Results 61 to 70 of about 1,270,868 (225)
Electrostatic map of T7 DNA. Comparative analysis of functional and electrostatic properties of T7 RNA polymerase specific promoters [PDF]
, 2013 The entire T7 bacteriophage genome contains 39937 base pairs (Database NCBI RefSeq N1001604). Here, electrostatic potential distribution around double helical T7 DNA was calculated by Coulomb method using the computer program of Sorokin A.A. Electrostatic profiles of 17 promoters recognized by T7 phage specific RNA polymerase were analyzed.
arxiv +1 more source
Accessibility of DNA in Chromatin to DNA Polymerase and RNA Polymerase [PDF]
Proceedings of the National Academy of Sciences, 1973 The accessibility of DNA in chromatin to both exogenous DNA polymerase and RNA polymerase is slight when compared to isolated DNA. DNA in extracted chromatin is somewhat more accessible to these enzymes than is DNA in the chromatin of isolated nuclei; and the DNA template of chromatin is more accessible to DNA polymerase than to RNA polymerase.
Bert Silverman, Alfred E. Mirsky
openaire +3 more sources
A new contribution to Megasporoporia sensu lato: Six new species and three new combinations
Frontiers in Microbiology, 2022 Megasporoporia sensu lato has recently been intensively studied in China and South America, and four independent clades representing four genera have been recognized phylogenetically. In this study, more samples, mostly from subtropical and tropical Asia,
Ya-Rong Wang +7 more
doaj +1 more source
Mechanical Properties of Transcription [PDF]
Phys. Rev. Lett. 118, 268101 (2017), 2016 Recently the physical characterization of a number of biological processes has proven indispensable for a full understanding of natural phenomena. One such example is the mechanical properties of transcription, which have been shown to have significant effects in gene expression.
arxiv +1 more source
RNA amplification for successful gene profiling analysis
Journal of Translational Medicine, 2005 The study of clinical samples is often limited by the amount of material available to study. While proteins cannot be multiplied in their natural form, DNA and RNA can be amplified from small specimens and used for high-throughput analyses.
Wang Ena
doaj +1 more source
Replicative DNA Polymerases [PDF]
Cold Spring Harbor Perspectives in Biology, 2013 In 1959, Arthur Kornberg was awarded the Nobel Prize for his work on the principles by which DNA is duplicated by DNA polymerases. Since then, it has been confirmed in all branches of life that replicative DNA polymerases require a single-stranded template to build a complementary strand, but they cannot start a new DNA strand de novo.
Johansson, Erik, Dixon, Nicholas
openaire +4 more sources
Viruses, 2019 The L-protein of vesicular stomatitis virus (VSV) is a single-chain multi-domain RNA-dependent RNA polymerase. Previously reported attempts of intramolecular insertions of fluorescent proteins into the L-protein resulted in temperature-sensitive and ...
Emmanuel Heilmann +7 more
doaj +1 more source
Polyprotein Processing as a Determinant for in Vitro Activity of Semliki Forest Virus Replicase
Viruses, 2017 Semliki Forest virus (SFV) is an arthropod-borne alphavirus that induces membrane invaginations (spherules) in host cells. These harbor the viral replication complexes (RC) that synthesize viral RNA.
Maija K. Pietilä +3 more
doaj +1 more source
A design proposal of the reverse DNA polymerase (3'-5' direction) [PDF]
arXiv, 2013 DNA Polymerase is an enzyme that participates in the process of DNA replication. In general, forward DNA polymerase moves along the template strand in a 3'-5' direction, and the daughter strand is formed in a 5'-3' direction. This paper theoretically analyzes the mechanism of forward DNA polymerase, and gives the design proposal of a new reverse DNA ...
arxiv
DNA Polymerase Profiling [PDF]
, 2008 We report a simple homogeneous fluorescence assay for quantification of DNA polymerase function in high throughput. The fluorescence signal is generated by the DNA polymerase triggering opening of a molecular beacon extension of the template strand. A resulting distance alteration is reported by fluorescence resonance energy transfer between two dyes ...
openaire +3 more sources