Results 41 to 50 of about 798,318 (192)

Ion channel function of polycystin‐2/polycystin‐1 heteromer revealed by structure‐guided mutagenesis

FEBS Letters, EarlyView.
Mutations in polycystin‐1 (PC1) or polycystin‐2 (PC2) cause autosomal‐dominant polycystic kidney disease (ADPKD). We generated a novel gain‐of‐function PC2/PC1 heteromeric ion channel by mutating pore‐blocking residues. Moreover, we demonstrated that PC2 will preferentially assemble with PC1 to form heteromeric complexes when PC1 is co‐expressed ...
Tobias Staudner, Juthamas Khamseekaew, M. Gregor Madej, Linda Geiges, Bardha Azemi, Christine Ziegler, Christoph Korbmacher, Alexandr V. Ilyaskin   +7 more
wiley   +1 more source

Accessibility of DNA in Chromatin to DNA Polymerase and RNA Polymerase [PDF]

Proceedings of the National Academy of Sciences, 1973
The accessibility of DNA in chromatin to both exogenous DNA polymerase and RNA polymerase is slight when compared to isolated DNA. DNA in extracted chromatin is somewhat more accessible to these enzymes than is DNA in the chromatin of isolated nuclei; and the DNA template of chromatin is more accessible to DNA polymerase than to RNA polymerase.
Bert Silverman, Alfred E. Mirsky
openaire   +3 more sources

A stepwise emergence of evolution in the RNA world

FEBS Letters, EarlyView.
How did biological evolution emerge from chemical reactions? This perspective proposes a gradual scenario of self‐organization among RNA molecules, where catalytic feedback on random mixtures plays the central role. Short oligomers cross‐ligate, and self‐assembly enables heritable variations. An event of template‐externalization marks the transition to
Philippe Nghe
wiley   +1 more source

Analysis of the Essential Functions of the C-terminal Protein/Protein Interaction Domain of Saccharomyces cerevisiae pol epsilon and Its Unexpected Ability to Support Growth in the Absence of the DNA Polymerase Domain [PDF]

, 1999
As first observed by Wittenberg (Kesti, T., Flick, K., Keranen, S., Syvaoja, J. E., and Wittenburg, C. (1999) Mol. Cell 3, 679-685), we find that deletion mutants lacking the entire N-terminal DNA polymerase domain of yeast pol epsilon are viable ...
Campbell, Judith L., Dua, Rajiv, Levy, Daniel L.   +2 more
core  

Structure and mechanism of human DNA polymerase η [PDF]

, 2010
The variant form of the human syndrome xeroderma pigmentosum (XPV) is caused by a deficiency in DNA polymerase eta (Pol eta), a DNA polymerase that enables replication through ultraviolet-induced pyrimidine dimers.
A Alt, A Mees, A Nicholls, A Scrima, AJ McCoy, AJ McCoy, Alan R. Lehmann, AR Lehmann, AT Brünger, BC Broughton, C Masutani, C Masutani, C Vonrhein, Chikahide Masutani, Christian Biertümpfel, DE Brash, DF Jarosz, DG Vassylyev, DV Bugreev, E Bassett, E Glick, F Wang, Fumio Hanaoka, H Inui, H Ling, H Ling, H Ling, H Park, H Saribasak, IW Davis, J Bauer, J Di Lucca, J Trincao, J Yao, Jae Young Lee, JH Min, K Sugasawa, L Jia, L Rey, L Wang, M Tanioka, Mark Gregory, MJ McIlwraith, P Emsley, R Kusumoto, R Kusumoto, RC Wilson, RE Johnson, RJ Kokoska, S Broyde, S Creighton, S Lone, Santiago Ramón-Maiques, SD McCulloch, SG Chaney, T Hishida, T Kawamoto, TC Terwilliger, W Kabsch, W Yang, W Yang, WA Hendrickson, Wei Yang, Y Li, Ye Zhao, Yuji Kondo, Z Otwinowski   +66 more
core   +2 more sources

