Results 1 to 10 of about 76,196 (249)

Two-metal versus one-metal mechanisms of lysine adenylylation by ATP-dependent and NAD⁺-dependent polynucleotide ligases. [PDF]

Proc Natl Acad Sci U S A, 2017
Significance This season marks the 50th anniversary of the discovery of polynucleotide ligases, the sine qua non enzymes of nucleic acid repair and the enabling reagents of molecular biology, reported in a series of seminal papers in PNAS.
Unciuleac MC, Goldgur Y, Shuman S.
europepmc   +4 more sources

Polynucleotide 3′-terminal Phosphate Modifications by RNA and DNA Ligases [PDF]

Journal of Biological Chemistry, 2014
RNA and DNA ligases catalyze the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl ends of nucleic acids. In this work, we describe the ability of the thermophilic RNA ligase MthRnl from Methanobacterium thermoautotrophicum to recognize and modify the 3'-terminal phosphate of RNA and single-stranded DNA (ssDNA).
Alexander Zhelkovsky, Larry McReynolds
semanticscholar   +5 more sources

Two distinct polynucleotide ligases from rat liver [PDF]

FEBS Letters, 1975
Kinji Tsukada, Hirobumi Teraoka, Mari Shimoyachi   +2 more
core   +6 more sources

RNA ligase RtcB splices 3′-phosphate and 5′-OH ends via covalent RtcB-(histidinyl)-GMP and polynucleotide-(3′)pp(5′)G intermediates [PDF]

Proceedings of the National Academy of Sciences of the United States of America, 2012
A cherished tenet of nucleic acid enzymology holds that synthesis of polynucleotide 3′-5′ phosphodiesters proceeds via the attack of a 3′-OH on a high-energy 5′ phosphoanhydride: either a nucleoside 5′-triphosphate in the case of RNA/DNA polymerases or ...
Anupam K. Chakravarty, Roman I. Subbotin, Brian T. Chait, Stewart Shuman   +3 more
openalex   +2 more sources

POLYNUCLEOTIDE LIGASE IN BACTERIOPHAGE T4D RECOMBINATION [PDF]

Genetics, 1972
ABSTRACT Following infection of E. coli B with ligase-deficient rII bacteriophage T4D recombination between linked markers is increased 4.2 fold and heterozygote frequency increased 2.3 fold. In such infection recombination occurs at a rapid rate for an extended period.
Henry Krisch, Nancy V. Hamlett, Hillard Berger   +2 more
openalex   +4 more sources

Direct comparison of nick-joining activity of the nucleic acid ligases from bacteriophage T4 [PDF]

Biochemical Journal, 2006
The genome of bacteriophage T4 encodes three polynucleotide ligases, which seal the backbone of nucleic acids during infection of host bacteria. The T4Dnl (T4 DNA ligase) and two RNA ligases [T4Rnl1 (T4 RNA ligase 1) and T4Rnl2] join a diverse array of ...
Amitsur, Arabshahi, Bebenek, Begley, Bellacosa, Blondal, Blondal, Cherepanov, Cherepanov, Clepet, Della, Desmond R. Bullard, Doherty, Engler, Fareed, Higgins, Ho, Ho, Kaufmann, Keppetipola, Kornberg, Kuhn, Lavesa-Curto, Lehman, Markham, Martins, Martins, Miller, Moore, Nandakumar, Nandakumar, Nandakumar, Nick McElhinny, Omari, Pascal, Pitcher, Pitcher, Richard P. Bowater, Rossi, Ruiz, Runnels, Rydberg, Sambrook, Sekiguchi, Shuman, Shuman, Snopek, Sriskanda, Stuart, Timson, Tomkinson, Wang, Wang, Weiss, Wilkinson, Wilkinson, Wilkinson, Wood, Wood, Worthey, Yin, Yin   +61 more
core   +5 more sources

Studies on Polynucleotides, C A Novel Joining Reaction Catalyzed by the T4-Polynucleotide Ligase [PDF]

Proceedings of the National Academy of Sciences, 1970
The polynucleotide ligase isolated from T4-infected Escherichia coli was previously shown to bring about repair of breaks in the single strands of bihelical DNA. The present work shows that the enzyme can also catalyze the joining of DNA duplexes at their base-paired ends.
V. Sgaramella, J.H. van de Sande, H. G. Khorana   +2 more
openalex   +4 more sources

Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay [PDF]

Frontiers in Genetics, 2014
Base excision repair (BER) is the predominant cellular mechanism by which human cells repair DNA base damage, sites of base loss and DNA single strand breaks of various complexity, that are generated in their thousands in every human cell per day as a ...
Jason Luke Parsons, Catherine Marie Nickson   +1 more
doaj   +3 more sources

Characterization of a Baculovirus Enzyme with RNA Ligase, Polynucleotide 5′-Kinase, and Polynucleotide 3′-Phosphatase Activities [PDF]

Journal of Biological Chemistry, 2004
The end-healing and end-sealing steps of the phage T4-induced RNA restriction-repair pathway are performed by two separate enzymes, a bifunctional polynucleotide 5'-kinase/3'-phosphatase and an ATP-dependent RNA ligase. Here we show that a single trifunctional baculovirus enzyme, RNA ligase 1 (Rnl1), catalyzes the identical set of RNA repair reactions.
Alexandra Martins, Stewart Shuman
openalex   +5 more sources

A Mutant of Bacteriophage T7 Deficient in Polynucleotide Ligase

Journal of Biological Chemistry, 1971
Abstract An amber mutant of bacteriophage T7, obtained by mutagenesis with hydroxylamine, fails to induce the T7 polynucleotide ligase upon infection of Escherichia coli. The mutant (T7amHA13) grows normally on a suppressor-negative host; its sensitivity to ultraviolet light is the same as that of wild type T7.
Yukito Masamune, G Frenkel, Charles C. Richardson   +2 more
openalex   +3 more sources