Transitioning to DNA genomes in an RNA world
eLife, 2017 The unexpected ability of an RNA polymerase ribozyme to copy RNA into DNA has ramifications for understanding how DNA genomes evolved.
Razvan Cojocaru, Peter J Unrau
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Translesion synthesis in mammalian cells [PDF]
, 2006 DNA damage blocks the progression of the replication fork. In order to circumvent the damaged bases, cells employ specialized low stringency DNA polymerases, which are able to carry out translesion synthesis (TLS) past different types of damage. The five
Alan R. Lehmann +35 more
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Directed evolution of novel polymerase activities: Mutation of a DNA polymerase into an efficient RNA polymerase [PDF]
Proceedings of the National Academy of Sciences, 2002 The creation of novel enzymatic function is of great interest, but remains a challenge because of the large sequence space of proteins. We have developed an activity-based selection method to evolve DNA polymerases with RNA polymerase activity.
Gang, Xia +5 more
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Modified Nucleic Acids: Expanding the Capabilities of Functional Oligonucleotides
Molecules, 2020 In the last three decades, oligonucleotides have been extensively investigated as probes, molecular ligands and even catalysts within therapeutic and diagnostic applications.
Steven Ochoa, Valeria T. Milam
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Proficient replication of the yeast genome by a viral DNA polymerase [PDF]
, 2016 DNA replication in eukaryotic cells requires minimally three B-family DNA polymerases: Pol α, Pol δ, and Pol ϵ. Pol δ replicates and matures Okazaki fragments on the lagging strand of the replication fork.
Burgers, Peter M +2 more
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Norovirus Proteinase-Polymerase and Polymerase Are Both Active Forms of RNA-Dependent RNA Polymerase [PDF]
Journal of Virology, 2005 ABSTRACT In vitro mapping studies of the MD145 norovirus ( Caliciviridae ) ORF1 polyprotein identified two stable cleavage products containing the viral RNA-dependent RNA polymerase (RdRp) domains: ProPol (a precursor comprised of both the proteinase and polymerase) and Pol (the mature polymerase).
Gaël, Belliot +7 more
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Viruses, 2020 Current influenza vaccines have modest efficacy. This is especially true for current live attenuated influenza vaccines (LAIV), which have been inferior to the inactivated versions in recent years.
Andrew Cox +7 more
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P53 tumour-suppressor gene mutations are mainly localised on exon 7 in human primary and metastatic prostate cancer. [PDF]
, 1996 Mutations in the p53 tumour-suppressor gene are among the most common genetic alterations in human cancers. In the present study we analysed the mutations in the p53 tumor-suppressor gene in 25 primary and 20 metastatic human prostate cancer specimens ...
Chen, KM +5 more
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Accessibility of DNA in Chromatin to DNA Polymerase and RNA Polymerase [PDF]
Proceedings of the National Academy of Sciences, 1973 The accessibility of DNA in chromatin to both exogenous DNA polymerase and RNA polymerase is slight when compared to isolated DNA. DNA in extracted chromatin is somewhat more accessible to these enzymes than is DNA in the chromatin of isolated nuclei; and the DNA template of chromatin is more accessible to DNA polymerase than to RNA polymerase.
B, Silverman, A E, Mirsky
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Live culture-based qPCR screening of Taq DNA polymerase variants for resistance to PCR inhibitors
Frontiers in Bioengineering and BiotechnologyWe present a live culture PCR (LC-PCR) workflow that enables direct screening of randomly mutagenized Thermus aquaticus (Taq) and Klentaq1 DNA polymerase libraries without enzyme purification.
Milko B. Kermekchiev +3 more
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