Modifications in FLAP's second cytosolic loop influence 5‐LOX interaction, inhibitor binding, and leukotriene formation

FEBS Letters, EarlyView.
The enzyme 5‐lipoxygenase (5‐LOX) catalyzes the first step in the biosynthesis of leukotrienes (LTs) involved in inflammatory pathophysiology. After cellular stimulation, 5‐LOX translocates to the nucleus, interacting with the 5‐LOX‐activating protein (FLAP) to form LTA4 from arachidonic acid (AA).
Erik Romp, Katharina Rataj, Stefanie König, Marcia E. Newcomer, Oliver Werz, Ulrike Garscha   +5 more
wiley   +1 more source

Brucella NyxA and NyxB dimerization enhances effector function during infection

FEBS Letters, EarlyView.
Brucella abortus thrives inside cells thanks to the translocation of effector proteins that fine‐tune cellular functions. NyxA and NyxB are two effectors that destabilize the nucleolar localization of their host target, SENP3. We show that the Nyx proteins directly interact with each other and that their dimerization is essential for their function ...
Lison Cancade‐Veyre, Arthur Louche, Francine C. A. Gérard, Laurent Terradot, Suzana P. Salcedo   +4 more
wiley   +1 more source

Translesion synthesis in mammalian cells [PDF]

, 2006
DNA damage blocks the progression of the replication fork. In order to circumvent the damaged bases, cells employ specialized low stringency DNA polymerases, which are able to carry out translesion synthesis (TLS) past different types of damage. The five
Alan R. Lehmann, Avkin, Avkin, Bienko, Garg, Gibbs, Gibbs, Gibbs, Guo, Haracska, Hoege, Huang, Johnson, Kannouche, Kannouche, King, Lehmann, Lemontt, Ling, Maher, Masutani, Masutani, McCulloch, Murakumo, Nair, Nakajima, Nelson, Nelson, Nelson, Ogi, Ohmori, Pages, Stelter, Tissier, Vidal, Watanabe   +35 more
core   +2 more sources

The carboxylate “gripper” of the substrate is critical for C‐4 stereo‐inversion by UDP‐glucuronic acid 4‐epimerase

FEBS Letters, EarlyView.
UDP‐glucuronic acid 4‐epimerase (UGAepi) catalyzes NAD+‐dependent interconversion of UDP‐glucuronic acid (UDP‐GlcA) and UDP‐galacturonic acid (UDP‐GalA) via C4‐oxidation, 4‐keto‐intermediate rotation, and C4‐reduction. Here, Borg et al. examined the role of the substrate's carboxylate group in the enzymic mechanism by analyzing NADH‐dependent reduction
Annika J. E. Borg, Laura De Cnop, Bernd Nidetzky   +2 more
wiley   +1 more source

A model for transition of 5 '-nuclease domain of DNA polymerase I from inert to active modes [PDF]

, 2011
Bacteria contain DNA polymerase I (PolI), a single polypeptide chain consisting of similar to 930 residues, possessing DNA-dependent DNA polymerase, 3'-5' proofreading and 5'-3' exonuclease (also known as flap endonuclease) activities.
A Díaz, B Shen, C Orlando, CJ Bornarth, CM Joyce, CW Gardiner, DL Ho, E Delagoutte, H Risken, IV Kutyavin, Janine Santos, JJ Harrington, Jon R. Sayers, JR Sayers, JR Sayers, JR Sayers, K Datta, K Makiela-Dzbenska, KL Nakamaye, L Zheng, L Zietlow, LM Allen, M Imai, MR Lieber, MRG Hodskinson, P Robins, P Setlow, P Xie, P Xie, P Xie, PD Gutman, PG de Gennes, PH Patel, Ping Xie, PM Holland, PM Kampmeyer, RC Lundquist, RL Honeycutt, RM Turner, S Fukushima, SJ Garforth, TA Ceska, UK Urs, V Lyamichev, V Lyamichev, WS Kelley, Y Kim, Y Kurosawa, Y Nagata, Y Xu, Z Bu   +50 more
core   +4 more sources