Results 1 to 10 of about 3,760 (186)

An acidic patch in the unstructured N‐terminus modulates LSD1 activity

Protein Science, Volume 34, Issue 12, December 2025.
Abstract Lysine‐specific demethylase 1 (LSD1) plays a crucial role in chromatin organization and gene regulation by removing methyl groups from histone and non‐histone substrates. While its catalytic core is well characterized, the functional contributions of its intrinsically disordered N‐terminal region remain less understood.
Franziska Dukatz, Hermann Timofeev, Philipp Schnee, Philipp Rathert   +3 more
wiley   +1 more source

Mechanisms of DNA damage, repair, and mutagenesis [PDF]

, 2017
Living organisms are continuously exposed to a myriad of DNA damaging agents that can impact health and modulate disease-states. However, robust DNA repair and damage-bypass mechanisms faithfully protect the DNA by either removing or tolerating the ...
Chatterjee, Nimrat, Walker, Graham C
core   +1 more source

Optimizing (Bio) Catalysis with Liquid‐Liquid Phase Separation Systems

ChemSystemsChem, Volume 7, Issue 6, November 2025.
This review highlights how LLPS creates membraneless compartments involved in different scenarios in nature. It further explores different examples that focus on the development of synthetic coacervates that enhance catalytic efficiency by concentrating reactants, or by the direct influence of the coacervates building blocks themselves.
David Q. P. Reis, Joana Calvário, Inês Chibeles, Mohit Kumar, Ana S. Pina   +4 more
wiley   +1 more source

cDNAs encoding the large subunit of human replication factor C [PDF]

, 1993
Replication factor C (RFC) is a multisubunit, DNA polymerase accessory protein required for the coordinated synthesis of both DNA strands during simian virus 40 DNA replication in vitro. Previous studies have shown that RFC is a DNA-dependent ATPase that
Bunz, F., Kobayashi, R., Stillman, B.
core  

A CRISPRi Gene Regulation System for Bifidobacteria

Microbial Biotechnology, Volume 18, Issue 11, November 2025.
We report the development of a single plasmid‐based CRISPR interference system based on a catalytically dead Cas9 from Streptococcus thermophilus for targeted gene repression in bifidobacteria. This system is capable of repressing genes in a diverse range of bifidobacteria enabling functional genomics studies in non‐model strains.
Lisa Friess, Douwe van Sinderen, Ciaran Lee   +2 more
wiley   +1 more source

How cancer cells hijack DNA double-strand break repair pathways to gain genomic instability [PDF]

, 2015
DNA double-strand breaks (DSBs) are a significant threat to the viability of a normal cell, since they can result in loss of genetic material if mitosis or replication is attempted in their presence.
Arana, Audebert, Bartek, Bartkova, Barton, Bell, Bentley, Bentley, Beucher, Bolderson, Bouwman, Brady, Bryant, Budman, Budman, Bunting, Callen, Ceccaldi, Cerbinskaite, Chan, Chapman, Chapman, Choudhury, Cornforth, Deckbar, Deckbar, Deckbar, Dion, Dohrn, Ensminger, Erkko, Falck, Fan, Farmer, Fernandez-Capetillo, Fox, Fradet-Turcotte, Garcia, Gaymes, Gerhold, Geuting, Ghezraoui, Goodarzi, Helmink, Hu, Huertas, Jacot, Jasin, Jeggo, Jeggo, Jiang, Johnson, Kakarougkas, Kakarougkas, Kakarougkas, Kastan, Kemp, Kuhne, Li, Liang, Lieber, Lobrich, Loeb, Loucas, Maacke, Markus Löbrich, Mateos-Gomez, Michailidou, Muvarak, Neubauer, Panier, Panier, Penny A. Jeggo, Plisiecka-Halasa, Povirk, Prakash, Qiao, Radhakrishnan, Rahman, Riballo, Richardson, Roth, Sallmyr, Sarbajna, Seeber, Shanbhag, Shibata, Shibata, Shibata, Simsek, Soni, Steinel, Stephan, Syeda, Symington, Takenaka, Tepsuporn, Tobin, Tobin, Uziel, Wang, Wang, Ward, Waters, Waters, Williams, Woodbine, Woodbine, Yun, Zelensky, Zimmermann   +110 more
core   +1 more source

Wet Lab Protocols Matter: Choice of DNA Extraction and Library Preparation Protocols Bias Ancient Oral Microbiome Recovery

Molecular Ecology Resources, Volume 25, Issue 8, November 2025.
ABSTRACT Ancient DNA (aDNA) analysis of archaeological dental calculus has provided a wealth of insights into ancient health, demography and lifestyles. However, the workflow for ancient metagenomics is still evolving, raising concerns about reproducibility.
Sterling L. Wright, Muslih Abdul‐Aziz, Grace N. Blaha, Christine K. Ta, Abigail Gancz, Iyunoluwa J. Ademola‐Popoola, Anna Szécsényi‐Nagy, Paul C. Sereno, Laura S. Weyrich   +8 more
wiley   +1 more source

The bZIP3‐AS1 complex promotes CONSTANS‐induced FLOWERING LOCUS T activation in a daylength‐dependent manner

Journal of Integrative Plant Biology, Volume 67, Issue 11, Page 2982-2998, November 2025.
The Arabidopsis bZIP3 protein works in conjunction with ASYMMETRIC LEAVES1 to facilitate the function of the CONSTANS protein in activating FLOWERING LOCUS T expression at the end of the day in a day length‐dependent manner. ABSTRACT Plants monitor daylength to synchronize their flowering time with their surroundings and thus maximize reproductive ...
Wonbok Lee, Sun Ho Kim, Junsang Park, So Hee Yoon, Sung Won Cho, Nayoung Lee, Shogo Ito, Takato Imaizumi, Jong Chan Hong, Woo Sik Chung, Young Hun Song   +10 more
wiley   +1 more source

Mechanisms of base selection by the E.coli mispaired uracil glycosylase [PDF]

, 2008
The repair of the multitude of single-base lesions formed daily in the cells of all living organisms is accomplished primarily by the base-excision repair (BER) pathway that initiates repair through a series of lesion-selective glycosylases.
Darwanto, Agus, Goddard, William, III, Liu, Pingfang, Pascal, Tod, Sowers, Lawrence C., Theruvathu, Jacob A., Valinluck Lao, Victoria   +6 more
core   +2 more sources

Efficient and specific gene knockdown by small interfering RNAs produced in bacteria [PDF]

, 2014
Synthetic small interfering RNAs (siRNAs) are an indispensable tool to investigate gene function in eukaryotic cells1,2 and may be used for therapeutic purposes to knockdown genes implicated in disease3. Thus far, most synthetic siRNAs have been produced
Deighan, Padraig, Huang, Linfeng, Jin, Jingmin, Kiner, Evgeny, Lieberman, Judy, McReynolds, Larry   +5 more
core   +1 more source