Results 41 to 50 of about 2,178,231 (341)

Versatile SARS-CoV-2 Reverse-Genetics Systems for the Study of Antiviral Resistance and Replication

Viruses, 2022
The COVID-19 pandemic continues to threaten healthcare systems worldwide due to the limited access to vaccines, suboptimal treatment options, and the continuous emergence of new and more transmissible SARS-CoV-2 variants.
Ulrik Fahnøe, Long V. Pham, Carlota Fernandez-Antunez, Rui Costa, Lizandro René Rivera-Rangel, Andrea Galli, Shan Feng, Lotte S. Mikkelsen, Judith M. Gottwein, Troels K. H. Scheel, Santseharay Ramirez, Jens Bukh   +11 more
doaj   +1 more source

alpha-helix E of Spo0A is required for sigma(A)- but not for sigma(H)-dependent promoter activation in Bacillus subtilis [PDF]

, 2004
At the onset of endospore formation in Bacillus subtilis, the DNA binding protein Spo0A activates transcription from two types of promoters. The first type includes the spoIIG and spoIIE promoters, which are used by sigma(A)-RNA polymerase, whereas the ...
Brannigan, J.A., Kumar, A., Moran, C.P.
core   +2 more sources

Plant or fungal sequences? An alternative optimized PCR protocol to avoid ITS (nrDNA) misamplification

Brazilian Archives of Biology and Technology, 2010
The nuclear ribosomal DNA internal transcribed spacers (ITS1 and ITS2) from leaves of Drosera (Droseraceae) were amplified using "universal" primers.
Vitor Fernandes Oliveira de Miranda, Vanderlei Geraldo Martins, Antonio Furlan, Maurício Bacci Jr.   +3 more
doaj   +1 more source

Preparation and functional evaluation of monoclonal antibodies targeting Hepatitis B Virus Polymerase

Virulence, 2021
HBV pol plays a critical role in the replication of hepatitis B virus (HBV). Previous studies conducted on HBV pol have produced limited evidence on HBV pol expression due to the lack of effective detection methods.
Song Hu, Hualong Xiong, Xiaozhen Kang, Shaojuan Wang, Tianying Zhang, Quan Yuan, Deying Tian   +6 more
doaj   +1 more source

Transcription-associated breaks in Xeroderma Pigmentosum group D cells from patients with combined features of Xeroderma Pigmentosum and Cockayne Syndrome [PDF]

, 2005
Defects in the XPD gene can result in several clinical phenotypes, including xeroderma pigmentosum (XP), trichothiodystrophy, and, less frequently, the combined phenotype of XP and Cockayne syndrome (XP-D/CS).
Andressoo, Jaan-Olle, Botta, Elena, Fousteri, Maria I., Fujimoto, Mitsuo, Harries, Lorna W., Jaspers, Nicolaas G. J., Lehmann, Alan R., McDaniel, Lisa D., Mitchell, Jay, Mullenders, Leon H., Stefanini, Miria, Theron, Therina, Volker, Marcel   +12 more
core   +3 more sources

Identification and Diagnosis of Cutaneous Leishmaninsis by Polymerase Chain Reaction Assay (Pcr) in Najaf Alashraf Province

Kufa Journal for Nursing Sciences, 2013
Objective: The present study aimed to determine the effectiveness of a polymerase chain reaction (PCR) technique for identification and differentiation of the cutaneous leishmaniasis parasite in clinical sample collected of lesion exudates patients ...
Haitham Mohammed Alawadi, Ban Shakir Alshukur   +1 more
doaj   +1 more source

Spt5 Cooperates with Human Immunodeficiency Virus Type 1 Tat by Preventing Premature RNA Release at Terminator Sequences [PDF]

, 2002
The human immunodeficiency virus type 1 (HIV-1) Tat protein activates transcription elongation by stimulating the Tat-activated kinase (TAK/p-TEFb), a protein kinase composed of CDK9 and its cyclin partner, cyclin T1.
Bourgeois, Cyril F, Churcher, Mark J, Karn, Jonathan, Kim, Young Kyeung, West, Michelle J   +4 more
core   +3 more sources

ANP32B, or not to be, that is the question for influenza virus

eLife, 2019
The evolutionary history of ANP32 proteins impacts how influenza virus jumps from birds to mammals.
Steven F Baker, Andrew Mehle
doaj   +1 more source

Plasmodium falciparum gametogenesis essential protein 1 (GEP1) is a transmission‐blocking target

FEBS Letters, EarlyView.
This study shows Plasmodium falciparum GEP1 is vital for activating sexual stages of malarial parasites even independently of a mosquito factor. Knockout parasites completely fail gamete formation even when a phosphodiesterase inhibitor is added. Two single‐nucleotide polymorphisms (V241L and S263P) are found in 12%–20% of field samples.
Frederik Huppertz, Milagros Siebeck Caturelli, Lina S. Lehmann, Florian Kurth, Alexander G. Maier, Kai Matuschewski   +5 more
wiley   +1 more source

Viral polymerase-host interaction analysis reveals that the association between avian NUP93 and PB1 promotes H5N6 avian influenza virus replication

Microbiology Spectrum
The H5N6 avian influenza virus, a highly pathogenic strain, poses a significant threat to poultry production and public health. The RNA-dependent RNA polymerase (comprising PB1, PB2, and PA proteins) and nucleoprotein of highly pathogenic avian influenza
Lei Cao, Ting Wang, Peilei Ren, Chuxing Cheng, Haiying Mao, Xianfeng Hui, Xian Lin, Xiaotong Hu, Xiaomei Sun, Kun Huang, Meilin Jin   +10 more
doaj   +1 more source