Results 121 to 130 of about 1,452,270 (349)

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

open access: yesFEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon   +6 more
wiley   +1 more source

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

open access: yesFEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li   +6 more
wiley   +1 more source

High throughput oscillating-flow structure for polymerase chain reaction

open access: yesAIP Advances
Polymerase chain reaction (PCR) is a revolutionary biomolecule technology that drives the development of bioscience. The current work reports a high throughput oscillating-flow PCR structure.
Ranran Zhou   +7 more
doaj   +1 more source

Identification of Campylobacter Jejuni Using Polymerase Chain Reaction Method [PDF]

open access: yes, 2013
Campylobacter jejuni is a curved-rod shaped bacteria, non-spore, microaerophilic Gram-negative and motile. It is commonly found in the feces of animals.
Angeliya, L. (Liza)   +1 more
core  

Confidence intervals for nonhomogeneous branching processes and polymerase chain reactions

open access: yes, 2005
We extend in two directions our previous results about the sampling and the empirical measures of immortal branching Markov processes. Direct applications to molecular biology are rigorous estimates of the mutation rates of polymerase chain reactions ...
Piau, Didier
core   +4 more sources

Systemic T Cell Receptor Profiling Reveals Adaptive Immune Activation and Potential Immune Signatures of Diagnosis and Brain Atrophy in Epilepsy

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective Epilepsy is increasingly associated with immune dysregulation and inflammation. The T cell receptor (TCR), a key mediator of adaptive immunity, shows repertoire alterations in various immune‐mediated diseases. The unique TCR sequence serves as a molecular barcode for T cells, and clonal expansion accompanied by reduced overall TCR ...
Yong‐Won Shin   +12 more
wiley   +1 more source

Real time polymerase chain reaction for the quantitative detection [PDF]

open access: yes, 2009
Soybean protein is reported to be the most widely used vegetable protein in the meat industry. Several characteristics of soybean protein such as the emulsifier properties, preventing the coalescence of fat during heating, and the increased capacity of ...
Amaral, Joana S.   +3 more
core  

CSF Levels of NPTX2 Are Associated With Less Brain Atrophy Over Time in Cognitively Unimpaired Individuals

open access: yesAnnals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Introduction Neuronal pentraxin 2 (NPTX2) is a synaptic protein involved in synaptic plasticity and regulation of neuronal excitability. Lower baseline cerebrospinal fluid (CSF) NPTX2 levels have been shown to be associated with an earlier onset of mild cognitive impairment (MCI), a pre‐dementia syndrome, even after CSF Alzheimer's Disease (AD)
Juan P. Vazquez   +12 more
wiley   +1 more source

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