Results 1 to 10 of about 3,754 (164)

Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay [PDF]

Frontiers in Genetics, 2014
Base excision repair (BER) is the predominant cellular mechanism by which human cells repair DNA base damage, sites of base loss and DNA single strand breaks of various complexity, that are generated in their thousands in every human cell per day as a ...
Jason Luke Parsons, Catherine Marie Nickson   +1 more
doaj   +5 more sources

Polynucleotide 3′-terminal Phosphate Modifications by RNA and DNA Ligases [PDF]

Journal of Biological Chemistry, 2014
RNA and DNA ligases catalyze the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl ends of nucleic acids. In this work, we describe the ability of the thermophilic RNA ligase MthRnl from Methanobacterium thermoautotrophicum to recognize and modify the 3'-terminal phosphate of RNA and single-stranded DNA (ssDNA).
Alexander M, Zhelkovsky, Larry A, McReynolds   +1 more
exaly   +3 more sources

Two distinct polynucleotide ligases from rat liver [PDF]

FEBS Letters, 1975
Teraoka, Hirobumi, Shimoyachi, Mari, Tsukada, Kinji   +2 more
exaly   +3 more sources

POLYNUCLEOTIDE LIGASE IN BACTERIOPHAGE T4D RECOMBINATION [PDF]

Genetics, 1972
ABSTRACT Following infection of E. coli B with ligase-deficient rII bacteriophage T4D recombination between linked markers is increased 4.2 fold and heterozygote frequency increased 2.3 fold. In such infection recombination occurs at a rapid rate for an extended period.
H M, Krisch, N V, Hamlett, H, Berger
openaire   +2 more sources

Studies on Polynucleotides, C A Novel Joining Reaction Catalyzed by the T4-Polynucleotide Ligase [PDF]

Proceedings of the National Academy of Sciences, 1970
The polynucleotide ligase isolated from T4-infected Escherichia coli was previously shown to bring about repair of breaks in the single strands of bihelical DNA. The present work shows that the enzyme can also catalyze the joining of DNA duplexes at their base-paired ends.
V, Sgaramella, J H, Van de Sande, H G, Khorana   +2 more
openaire   +2 more sources

Kinetics and Effect of Salts and Polyamines on T4 Polynucleotide Ligase [PDF]

European Journal of Biochemistry, 1975
The kinetics of T4 polynucleotide ligase has been investigated at pH 8, 20 °C and using the double‐stranded DNA substrate (dA)n [(dT)10]n/10. Double‐reciprocal plots of initial rates vs substrate concentrations as well as product inhibition studies have indicated that the enzyme reacts according to a ping‐pong mechanism. The overall mechanism was found
A J, Raae, R K, Kleppe, K, Kleppe
openaire   +2 more sources

Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]

, 2013
Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward, Brissett, Nigel C, Doherty, Aidan J   +2 more
core   +1 more source

Label-free electrochemical monitoring of DNA ligase activity [PDF]

, 2008
This study presents a simple, label-free electrochemical technique for the monitoring of DNA ligase activity. DNA ligases are enzymes that catalyze joining of breaks in the backbone of DNA and are of significant scientific interest due to their essential
Ahel I., Bellamy S. R., Borodina T. A., Bullard D. R., Cahova-Kucharikova K., Cao W. G., Doherty A. J., Fadrna R., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Fojta M., Friedberg E. C., Gowers D. M., Heiter D. F., Kucharikova K., Liu L. F., Palecek E., Pascal J. M., Rass U., Schrock R. D., Scott B. O. S., Shuman S., Tomkinson A. E., Vacek J., Wilkinson A., Wilkinson A., Zauner G.   +29 more
core   +1 more source

Characterization of a Baculovirus Enzyme with RNA Ligase, Polynucleotide 5′-Kinase, and Polynucleotide 3′-Phosphatase Activities [PDF]

Journal of Biological Chemistry, 2004
The end-healing and end-sealing steps of the phage T4-induced RNA restriction-repair pathway are performed by two separate enzymes, a bifunctional polynucleotide 5'-kinase/3'-phosphatase and an ATP-dependent RNA ligase. Here we show that a single trifunctional baculovirus enzyme, RNA ligase 1 (Rnl1), catalyzes the identical set of RNA repair reactions.
Alexandra, Martins, Stewart, Shuman
openaire   +2 more sources

T4 RNA Ligase 2 truncated active site mutants: improved tools for RNA analysis [PDF]

, 2011
Background T4 RNA ligases 1 and 2 are useful tools for RNA analysis. Their use upstream of RNA analyses such as high-throughput RNA sequencing and microarrays has recently increased their importance.
Sebastien Viollet, Ryan T Fuchs, Daniela B Munafo, Fanglei Zhuang, Gregory B Robb   +4 more
core   +1 more source