Results 171 to 180 of about 2,178,231 (341)

Atm deficiency in the DNA polymerase β null cerebellum results in cerebellar ataxia and Itpr1 reduction associated with alteration of cytosine methylation [PDF]

, 2020
Jusik Kim, Keeeun Kim, Jung‐Soon Mo, Youngsoo Lee   +3 more
openalex   +1 more source

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

A high-throughput screen identifies that CDK7 activates glucose consumption in lung cancer cells. [PDF]

, 2019
Elevated glucose consumption is fundamental to cancer, but selectively targeting this pathway is challenging. We develop a high-throughput assay for measuring glucose consumption and use it to screen non-small-cell lung cancer cell lines against ...
Chen, Bao Ying, Clark, Peter M, Damoiseaux, Robert, Ghezzi, Chiara, Ribalet, Bernard, Wong, Alicia   +5 more
core   +1 more source

Conserved functions of the trigger loop and Gre factors in RNA cleavage by bacterial RNA polymerases [PDF]

, 2017
Nataliya Miropolskaya, Daria Esyunina, Andrey Kulbachinskiy   +2 more
openalex   +1 more source

Domain associated with zinc fingers‐containing NF90‐NF45 complex inhibits m6A modification of primary microRNA by suppressing METTL3/14 activity

FEBS Open Bio, EarlyView.
NF90–NF45 functions as a negative regulator of methyltransferase‐like 3/14 (METTL3/14)‐mediated N6‐methyladenosine (m6A) modification on primary microRNAs (pri‐miRNAs). NF90–NF45 binds to anti‐oncogenic pri‐miRNAs and inhibits their m6A modification, thereby suppressing the biogenesis of anti‐oncogenic miRNAs.
Takuma Higuchi, Shunsuke Morioka, Keiko Morisawa, Kazutsugu Matsukawa, Shingo Ashida, Takeshi Suzuki, Shuji Sakamoto   +6 more
wiley   +1 more source

Pioglitazone plus (−)‐epigallocatechin gallate: a novel approach to enhance osteogenic performance in aged bone marrow mesenchymal stem cells

FEBS Open Bio, EarlyView.
Aged human bmMSCs are seeded in the scaffold. Osteoblastic induction can slightly increase cell's bone‐forming activity to produce bone‐like tissues, shown as the sporadic xylenol orange‐stained spots (the lower left image). Notably, pioglitazone plus EGCG co‐treatment dramatically increases cell's bone‐forming activity and bone‐like tissue production (
Ching‐Yun Chen, Jia‐Ci Jhang, Chun‐Yi Peng, Quốc Cường Nguyễn, Feng‐Huei Lin, Hung‐Ming Wu, Shankung Lin   +6 more
wiley   +1 more source

New Manual Quantitative Polymerase Chain Reaction Assay Validated on Tongue Swabs Collected and Processed in Uganda Shows Sensitivity That Rivals Sputum-based Molecular Tuberculosis Diagnostics [PDF]

Amy Steadman, Alfred Andama, Alexey Ball, Job Mukwatamundu, Khushboo Khimani, Tessa Mochizuki, Lucy Asege, Alice Bukirwa, J Kato, David Katumba, Esther Kisakye, Wilson Mangeni, Sandra Mwebe, Martha Nakaye, Irene Nassuna, Justine Nyawere, Annet Nakaweesa, Catherine Cook, Patrick Phillips, Talemwa Nalugwa, Christine Bachman, Fred C. Semitala, Bernhard H. Weigl, John Connelly, William Worodria, Adithya Cattamanchi   +25 more
openalex   +1 more source

A novel mechanism of enhanced transcription activity and fidelity for influenza A viral RNA-dependent RNA polymerase [PDF]

, 2021
Xinzhou Xu, Lu Zhang, Julie Tung Sem Chu, Yuqing Wang, Alex W. H. Chin, Tin Hang Chong, Zixi Dai, Leo L. M. Poon, Peter Pak‐Hang Cheung, Xuhui Huang   +9 more
openalex   +1 more source

Anticancer sensitivities and biological characteristics of HCT116 cells resistant to the selective poly(ADP‐ribose) glycohydrolase inhibitor

FEBS Open Bio, EarlyView.
We analyzed alterations of PAR metabolism‐related proteins in PARG inhibitor‐resistant HCT116RPDD cells. Although PARG levels remained unchanged, HCT116RPDD cells exhibited reduced PARP1 and ARH3 expression and elevated PAR levels. Interestingly, HCT116RPDD cells exhibited slightly elevated intracellular NAD+/NADH and ATP levels. Our findings suggest a
Kaede Tsuda, Yoko Ogino, Akira Sato
wiley   +1 more source

Fidelity of base-pair recognition by a 3′–5′ polymerase: mechanism of the Saccharomyces cerevisiae tRNA^His guanylyltransferase [PDF]

, 2021
Krishna Patel, Paul Yourik, Jane E. Jackman   +2 more
openalex   +1 more source