Results 1 to 10 of about 2,008 (137)

Polynucleotide 3′-terminal Phosphate Modifications by RNA and DNA Ligases [PDF]

Journal of Biological Chemistry, 2014
RNA and DNA ligases catalyze the formation of a phosphodiester bond between the 5'-phosphate and 3'-hydroxyl ends of nucleic acids. In this work, we describe the ability of the thermophilic RNA ligase MthRnl from Methanobacterium thermoautotrophicum to recognize and modify the 3'-terminal phosphate of RNA and single-stranded DNA (ssDNA).
Alexander M, Zhelkovsky, Larry A, McReynolds   +1 more
openaire   +4 more sources

Global Quantitative Analysis of Ligation Reactions in Self-Assembled DNA Nanostructures at the Single-Nick Level. [PDF]

Small
Ligations of staple strands by ligase at 64 nick sites on DNA origami can be quantitatively mapped. While ligations preferentially occur at the trapezoid edges rather than at inner sites, excellent agreement between the experimental ligation yields and docking simulations suggests that this is a result of variations in the ligase docking probability ...
Hacker K, Juricke E, Münch C, Suma A, Keller A, Zhang Y.   +5 more
europepmc   +2 more sources

Two distinct polynucleotide ligases from rat liver [PDF]

FEBS Letters, 1975
Teraoka, Hirobumi, Shimoyachi, Mari, Tsukada, Kinji   +2 more
openaire   +4 more sources

POLYNUCLEOTIDE LIGASE IN BACTERIOPHAGE T4D RECOMBINATION [PDF]

Genetics, 1972
ABSTRACT Following infection of E. coli B with ligase-deficient rII bacteriophage T4D recombination between linked markers is increased 4.2 fold and heterozygote frequency increased 2.3 fold. In such infection recombination occurs at a rapid rate for an extended period.
H M, Krisch, N V, Hamlett, H, Berger
openaire   +2 more sources

Studies on Polynucleotides, C A Novel Joining Reaction Catalyzed by the T4-Polynucleotide Ligase [PDF]

Proceedings of the National Academy of Sciences, 1970
The polynucleotide ligase isolated from T4-infected Escherichia coli was previously shown to bring about repair of breaks in the single strands of bihelical DNA. The present work shows that the enzyme can also catalyze the joining of DNA duplexes at their base-paired ends. This novel reaction
V, Sgaramella, J H, Van de Sande, H G, Khorana   +2 more
openaire   +2 more sources

Monitoring regulation of DNA repair activities of cultured cells in-gel using the comet assay

Frontiers in Genetics, 2014
Base excision repair (BER) is the predominant cellular mechanism by which human cells repair DNA base damage, sites of base loss and DNA single strand breaks of various complexity, that are generated in their thousands in every human cell per day as a ...
Jason Luke Parsons, Catherine Marie Nickson   +1 more
doaj   +1 more source

Kinetics and Effect of Salts and Polyamines on T4 Polynucleotide Ligase [PDF]

European Journal of Biochemistry, 1975
The kinetics of T4 polynucleotide ligase has been investigated at pH 8, 20 °C and using the double‐stranded DNA substrate (dA)n [(dT)10]n/10. Double‐reciprocal plots of initial rates vs substrate concentrations as well as product inhibition studies have indicated that the enzyme reacts according to a ping‐pong mechanism. The overall mechanism was found
A J, Raae, R K, Kleppe, K, Kleppe
openaire   +2 more sources

Characterization of a Baculovirus Enzyme with RNA Ligase, Polynucleotide 5′-Kinase, and Polynucleotide 3′-Phosphatase Activities [PDF]

Journal of Biological Chemistry, 2004
The end-healing and end-sealing steps of the phage T4-induced RNA restriction-repair pathway are performed by two separate enzymes, a bifunctional polynucleotide 5'-kinase/3'-phosphatase and an ATP-dependent RNA ligase. Here we show that a single trifunctional baculovirus enzyme, RNA ligase 1 (Rnl1), catalyzes the identical set of RNA repair reactions.
Alexandra, Martins, Stewart, Shuman
openaire   +2 more sources

A Mutant of Bacteriophage T7 Deficient in Polynucleotide Ligase

Journal of Biological Chemistry, 1971
Abstract An amber mutant of bacteriophage T7, obtained by mutagenesis with hydroxylamine, fails to induce the T7 polynucleotide ligase upon infection of Escherichia coli. The mutant (T7amHA13) grows normally on a suppressor-negative host; its sensitivity to ultraviolet light is the same as that of wild type T7.
Y, Masamune, G D, Frenkel, C C, Richardson   +2 more
openaire   +2 more sources

Polynucleotide Ligase Activity of Eukaryotic Topoisomerase I [PDF]

Molecular Cell, 1998
Introduction of a single ribonucleoside immediately 5' of the scissile phosphate of a duplex DNA substrate converts eukaryotic topoisomerase I into an endoribonuclease. Here, I demonstrate that the RNase reaction is reversible. Vaccinia topoisomerase can ligate 2', 3'-cyclic phosphate and 5'-hydroxyl termini annealed to a bridging template strand ...
openaire   +2 more sources