RV sequences and tnlßscriptional ...
Conzelmann, Karl-Klaus, Schnell, M.-J.
core +3 more sources
Human PrimPol is a highly error-prone polymerase regulated by single-stranded DNA binding proteins [PDF]
, 2014 PrimPol is a recently identified polymerase involved in eukaryotic DNA damage tolerance, employed in both re-priming and translesion synthesis mechanisms to bypass nuclear and mitochondrial DNA lesions.
Aaron Ehlinger +55 more
core +1 more source
Bioplastics and food packaging: A review
Cogent Food & Agriculture, 2015 Food packaging as a vital part of the subject of food technology is involved with protection and preservation of all types of foods. Due to economical abundance, petrochemical plastics have been largely used as packaging material due to their desirable ...
Nafisa Jabeen +2 more
doaj +1 more source