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Current Protocols in Molecular Biology, 2008 AbstractThis unit describes DNA‐dependent, RNA‐dependent, and template‐independent RNA polymerases. DNA‐dependent RNA polymerases include the related bacteriophage T7, T3, and SP6 polymerases, the most commonly used RNA polymerases for in vitro transcription reactions. Reaction conditions to produce preparative quantities of transcribed RNA and labeled
Christopher J. Noren +3 more
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A mutant T7 RNA polymerase as a DNA polymerase.
The EMBO Journal, 1995We have identified a T7 RNA polymerase (RNAP) mutant that efficiently utilizes deoxyribonucleoside triphosphates. In vitro this mutant will synthesize RNA, DNA or 'transcripts' of mixed dNMP/rNMP composition depending on the mix of NTPs present in the synthesis reaction.
Rui J Sousa, Robert Padilla
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Annual Review of Biochemistry, 2002
▪ Abstract Any living cell is faced with the fundamental task of keeping the genome intact in order to develop in an organized manner, to function in a complex environment, to divide at the right time, and to die when it is appropriate. To achieve this goal, an efficient machinery is required to maintain the genetic information encoded in DNA during ...
Hubscher U, Maga G, Spadari S
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▪ Abstract Any living cell is faced with the fundamental task of keeping the genome intact in order to develop in an organized manner, to function in a complex environment, to divide at the right time, and to die when it is appropriate. To achieve this goal, an efficient machinery is required to maintain the genetic information encoded in DNA during ...
Hubscher U, Maga G, Spadari S
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[10] Purification of mammalian polymerases: DNA polymerase ε
1995Publisher Summary This chapter discusses the purification of mammalian polymerases, DNA polymerase ɛ. Pole ɛ is highly processive in the absence of proliferating cell nuclear antigen (PCNA) and antibody raised against Pol δ does not cross-react with Pol ɛ.
Stuart Linn, Gloria S.J. Chui
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T7 RNA polymerase: promoter structure and polymerase binding
Biochemistry, 1979The sequences of two promoters recognized by the phage-specificied T7 RNA polymerase are presented. The two are identical in sequence but for one base pair from the initiation point (as determined by the 5' sequence of the transcripts), denoted +1, to position -15. The common : formula: (see text), sequence also includes a region of hyphenated twofold
Joseph E. Coleman +3 more
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Polymerase Resistance to Polymerase Chain Reaction Inhibitors in Bone*
Journal of Forensic Sciences, 2009Abstract: Amplification of DNA from aged or degraded skeletal remains can be a challenging task, in part due to naturally occurring inhibitors of the polymerase chain reaction. PCR inhibitors may act by inactivating a polymerase itself, or compete with or bind other reaction components, although various polymerases may be differentially susceptible to
David R. Foran, Kenneth D. Eilert
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Structure of Taq polymerase with DNA at the polymerase active site
Nature, 1996The DNA polymerase from Thermus aquaticus (Taq polymerase) is homologous to Escherichia coli DNA polymerase I (Pol I) and likewise has domains responsible for DNA polymerase and 5' nuclease activities. The structures to the polymerase domains of Taq polymerase and of the Klenow fragment (KF) of Pol I are almost identical, whereas the structure of a ...
Jimin Wang +3 more
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2004
At the end of the 1950s Hoagland and Zamecnik had succeeded in obtaining an in vitro protein synthesis from ribosomes and crude acellular extracts. Ribosomes cannot by themselves achieve a specific protein synthesis and require an information originating in last analysis in the genes.
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At the end of the 1950s Hoagland and Zamecnik had succeeded in obtaining an in vitro protein synthesis from ribosomes and crude acellular extracts. Ribosomes cannot by themselves achieve a specific protein synthesis and require an information originating in last analysis in the genes.
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DNA Polymerase II, the Epsilon Polymerase of Saccharomyces cerevisiae
1993Publisher Summary This chapter discusses the data on DNA polymerase II in the context of current knowledge of eukaryotic DNA polymerases, DNA replication and its fidelity, and DNA repair. DNA polymerase II has been proffered as the “repair polymerase.” Contrary to the implicit assumption, DNA repair is not a single process but an array of different ...
Alan Morrison, Akio Sugino
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Polymerase Dependence of Autosticky Polymerase Chain Reaction
Analytical Biochemistry, 2000Róbert Schnell +2 more
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