Results 1 to 10 of about 19 (18)
An acidic patch in the unstructured N‐terminus modulates LSD1 activity
Protein Science, Volume 34, Issue 12, December 2025.Abstract Lysine‐specific demethylase 1 (LSD1) plays a crucial role in chromatin organization and gene regulation by removing methyl groups from histone and non‐histone substrates. While its catalytic core is well characterized, the functional contributions of its intrinsically disordered N‐terminal region remain less understood.
Franziska Dukatz +3 more
wiley +1 more source
Optimizing (Bio) Catalysis with Liquid‐Liquid Phase Separation Systems
ChemSystemsChem, Volume 7, Issue 6, November 2025.This review highlights how LLPS creates membraneless compartments involved in different scenarios in nature. It further explores different examples that focus on the development of synthetic coacervates that enhance catalytic efficiency by concentrating reactants, or by the direct influence of the coacervates building blocks themselves.
David Q. P. Reis +4 more
wiley +1 more source
A CRISPRi Gene Regulation System for Bifidobacteria
Microbial Biotechnology, Volume 18, Issue 11, November 2025.We report the development of a single plasmid‐based CRISPR interference system based on a catalytically dead Cas9 from Streptococcus thermophilus for targeted gene repression in bifidobacteria. This system is capable of repressing genes in a diverse range of bifidobacteria enabling functional genomics studies in non‐model strains.
Lisa Friess +2 more
wiley +1 more source
Molecular Ecology Resources, Volume 25, Issue 8, November 2025.ABSTRACT Ancient DNA (aDNA) analysis of archaeological dental calculus has provided a wealth of insights into ancient health, demography and lifestyles. However, the workflow for ancient metagenomics is still evolving, raising concerns about reproducibility.
Sterling L. Wright +8 more
wiley +1 more source
Journal of Integrative Plant Biology, Volume 67, Issue 11, Page 2982-2998, November 2025.The Arabidopsis bZIP3 protein works in conjunction with ASYMMETRIC LEAVES1 to facilitate the function of the CONSTANS protein in activating FLOWERING LOCUS T expression at the end of the day in a day length‐dependent manner. ABSTRACT Plants monitor daylength to synchronize their flowering time with their surroundings and thus maximize reproductive ...
Wonbok Lee +10 more
wiley +1 more source
A Cas12a Toolbox for Rapid and Flexible Group B Streptococcus Genomic Editing and CRISPRi
Molecular Microbiology, Volume 124, Issue 5, Page 449-461, November 2025.We developed a resource set and protocols that allow researchers to quickly alter or silence genes in Group B Streptococcus. Using flexible plasmids and a drug‐inducible Cas12a system, the approach enables efficient generation of insertions, deletions, or knockdowns. These tools are publicly available.
G. H. Hillebrand +5 more
wiley +1 more source
Molecular Oncology, Volume 19, Issue 10, Page 2833-2859, October 2025.We identified adaptor protein ShcD as upregulated in triple‐negative breast cancer and found its expression to be correlated with reduced patient survival and increased invasion in cell models. Using a proteomic screen, we identified novel ShcD binding partners involved in EGFR signaling pathways.
Hayley R. Lau +11 more
wiley +1 more source
Remote Control of Eukaryotic Gene Expression by a Modular Ultrasound‐Responsive RNA Toolkit
Angewandte Chemie, Volume 137, Issue 29, July 14, 2025.A modular RNA‐based sonogenetic toolkit enables remote control of gene expression in eukaryotes by releasing small molecule modulators from ultrasound‐responsive carriers. These modulators activate engineered mRNA elements such as riboswitches, leading to tunable protein expression.
Fahimeh Charbgoo +7 more
wiley +2 more sources
ChemCatChem, Volume 17, Issue 20, October 20, 2025.Thioxanthone is an efficient visible‐light photosensitizer and facilitates reactions that are not known from natural enzymes. It is now possible to incorporate thioxanthone as a noncanonical amino acid (thioX) into proteins by an engineered amino acyl tRNA synthetase.
Marco Seifert +3 more
wiley +1 more source
The Lrs14‐Like AbfR1 Homolog From Metallosphaera sedula Is a Nucleoid‐Organizing Protein
MicrobiologyOpen, Volume 14, Issue 5, October 2025.Nucleoid organization in Crenarchaeota involves diverse small DNA‐binding proteins. The Lrs14‐type protein AbfR1 from Metallosphaera sedula binds nonsequence specifically across the genome and induces DNA condensation. These findings suggest a structural role for AbfR1Ms in chromatin architecture, functionally resembling bacterial nucleoid‐associated ...
Veerke De Kock +5 more
wiley +1 more source