Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction.
BioTechniques, 2013 Gene Splicing by Overlap Extension or "gene SOEing" is a PCR-based method of recombining DNA sequences without reliance on restriction sites and of directly generating mutated DNA fragments in vitro.
R. Horton, Z. Cai, S. Ho, L. Pease
semanticscholar +1 more source
Spt5 Cooperates with Human Immunodeficiency Virus Type 1 Tat by Preventing Premature RNA Release at Terminator Sequences [PDF]
, 2002 The human immunodeficiency virus type 1 (HIV-1) Tat protein activates transcription elongation by stimulating the Tat-activated kinase (TAK/p-TEFb), a protein kinase composed of CDK9 and its cyclin partner, cyclin T1.
Bourgeois, Cyril F +4 more
core +3 more sources
A critical survey on the kinetic assays of DNA polymerase fidelity from a new theoretical perspective [PDF]
arXiv, 2020 The high fidelity of DNA polymerase is critical for the faithful replication of genomic DNA. Several approaches were proposed to quantify the fidelity of DNA polymerase. Direct measurements of the error frequency of the replication products definitely give the true fidelity but turn out very hard to implement.
arxiv
Uncovering the effect of RNA polymerase steric interactions on gene expression noise: analytical distributions of nascent and mature RNA numbers [PDF]
arXiv, 2023 The telegraph model is the standard model of stochastic gene expression, which can be solved exactly to obtain the distribution of mature RNA numbers per cell. A modification of this model also leads to an analytical distribution of the nascent RNA numbers. These solutions are routinely used for the analysis of single-cell data, including the inference
arxiv
Poly(A) Polymerase and Poly(G) Polymerase in Wheat Chloroplasts [PDF]
Proceedings of the National Academy of Sciences, 1974 Extracts of wheat chloroplasts contain a poly(A) polymerase which can polymerize AMP residues from ATP onto an RNA primer. Whole extracts of wheat leaves also contain another poly(A) polymerase which is present in much larger amount and is probably derived from the nuclei.
Gerard Burkard, Elizabeth B. Keller
openaire +3 more sources
Structure and mechanism of human DNA polymerase η [PDF]
, 2010 The variant form of the human syndrome xeroderma pigmentosum (XPV) is caused by a deficiency in DNA polymerase eta (Pol eta), a DNA polymerase that enables replication through ultraviolet-induced pyrimidine dimers.
A Alt +66 more
core +2 more sources
Recombinase Polymerase Amplification for Diagnostic Applications
Clinical Chemistry, 2016 BACKGROUND First introduced in 2006, recombinase polymerase amplification (RPA) has stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them just in the last 2 years. The widespread adoption of this isothermal molecular
Rana K. Daher +3 more
semanticscholar +1 more source
Rescue of Synthetic Genomic RNA Analogs of Rabies Virus by Plasmid-Encoded Proteins [PDF]
, 1994 Proteins eolirely expressed from cDNA wen used to rescue synthetic RNA genome analogs into infectious defective particles or rabies virus (RV). Synthetic negative-stranded RNAs coßtalning 3' · and S'-terminal RV sequences and tnlßscriptional ...
Conzelmann, Karl-Klaus, Schnell, M.-J.
core +3 more sources
PrimPol is required for replicative tolerance of G quadruplexes in vertebrate cells [PDF]
, 2016 G quadruplexes (G4s) can present potent blocks to DNA replication. Accurate and timely replication of G4s in vertebrates requires multiple specialized DNA helicases and polymerases to prevent genetic and epigenetic instability.
Bailey, Laura J +7 more
core +1 more source
A Comprehensive Superposition of Viral Polymerase Structures
Viruses, 2019 Nucleic acid polymerases are essential enzymes that replicate the genomes of both RNA and DNA viruses. These enzymes are generally encoded by viruses themselves so as to provide biochemical functions and control elements that differ from those of the ...
Olve B. Peersen
doaj +1 more source