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Infection Control and Hospital Epidemiology, 1991
Polymerase chain reaction is a simple method of DNA amplification which permits a single short sequence to be replicated in the Laboratory many million times. The procedure takes a few hours at most, and can be adapted for non-radioactive analysis. As little as a single human cel!, or single virus, can be studied.
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Polymerase chain reaction is a simple method of DNA amplification which permits a single short sequence to be replicated in the Laboratory many million times. The procedure takes a few hours at most, and can be adapted for non-radioactive analysis. As little as a single human cel!, or single virus, can be studied.
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Immunocapture-Polymerase Chain Reaction
2005Antibody capture of viruses can be used as a preparatory step in nucleic acid amplification techniques. Immunocapture of virus particles can be used to streamline and/or optimize the concentration, purification and specificity requirements of polymerase chain reaction assays.
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1990
The development of a new technology can lead to novel approaches to answering current scientific questions. A new technology can also often lead to the framing of scientific questions that previously were considered intractable and not amenable to experimental investigation.
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The development of a new technology can lead to novel approaches to answering current scientific questions. A new technology can also often lead to the framing of scientific questions that previously were considered intractable and not amenable to experimental investigation.
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1999
The polymerase chain reaction (PCR) (1) is a rapid technique for in vitro amplification of a specific DNA fragment by use of two short single- stranded primers flanking this fragment. Through repeated cycles of heat denaturation of the double-stranded DNA template, primer annealing, and primer extension using a heat-stable polymerase, the fragment of ...
Friedhelm Hildebrandt, Iva Singh-Sawhney
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The polymerase chain reaction (PCR) (1) is a rapid technique for in vitro amplification of a specific DNA fragment by use of two short single- stranded primers flanking this fragment. Through repeated cycles of heat denaturation of the double-stranded DNA template, primer annealing, and primer extension using a heat-stable polymerase, the fragment of ...
Friedhelm Hildebrandt, Iva Singh-Sawhney
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Polymerase Chain Reaction and Reverse Transcription-Polymerase Chain Reaction
2009Polymerase chain reaction (PCR) enables one to determine if a specific needle is present in a haystack, and it can be used as a step toward the characterization of the needle. It is a quick, powerful, inexpensive DNA amplification technique that has become a fundamental tool in molecular pathology.
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1998
The development of the polymerase chain reaction (PCR) technique is a milestone in genome analysis. The basic concept was first tested with Klenow polymerase but the real breakthrough came when a thermostable DNA polymerase, Taq polymerase (1), was isolated and purified.
Rita Aert +4 more
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The development of the polymerase chain reaction (PCR) technique is a milestone in genome analysis. The basic concept was first tested with Klenow polymerase but the real breakthrough came when a thermostable DNA polymerase, Taq polymerase (1), was isolated and purified.
Rita Aert +4 more
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1995
Abstract The polymerase chain reaction (PCR) provides the means to amplify specific sequences of DNA an essentially unlimited number of times. The method was first used to amplify human DNA sequences (1-4) and since then has been used extensively as an alternative or an adjunct to cloning in bacterial vectors for analysis of eukaryotic ...
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Abstract The polymerase chain reaction (PCR) provides the means to amplify specific sequences of DNA an essentially unlimited number of times. The method was first used to amplify human DNA sequences (1-4) and since then has been used extensively as an alternative or an adjunct to cloning in bacterial vectors for analysis of eukaryotic ...
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The assembly, regulation and function of the mitochondrial respiratory chain
Nature Reviews Molecular Cell Biology, 2021Irene Vercellino, Leonid A Sazanov
exaly