Results 41 to 50 of about 2,105,688 (341)
Plasmid-based lacZalpha assay for DNA polymerase fidelity: application to archaeal family-B DNA polymerase [PDF]
, 2009 The preparation of a gapped pUC18 derivative, containing the lacZalpha reporter gene in the single-stranded region, is described. Gapping is achieved by flanking the lacZalpha gene with sites for two related nicking endonucleases, enabling the excision ...
Bebenek +28 more
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Polymerase Chain Reaction [PDF]
Journal of Investigative Dermatology, 2013 The advent of the polymerase chain reaction (PCR) radically transformed biological science from the time it was first discovered (Mullis, 1990). For the first time, it allowed for specific detection and production of large amounts of DNA. PCR-based strategies have propelled huge scientific endeavors such as the Human Genome Project.
Nidhi Avashia, Lilit Garibyan
openaire +2 more sources
DNA polymerases required for repair of UV-induced damage in Saccharomyces cerevisiae [PDF]
, 1995 The ability of yeast DNA polymerase mutant strains to carry out repair synthesis after UV irradiation was studied by analysis of postirradiation molecular weight changes in cellular DNA.
Budd, Martin E., Campbell, Judith L.
core +2 more sources
Viruses, 2023 The human respiratory syncytial virus (RSV) is a negative-sense, single-stranded RNA virus. It is the major cause of severe acute lower respiratory tract infection in infants, the elderly population, and immunocompromised individuals.
Priscila Sutto-Ortiz +3 more
doaj +1 more source
Viruses, 2020 The Eurasian avian-like swine (EA) H1N1 virus has affected the Chinese swine industry, and human infection cases have been reported occasionally. However, little is known about the pathogenic mechanism of EA H1N1 virus.
Mengkai Cai +10 more
doaj +1 more source
Cell, 1999 The appearance of these structures represents a historic advance in our understanding of one of molecular biology's most remarkable enzymes. Here we have suggested several possible structural bases for key steps in its function. Although speculative, they should serve as stimuli for future work.
Robert Landick, Rachel A. Mooney
openaire +3 more sources
Viruses, 2023 Background: As long as COVID-19 endures, viral surface proteins will keep changing and new viral strains will emerge, rendering prior vaccines and treatments decreasingly effective.
Brittany A. Comunale +5 more
doaj +1 more source
International Journal of Neonatal Screening, 2021 A variant in the POLG gene is the leading cause of a heterogeneous group of mitochondrial disorders. No definitive treatment is currently available. Prenatal and newborn screening have the potential to improve clinical outcome of patients affected with ...
Kanokwan Sriwattanapong +5 more
doaj +1 more source
Biophysical Chemistry, 2011 Molecular motors can exhibit Brownian ratchet or power stroke mechanisms. These mechanistic categories are related to transition state position: An early transition state suggests that chemical energy is stored and then released during the step (stroke) while a late transition state suggests that the release of chemical energy rectifies thermally ...
Eric A. Galburt +2 more
openaire +4 more sources
Molecular dissection of the domain architecture and catalytic activities of human PrimPol [PDF]
, 2014 PrimPol is a primase–polymerase involved in nuclear and mitochondrial DNA replication in eukaryotic cells. Although PrimPol is predicted to possess an archaeo-eukaryotic primase and a UL52-like zinc finger domain, the role of these domains has not been ...
Aidan J. Doherty +45 more
core +2 more sources