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Inverse Polymerase Chain Reaction
Nature Biotechnology, 1989We describe a procedure that extends the utility of the polymerase chain reaction (PCR) in allowing the geometric amplification of an unknown DNA sequence that flanks a core region of known sequence. DNA containing the core region is digested with appropriate restriction enzymes to produce a fragment of suitable size for PCR amplification.
James W. Ajioka +3 more
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ChemInform Abstract: Polymerase Chain Reaction
ChemInform, 1990AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Corey H. Levenson, Norman Arnheim
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1990
The development of a new technology can lead to novel approaches to answering current scientific questions. A new technology can also often lead to the framing of scientific questions that previously were considered intractable and not amenable to experimental investigation.
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The development of a new technology can lead to novel approaches to answering current scientific questions. A new technology can also often lead to the framing of scientific questions that previously were considered intractable and not amenable to experimental investigation.
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2001
The polymerase chain reaction (PCR) was introduced in 1985 by Saiki et al. (1), who recognized that sequence-specific oligonucleotides could be used to prime synthesis of complementary DNA strands by any of several DNA polymerases and that these primers could be chosen so as to replicate both strands of the DNA positioned between their cognate ...
Philip N. Howles, Yuri E. Nikiforov
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The polymerase chain reaction (PCR) was introduced in 1985 by Saiki et al. (1), who recognized that sequence-specific oligonucleotides could be used to prime synthesis of complementary DNA strands by any of several DNA polymerases and that these primers could be chosen so as to replicate both strands of the DNA positioned between their cognate ...
Philip N. Howles, Yuri E. Nikiforov
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Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction [PDF]
, 2009 Polymerase chain reaction (PCR) enables one to determine if a specific needle is present in a haystack, and it can be used as a step toward the characterization of the needle. It is a quick, powerful, inexpensive DNA amplification technique that has become a fundamental tool in molecular pathology.
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1999
The polymerase chain reaction (PCR) (1) is a rapid technique for in vitro amplification of a specific DNA fragment by use of two short single- stranded primers flanking this fragment. Through repeated cycles of heat denaturation of the double-stranded DNA template, primer annealing, and primer extension using a heat-stable polymerase, the fragment of ...
Iva Singh-Sawhney, Friedhelm Hildebrandt
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The polymerase chain reaction (PCR) (1) is a rapid technique for in vitro amplification of a specific DNA fragment by use of two short single- stranded primers flanking this fragment. Through repeated cycles of heat denaturation of the double-stranded DNA template, primer annealing, and primer extension using a heat-stable polymerase, the fragment of ...
Iva Singh-Sawhney, Friedhelm Hildebrandt
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Immunocapture-Polymerase Chain Reaction
2005Antibody capture of viruses can be used as a preparatory step in nucleic acid amplification techniques. Immunocapture of virus particles can be used to streamline and/or optimize the concentration, purification and specificity requirements of polymerase chain reaction assays.
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1994
Edited by the inventor of polymerase chain reaction (PCR) and the 1993 Nobel Prize winner in Chemistry, Kary Mullis, as well as two experts in the field, this handbook provides up-to-date methodological protocols from the world's leading laboratories, in addition to new techniques and enhanced applications not yet available in book form.
Richard A. Gibbs +2 more
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Edited by the inventor of polymerase chain reaction (PCR) and the 1993 Nobel Prize winner in Chemistry, Kary Mullis, as well as two experts in the field, this handbook provides up-to-date methodological protocols from the world's leading laboratories, in addition to new techniques and enhanced applications not yet available in book form.
Richard A. Gibbs +2 more
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1998
The polymerase chain reaction (PCR) has been developed to detect very small quantities of nucleic acid by amplification of a segment of the DNA situated between two regions of known nucleotide sequence (Saiki et al. 1985). Such a segment is flanked by two oligonucleotides which serve as primers for a series of reactions that are catalysed by a DNA ...
Jeanne Dijkstra, Cees P. de Jager
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The polymerase chain reaction (PCR) has been developed to detect very small quantities of nucleic acid by amplification of a segment of the DNA situated between two regions of known nucleotide sequence (Saiki et al. 1985). Such a segment is flanked by two oligonucleotides which serve as primers for a series of reactions that are catalysed by a DNA ...
Jeanne Dijkstra, Cees P. de Jager
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